Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2016 and GFF2017 are separated by 36 nucleotides GFF2017 and GFF2032 are separated by 115 nucleotides GFF2032 and GFF2018 are separated by 247 nucleotides GFF2018 and GFF2019 are separated by 71 nucleotides
GFF2016 - Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8), at 27,389 to 29,092
GFF2016
GFF2017 - Transcriptional regulator, TetR family, at 29,129 to 29,704
GFF2017
GFF2032 - tRNA-Phe-GAA, at 29,820 to 29,895
GFF2032
GFF2018 - transcriptional regulator, MerR family, at 30,143 to 30,475
GFF2018
GFF2019 - FIG01049542: hypothetical protein, at 30,547 to 30,924
GFF2019
Position (kb)
29
30 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 29.144 kb on - strand at 29.194 kb on + strand, within GFF2017 at 29.261 kb on - strand, within GFF2017 at 29.275 kb on - strand, within GFF2017 at 29.275 kb on - strand, within GFF2017 at 29.291 kb on + strand, within GFF2017 at 29.308 kb on - strand, within GFF2017 at 29.312 kb on - strand, within GFF2017 at 29.402 kb on - strand, within GFF2017 at 29.468 kb on - strand, within GFF2017 at 29.512 kb on + strand, within GFF2017 at 29.635 kb on + strand, within GFF2017 at 29.758 kb on - strand at 29.916 kb on + strand at 29.976 kb on + strand at 29.977 kb on - strand at 29.977 kb on - strand at 29.980 kb on + strand at 29.982 kb on + strand at 29.982 kb on + strand at 30.061 kb on - strand at 30.206 kb on + strand, within GFF2018 at 30.206 kb on + strand, within GFF2018 at 30.207 kb on - strand, within GFF2018 at 30.207 kb on - strand, within GFF2018 at 30.269 kb on + strand, within GFF2018 at 30.270 kb on - strand, within GFF2018 at 30.270 kb on - strand, within GFF2018 at 30.344 kb on - strand, within GFF2018 at 30.439 kb on + strand, within GFF2018 at 30.540 kb on + strand at 30.541 kb on - strand at 30.551 kb on + strand at 30.558 kb on - strand at 30.663 kb on + strand, within GFF2019 at 30.664 kb on - strand, within GFF2019 at 30.664 kb on - strand, within GFF2019 at 30.674 kb on - strand, within GFF2019 at 30.887 kb on + strand at 30.892 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 29,144 - +0.4 29,194 + GFF2017 0.11 +0.0 29,261 - GFF2017 0.23 -0.9 29,275 - GFF2017 0.25 +2.5 29,275 - GFF2017 0.25 +1.6 29,291 + GFF2017 0.28 -0.2 29,308 - GFF2017 0.31 -1.4 29,312 - GFF2017 0.32 -0.2 29,402 - GFF2017 0.47 +0.8 29,468 - GFF2017 0.59 -0.1 29,512 + GFF2017 0.66 +1.0 29,635 + GFF2017 0.88 -0.4 29,758 - -1.5 29,916 + +0.1 29,976 + -0.5 29,977 - +0.9 29,977 - +0.9 29,980 + -0.5 29,982 + -0.8 29,982 + -0.6 30,061 - -0.5 30,206 + GFF2018 0.19 +0.0 30,206 + GFF2018 0.19 -1.7 30,207 - GFF2018 0.19 +1.0 30,207 - GFF2018 0.19 +0.1 30,269 + GFF2018 0.38 -0.5 30,270 - GFF2018 0.38 -0.5 30,270 - GFF2018 0.38 +1.3 30,344 - GFF2018 0.60 -0.4 30,439 + GFF2018 0.89 +0.4 30,540 + -0.4 30,541 - -1.1 30,551 + +0.2 30,558 - -2.1 30,663 + GFF2019 0.31 -0.4 30,664 - GFF2019 0.31 +1.4 30,664 - GFF2019 0.31 -1.4 30,674 - GFF2019 0.34 -1.2 30,887 + +0.3 30,892 + -2.6
Or see this region's nucleotide sequence