Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2027

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2025 and GFF2026 overlap by 4 nucleotidesGFF2026 and GFF2027 are separated by 394 nucleotidesGFF2027 and GFF2028 are separated by 264 nucleotides GFF2025 - FIG001353: Acetyltransferase, at 33,133 to 33,624 GFF2025 GFF2026 - FIG032766: hypothetical protein, at 33,621 to 33,914 GFF2026 GFF2027 - putative cytoplasmic protein, at 34,309 to 34,452 GFF2027 GFF2028 - Type III secretion and flagellar regulator RtsA, at 34,717 to 35,592 GFF2028 Position (kb) 34 35Strain fitness (log2 ratio) -2 -1 0 1at 33.396 kb on + strand, within GFF2025at 33.456 kb on - strand, within GFF2025at 33.467 kb on - strand, within GFF2025at 33.550 kb on - strand, within GFF2025at 33.553 kb on + strand, within GFF2025at 33.554 kb on - strand, within GFF2025at 33.582 kb on + strandat 33.583 kb on - strandat 33.583 kb on - strandat 33.914 kb on + strandat 33.931 kb on - strandat 33.931 kb on - strandat 33.946 kb on - strandat 33.951 kb on + strandat 33.951 kb on + strandat 34.115 kb on + strandat 34.141 kb on + strandat 34.165 kb on - strandat 34.293 kb on - strandat 35.132 kb on + strand, within GFF2028at 35.133 kb on - strand, within GFF2028

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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33,396 + GFF2025 0.53 -2.3
33,456 - GFF2025 0.66 +0.0
33,467 - GFF2025 0.68 +1.0
33,550 - GFF2025 0.85 -1.1
33,553 + GFF2025 0.85 +0.7
33,554 - GFF2025 0.86 +0.1
33,582 + +0.0
33,583 - +0.6
33,583 - +1.5
33,914 + -1.1
33,931 - +1.6
33,931 - +0.2
33,946 - -0.4
33,951 + -0.5
33,951 + +0.2
34,115 + +0.4
34,141 + +1.6
34,165 - -0.9
34,293 - -0.4
35,132 + GFF2028 0.47 +0.4
35,133 - GFF2028 0.47 -1.1

Or see this region's nucleotide sequence