Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1944

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1942 and GFF1943 are separated by 66 nucleotidesGFF1943 and GFF1944 are separated by 142 nucleotidesGFF1944 and GFF1945 are separated by 159 nucleotides GFF1942 - FIG00545237: hypothetical protein, at 133,673 to 134,191 GFF1942 GFF1943 - Putative inner membrane protein, at 134,258 to 134,869 GFF1943 GFF1944 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASI (EC 2.3.1.41), at 135,012 to 136,226 GFF1944 GFF1945 - tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (EC 2.1.1.61) / FAD-dependent cmnm(5)s(2)U34 oxidoreductase, at 136,386 to 138,386 GFF1945 Position (kb) 135 136 137Strain fitness (log2 ratio) -2 -1 0 1at 134.120 kb on + strand, within GFF1942at 134.645 kb on - strand, within GFF1943at 134.990 kb on + strandat 134.993 kb on + strandat 135.004 kb on - strandat 136.378 kb on + strandat 136.384 kb on - strandat 136.402 kb on + strandat 136.561 kb on + strandat 136.562 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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134,120 + GFF1942 0.86 -0.0
134,645 - GFF1943 0.63 -1.3
134,990 + +0.8
134,993 + -1.8
135,004 - +0.3
136,378 + -0.2
136,384 - +0.0
136,402 + -0.2
136,561 + -0.0
136,562 - -0.1

Or see this region's nucleotide sequence