Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1935 and GFF1936 are separated by 67 nucleotides GFF1936 and GFF1937 are separated by 103 nucleotides
GFF1935 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 127,664 to 128,677
GFF1935
GFF1936 - Erythronate-4-phosphate dehydrogenase (EC 1.1.1.290), at 128,745 to 129,881
GFF1936
GFF1937 - Cell division protein, at 129,985 to 130,986
GFF1937
Position (kb) 128
129
130 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 127.799 kb on - strand, within GFF1935 at 127.868 kb on - strand, within GFF1935 at 127.953 kb on + strand, within GFF1935 at 128.032 kb on + strand, within GFF1935 at 128.084 kb on - strand, within GFF1935 at 128.206 kb on + strand, within GFF1935 at 128.306 kb on - strand, within GFF1935 at 128.397 kb on - strand, within GFF1935 at 128.697 kb on - strand at 128.700 kb on + strand at 128.700 kb on + strand at 128.900 kb on + strand, within GFF1936 at 128.933 kb on - strand, within GFF1936 at 128.987 kb on + strand, within GFF1936 at 128.987 kb on + strand, within GFF1936 at 128.988 kb on - strand, within GFF1936 at 129.015 kb on + strand, within GFF1936 at 129.061 kb on + strand, within GFF1936 at 129.108 kb on - strand, within GFF1936 at 129.162 kb on - strand, within GFF1936 at 129.334 kb on + strand, within GFF1936 at 129.350 kb on - strand, within GFF1936 at 129.525 kb on + strand, within GFF1936 at 129.528 kb on - strand, within GFF1936 at 129.589 kb on + strand, within GFF1936 at 129.605 kb on + strand, within GFF1936 at 129.611 kb on + strand, within GFF1936 at 129.611 kb on + strand, within GFF1936 at 129.611 kb on + strand, within GFF1936 at 129.612 kb on - strand, within GFF1936 at 129.816 kb on + strand at 129.838 kb on - strand at 129.949 kb on - strand at 129.951 kb on - strand at 129.980 kb on + strand at 129.984 kb on + strand at 129.984 kb on + strand at 130.143 kb on - strand, within GFF1937 at 130.287 kb on - strand, within GFF1937 at 130.486 kb on - strand, within GFF1937 at 130.612 kb on - strand, within GFF1937 at 130.655 kb on + strand, within GFF1937 at 130.673 kb on + strand, within GFF1937 at 130.677 kb on + strand, within GFF1937 at 130.677 kb on + strand, within GFF1937 at 130.678 kb on - strand, within GFF1937 at 130.738 kb on + strand, within GFF1937 at 130.738 kb on + strand, within GFF1937 at 130.739 kb on - strand, within GFF1937 at 130.806 kb on + strand, within GFF1937 at 130.829 kb on + strand, within GFF1937
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 127,799 - GFF1935 0.13 -0.0 127,868 - GFF1935 0.20 -0.8 127,953 + GFF1935 0.29 +4.7 128,032 + GFF1935 0.36 +3.6 128,084 - GFF1935 0.41 +0.8 128,206 + GFF1935 0.53 +4.8 128,306 - GFF1935 0.63 +1.5 128,397 - GFF1935 0.72 +0.9 128,697 - +0.5 128,700 + +3.2 128,700 + +3.9 128,900 + GFF1936 0.14 +1.0 128,933 - GFF1936 0.17 -2.6 128,987 + GFF1936 0.21 -0.4 128,987 + GFF1936 0.21 -1.4 128,988 - GFF1936 0.21 -0.8 129,015 + GFF1936 0.24 -2.5 129,061 + GFF1936 0.28 -1.7 129,108 - GFF1936 0.32 -1.2 129,162 - GFF1936 0.37 -2.1 129,334 + GFF1936 0.52 -2.5 129,350 - GFF1936 0.53 -2.7 129,525 + GFF1936 0.69 +1.9 129,528 - GFF1936 0.69 -1.6 129,589 + GFF1936 0.74 -2.3 129,605 + GFF1936 0.76 -1.0 129,611 + GFF1936 0.76 -0.2 129,611 + GFF1936 0.76 -1.0 129,611 + GFF1936 0.76 +0.2 129,612 - GFF1936 0.76 -1.7 129,816 + -2.4 129,838 - -3.9 129,949 - -0.6 129,951 - -0.1 129,980 + -1.2 129,984 + -1.3 129,984 + +1.0 130,143 - GFF1937 0.16 -1.2 130,287 - GFF1937 0.30 +1.5 130,486 - GFF1937 0.50 +1.3 130,612 - GFF1937 0.63 -0.2 130,655 + GFF1937 0.67 -0.2 130,673 + GFF1937 0.69 +0.2 130,677 + GFF1937 0.69 -1.7 130,677 + GFF1937 0.69 -1.8 130,678 - GFF1937 0.69 +0.2 130,738 + GFF1937 0.75 -0.6 130,738 + GFF1937 0.75 -0.5 130,739 - GFF1937 0.75 +1.0 130,806 + GFF1937 0.82 +2.8 130,829 + GFF1937 0.84 -0.6
Or see this region's nucleotide sequence