Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1932

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1931 and GFF1932 are separated by 67 nucleotidesGFF1932 and GFF1933 are separated by 149 nucleotidesGFF1933 and GFF1934 are separated by 48 nucleotides GFF1931 - Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17), at 123,744 to 125,012 GFF1931 GFF1932 - Acetyl-coenzyme A carboxyl transferase beta chain (EC 6.4.1.2), at 125,080 to 125,994 GFF1932 GFF1933 - DedA protein, at 126,144 to 126,803 GFF1933 GFF1934 - tRNA pseudouridine synthase A (EC 4.2.1.70), at 126,852 to 127,664 GFF1934 Position (kb) 125 126Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 125.018 kb on - strandat 126.032 kb on - strandat 126.138 kb on + strandat 126.240 kb on - strand, within GFF1933at 126.327 kb on - strand, within GFF1933at 126.327 kb on - strand, within GFF1933at 126.327 kb on - strand, within GFF1933at 126.328 kb on + strand, within GFF1933at 126.786 kb on - strandat 126.853 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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125,018 - -0.8
126,032 - +3.4
126,138 + +3.3
126,240 - GFF1933 0.15 -3.8
126,327 - GFF1933 0.28 -2.3
126,327 - GFF1933 0.28 -2.7
126,327 - GFF1933 0.28 -1.5
126,328 + GFF1933 0.28 -4.9
126,786 - -3.6
126,853 + -2.8

Or see this region's nucleotide sequence