Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF190 and GFF191 are separated by 2 nucleotides GFF191 and GFF192 are separated by 10 nucleotides GFF192 and GFF193 are separated by 105 nucleotides GFF193 and GFF194 are separated by 198 nucleotides
GFF190 - SSU rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase (EC 2.1.1.182), at 185,606 to 186,427
GFF190
GFF191 - ApaG protein, at 186,430 to 186,807
GFF191
GFF192 - 'Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41)' transl_table=11, at 186,818 to 187,666
GFF192
GFF193 - Dihydrofolate reductase (EC 1.5.1.3), at 187,772 to 188,251
GFF193
GFF194 - Glutathione-regulated potassium-efflux system protein KefC, at 188,450 to 190,312
GFF194
Position (kb) 186
187
188 Strain fitness (log2 ratio) -1
0
1
2
3 at 185.867 kb on + strand, within GFF190 at 185.957 kb on - strand, within GFF190 at 185.990 kb on + strand, within GFF190 at 185.991 kb on - strand, within GFF190 at 185.992 kb on + strand, within GFF190 at 186.020 kb on - strand, within GFF190 at 186.074 kb on + strand, within GFF190 at 186.074 kb on + strand, within GFF190 at 186.105 kb on - strand, within GFF190 at 186.392 kb on + strand at 186.585 kb on + strand, within GFF191 at 186.811 kb on + strand at 186.855 kb on - strand at 186.892 kb on + strand at 186.892 kb on + strand at 186.963 kb on + strand, within GFF192 at 186.964 kb on - strand, within GFF192 at 186.969 kb on - strand, within GFF192 at 186.989 kb on - strand, within GFF192 at 187.064 kb on - strand, within GFF192 at 187.183 kb on + strand, within GFF192 at 187.336 kb on - strand, within GFF192 at 187.346 kb on - strand, within GFF192 at 187.346 kb on - strand, within GFF192 at 187.348 kb on + strand, within GFF192 at 187.400 kb on + strand, within GFF192 at 187.478 kb on + strand, within GFF192 at 187.479 kb on - strand, within GFF192 at 187.479 kb on - strand, within GFF192 at 187.605 kb on - strand at 187.605 kb on - strand at 188.288 kb on - strand at 188.391 kb on - strand at 188.391 kb on - strand at 188.414 kb on + strand at 188.415 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 185,867 + GFF190 0.32 +0.7 185,957 - GFF190 0.43 +0.8 185,990 + GFF190 0.47 -0.9 185,991 - GFF190 0.47 +2.8 185,992 + GFF190 0.47 +1.1 186,020 - GFF190 0.50 +0.5 186,074 + GFF190 0.57 +0.8 186,074 + GFF190 0.57 -0.7 186,105 - GFF190 0.61 +1.7 186,392 + +0.1 186,585 + GFF191 0.41 +1.0 186,811 + +1.6 186,855 - +3.6 186,892 + +2.3 186,892 + +1.3 186,963 + GFF192 0.17 +2.7 186,964 - GFF192 0.17 +2.9 186,969 - GFF192 0.18 +2.5 186,989 - GFF192 0.20 +2.4 187,064 - GFF192 0.29 +2.5 187,183 + GFF192 0.43 +2.0 187,336 - GFF192 0.61 +3.1 187,346 - GFF192 0.62 +3.3 187,346 - GFF192 0.62 +2.6 187,348 + GFF192 0.62 +1.6 187,400 + GFF192 0.69 +3.5 187,478 + GFF192 0.78 +1.4 187,479 - GFF192 0.78 +3.1 187,479 - GFF192 0.78 +0.4 187,605 - +1.7 187,605 - +1.8 188,288 - -0.1 188,391 - +0.5 188,391 - -0.4 188,414 + -0.6 188,415 - +0.0
Or see this region's nucleotide sequence