Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1902 and GFF1903 are separated by 48 nucleotides GFF1903 and GFF1904 overlap by 8 nucleotides GFF1904 and GFF1905 overlap by 4 nucleotides
GFF1902 - Putative S-transferase, at 97,970 to 99,490
GFF1902
GFF1903 - Transketolase, C-terminal section (EC 2.2.1.1), at 99,539 to 100,492
GFF1903
GFF1904 - Transketolase, N-terminal section (EC 2.2.1.1), at 100,485 to 101,315
GFF1904
GFF1905 - FIG00732228: membrane protein, at 101,312 to 102,703
GFF1905
Position (kb) 100
101
102 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 99.529 kb on - strand at 99.540 kb on + strand at 99.887 kb on - strand, within GFF1903 at 99.887 kb on - strand, within GFF1903 at 99.887 kb on - strand, within GFF1903 at 99.893 kb on - strand, within GFF1903 at 99.954 kb on + strand, within GFF1903 at 99.958 kb on + strand, within GFF1903 at 99.959 kb on - strand, within GFF1903 at 99.983 kb on - strand, within GFF1903 at 100.023 kb on - strand, within GFF1903 at 100.111 kb on + strand, within GFF1903 at 100.174 kb on + strand, within GFF1903 at 100.240 kb on + strand, within GFF1903 at 100.240 kb on + strand, within GFF1903 at 100.241 kb on - strand, within GFF1903 at 100.307 kb on - strand, within GFF1903 at 100.374 kb on - strand, within GFF1903 at 100.393 kb on + strand, within GFF1903 at 100.466 kb on - strand at 100.487 kb on - strand at 100.672 kb on - strand, within GFF1904 at 100.922 kb on + strand, within GFF1904 at 100.949 kb on + strand, within GFF1904 at 101.048 kb on + strand, within GFF1904 at 101.095 kb on + strand, within GFF1904 at 101.127 kb on + strand, within GFF1904 at 101.558 kb on + strand, within GFF1905 at 101.561 kb on + strand, within GFF1905 at 101.561 kb on + strand, within GFF1905 at 101.569 kb on + strand, within GFF1905 at 101.769 kb on + strand, within GFF1905 at 101.769 kb on + strand, within GFF1905 at 101.886 kb on - strand, within GFF1905 at 101.931 kb on + strand, within GFF1905 at 102.001 kb on + strand, within GFF1905 at 102.029 kb on - strand, within GFF1905 at 102.176 kb on - strand, within GFF1905 at 102.187 kb on + strand, within GFF1905 at 102.187 kb on + strand, within GFF1905 at 102.190 kb on - strand, within GFF1905
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 99,529 - -0.4 99,540 + +2.2 99,887 - GFF1903 0.36 +1.0 99,887 - GFF1903 0.36 +0.7 99,887 - GFF1903 0.36 +1.5 99,893 - GFF1903 0.37 -0.3 99,954 + GFF1903 0.44 -0.5 99,958 + GFF1903 0.44 +1.0 99,959 - GFF1903 0.44 -0.2 99,983 - GFF1903 0.47 +0.1 100,023 - GFF1903 0.51 -0.0 100,111 + GFF1903 0.60 +1.1 100,174 + GFF1903 0.67 -0.5 100,240 + GFF1903 0.73 -0.0 100,240 + GFF1903 0.73 +1.5 100,241 - GFF1903 0.74 +0.5 100,307 - GFF1903 0.81 -0.4 100,374 - GFF1903 0.88 -0.7 100,393 + GFF1903 0.90 +0.6 100,466 - +0.2 100,487 - +0.7 100,672 - GFF1904 0.23 +0.2 100,922 + GFF1904 0.53 -0.3 100,949 + GFF1904 0.56 -0.9 101,048 + GFF1904 0.68 -0.5 101,095 + GFF1904 0.73 +2.1 101,127 + GFF1904 0.77 +1.8 101,558 + GFF1905 0.18 -0.6 101,561 + GFF1905 0.18 -1.8 101,561 + GFF1905 0.18 -1.7 101,569 + GFF1905 0.18 -1.5 101,769 + GFF1905 0.33 +0.8 101,769 + GFF1905 0.33 -0.9 101,886 - GFF1905 0.41 +0.6 101,931 + GFF1905 0.44 -0.6 102,001 + GFF1905 0.49 +0.3 102,029 - GFF1905 0.52 -2.8 102,176 - GFF1905 0.62 +0.0 102,187 + GFF1905 0.63 +0.5 102,187 + GFF1905 0.63 -0.1 102,190 - GFF1905 0.63 +0.2
Or see this region's nucleotide sequence