Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1718 and GFF1719 are separated by 267 nucleotides GFF1719 and GFF1720 are separated by 8 nucleotides GFF1720 and GFF1721 are separated by 46 nucleotides GFF1721 and GFF1722 are separated by 59 nucleotides GFF1722 and GFF1723 are separated by 14 nucleotides
GFF1718 - hypothetical protein, at 19,025 to 19,168
GFF1718
GFF1719 - ATP synthase protein I, at 19,436 to 19,663
GFF1719
GFF1720 - ATP synthase F0 sector subunit a, at 19,672 to 20,487
GFF1720
GFF1721 - ATP synthase F0 sector subunit c (EC 3.6.3.14), at 20,534 to 20,773
GFF1721
GFF1722 - ATP synthase F0 sector subunit b (EC 3.6.3.14), at 20,833 to 21,303
GFF1722
GFF1723 - ATP synthase delta chain (EC 3.6.3.14), at 21,318 to 21,851
GFF1723
Position (kb) 19
20
21 Strain fitness (log2 ratio) -2
-1
0
1
2 at 19.014 kb on - strand at 19.063 kb on + strand, within GFF1718 at 19.140 kb on + strand, within GFF1718 at 19.218 kb on + strand at 19.318 kb on + strand at 19.318 kb on + strand at 19.350 kb on + strand at 19.432 kb on + strand at 19.435 kb on + strand at 19.435 kb on + strand at 19.435 kb on + strand at 19.474 kb on + strand, within GFF1719 at 19.474 kb on + strand, within GFF1719 at 19.474 kb on + strand, within GFF1719 at 19.548 kb on + strand, within GFF1719 at 19.572 kb on + strand, within GFF1719 at 19.644 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 19,014 - +1.4 19,063 + GFF1718 0.26 +1.0 19,140 + GFF1718 0.80 +0.4 19,218 + +2.0 19,318 + +0.1 19,318 + -0.2 19,350 + -0.1 19,432 + +1.1 19,435 + +2.0 19,435 + +1.0 19,435 + +1.0 19,474 + GFF1719 0.17 -2.2 19,474 + GFF1719 0.17 -0.5 19,474 + GFF1719 0.17 +0.8 19,548 + GFF1719 0.49 +0.4 19,572 + GFF1719 0.60 -0.3 19,644 + -0.1
Or see this region's nucleotide sequence