Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1696 and GFF1697 are separated by 66 nucleotides GFF1697 and GFF1698 overlap by 4 nucleotides GFF1698 and GFF1699 are separated by 311 nucleotides GFF1699 and GFF1700 overlap by 4 nucleotides
GFF1696 - N-3-oxohexanoyl-L-homoserine lactone quorum-sensing transcriptional activator @ N-3-oxooctanoyl-L-homoserine lactone quorum-sensing transcriptional activator, at 5,301 to 6,023
GFF1696
GFF1697 - FIG00634007: hypothetical protein, at 6,090 to 6,314
GFF1697
GFF1698 - FIG01045369: hypothetical protein, at 6,311 to 6,466
GFF1698
GFF1699 - BarA-associated response regulator UvrY (= GacA = SirA), at 6,778 to 7,434
GFF1699
GFF1700 - Excinuclease ABC subunit C, at 7,431 to 9,263
GFF1700
Position (kb) 6
7 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5.382 kb on - strand, within GFF1696 at 5.382 kb on - strand, within GFF1696 at 5.425 kb on - strand, within GFF1696 at 5.487 kb on + strand, within GFF1696 at 5.488 kb on - strand, within GFF1696 at 5.645 kb on - strand, within GFF1696 at 5.736 kb on - strand, within GFF1696 at 6.186 kb on - strand, within GFF1697 at 6.652 kb on - strand at 6.652 kb on - strand at 6.742 kb on + strand at 6.847 kb on - strand, within GFF1699 at 6.913 kb on - strand, within GFF1699 at 7.147 kb on + strand, within GFF1699 at 7.311 kb on + strand, within GFF1699 at 7.422 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 5,382 - GFF1696 0.11 +1.0 5,382 - GFF1696 0.11 +1.5 5,425 - GFF1696 0.17 +1.1 5,487 + GFF1696 0.26 +0.5 5,488 - GFF1696 0.26 +1.6 5,645 - GFF1696 0.48 -1.1 5,736 - GFF1696 0.60 +1.0 6,186 - GFF1697 0.43 -1.6 6,652 - +2.0 6,652 - +1.6 6,742 + -1.1 6,847 - GFF1699 0.11 +2.1 6,913 - GFF1699 0.21 +1.1 7,147 + GFF1699 0.56 -2.7 7,311 + GFF1699 0.81 -0.9 7,422 - -3.3
Or see this region's nucleotide sequence