Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1679 and GFF1680 are separated by 255 nucleotides GFF1680 and GFF1681 are separated by 239 nucleotides GFF1681 and GFF1682 are separated by 45 nucleotides GFF1682 and GFF1683 are separated by 169 nucleotides
GFF1679 - predicted 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (EC 5.3.1.17), at 64,343 to 64,981
GFF1679
GFF1680 - FIG00638267: hypothetical protein, at 65,237 to 65,572
GFF1680
GFF1681 - Ferritin-like protein 2, at 65,812 to 66,309
GFF1681
GFF1682 - FIG00638619: hypothetical protein, at 66,355 to 66,594
GFF1682
GFF1683 - Tyrosine-specific transport protein, at 66,764 to 68,032
GFF1683
Position (kb) 65
66
67 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 64.830 kb on - strand, within GFF1679 at 64.927 kb on - strand at 64.927 kb on - strand at 65.018 kb on - strand at 65.126 kb on + strand at 65.142 kb on - strand at 65.201 kb on + strand at 65.349 kb on - strand, within GFF1680 at 65.387 kb on + strand, within GFF1680 at 65.387 kb on + strand, within GFF1680 at 65.387 kb on + strand, within GFF1680 at 65.473 kb on + strand, within GFF1680 at 65.600 kb on + strand at 65.658 kb on - strand at 65.908 kb on + strand, within GFF1681 at 65.970 kb on + strand, within GFF1681 at 66.307 kb on + strand at 66.635 kb on + strand at 66.763 kb on - strand at 66.813 kb on + strand at 66.844 kb on + strand at 66.965 kb on + strand, within GFF1683 at 66.966 kb on - strand, within GFF1683 at 66.967 kb on + strand, within GFF1683 at 66.978 kb on + strand, within GFF1683 at 67.094 kb on + strand, within GFF1683 at 67.094 kb on + strand, within GFF1683 at 67.095 kb on - strand, within GFF1683 at 67.150 kb on + strand, within GFF1683
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 64,830 - GFF1679 0.76 +0.2 64,927 - +0.1 64,927 - -1.0 65,018 - -1.7 65,126 + +0.5 65,142 - +0.1 65,201 + +0.3 65,349 - GFF1680 0.33 +0.0 65,387 + GFF1680 0.45 -0.4 65,387 + GFF1680 0.45 +0.0 65,387 + GFF1680 0.45 -3.1 65,473 + GFF1680 0.70 -0.9 65,600 + -0.2 65,658 - -0.1 65,908 + GFF1681 0.19 -1.7 65,970 + GFF1681 0.32 -1.4 66,307 + -0.7 66,635 + +0.2 66,763 - -1.8 66,813 + -3.3 66,844 + -2.6 66,965 + GFF1683 0.16 -2.8 66,966 - GFF1683 0.16 -2.2 66,967 + GFF1683 0.16 -2.6 66,978 + GFF1683 0.17 -3.4 67,094 + GFF1683 0.26 -3.3 67,094 + GFF1683 0.26 -3.7 67,095 - GFF1683 0.26 -1.5 67,150 + GFF1683 0.30 -1.9
Or see this region's nucleotide sequence