Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1631 and GFF1632 are separated by 341 nucleotides GFF1632 and GFF1633 are separated by 123 nucleotides
GFF1631 - Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49), at 16,088 to 17,563
GFF1631
GFF1632 - Phosphogluconate repressor HexR, RpiR family, at 17,905 to 18,774
GFF1632
GFF1633 - Pyruvate kinase (EC 2.7.1.40), at 18,898 to 20,340
GFF1633
Position (kb) 17
18
19 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6 at 16.966 kb on + strand, within GFF1631 at 16.977 kb on - strand, within GFF1631 at 17.046 kb on + strand, within GFF1631 at 17.049 kb on - strand, within GFF1631 at 17.391 kb on + strand, within GFF1631 at 17.391 kb on + strand, within GFF1631 at 17.580 kb on - strand at 17.591 kb on - strand at 17.612 kb on + strand at 17.633 kb on + strand at 17.701 kb on + strand at 17.702 kb on - strand at 17.702 kb on - strand at 17.702 kb on - strand at 17.702 kb on - strand at 17.703 kb on + strand at 17.704 kb on - strand at 17.776 kb on + strand at 17.836 kb on - strand at 17.838 kb on + strand at 17.910 kb on + strand at 18.008 kb on + strand, within GFF1632 at 18.142 kb on + strand, within GFF1632 at 18.143 kb on - strand, within GFF1632 at 18.184 kb on + strand, within GFF1632 at 18.186 kb on + strand, within GFF1632 at 18.187 kb on - strand, within GFF1632 at 18.275 kb on + strand, within GFF1632 at 18.348 kb on + strand, within GFF1632 at 18.415 kb on - strand, within GFF1632 at 18.594 kb on + strand, within GFF1632 at 18.605 kb on - strand, within GFF1632 at 18.606 kb on + strand, within GFF1632 at 18.644 kb on + strand, within GFF1632 at 18.644 kb on + strand, within GFF1632 at 18.645 kb on - strand, within GFF1632 at 18.666 kb on + strand, within GFF1632 at 18.749 kb on + strand at 18.792 kb on + strand at 18.826 kb on - strand at 18.892 kb on + strand at 18.895 kb on + strand at 18.895 kb on + strand at 18.895 kb on + strand at 18.895 kb on + strand at 18.896 kb on - strand at 18.999 kb on - strand at 18.999 kb on - strand at 19.002 kb on - strand at 19.002 kb on - strand at 19.006 kb on - strand at 19.100 kb on + strand, within GFF1633 at 19.384 kb on - strand, within GFF1633 at 19.384 kb on - strand, within GFF1633 at 19.472 kb on - strand, within GFF1633
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 16,966 + GFF1631 0.59 +5.3 16,977 - GFF1631 0.60 +5.3 17,046 + GFF1631 0.65 +5.9 17,049 - GFF1631 0.65 +5.4 17,391 + GFF1631 0.88 +5.3 17,391 + GFF1631 0.88 +5.3 17,580 - +0.1 17,591 - +3.4 17,612 + +4.0 17,633 + +1.8 17,701 + -1.1 17,702 - -0.8 17,702 - -1.0 17,702 - -2.1 17,702 - -0.7 17,703 + -2.7 17,704 - -1.3 17,776 + -1.1 17,836 - -0.1 17,838 + -2.6 17,910 + -0.3 18,008 + GFF1632 0.12 -0.8 18,142 + GFF1632 0.27 -0.7 18,143 - GFF1632 0.27 -0.4 18,184 + GFF1632 0.32 -0.0 18,186 + GFF1632 0.32 -1.1 18,187 - GFF1632 0.32 -0.5 18,275 + GFF1632 0.43 -2.0 18,348 + GFF1632 0.51 -1.4 18,415 - GFF1632 0.59 +0.2 18,594 + GFF1632 0.79 -3.8 18,605 - GFF1632 0.80 +0.9 18,606 + GFF1632 0.81 -3.1 18,644 + GFF1632 0.85 -4.3 18,644 + GFF1632 0.85 -3.1 18,645 - GFF1632 0.85 +1.1 18,666 + GFF1632 0.87 -4.0 18,749 + -3.6 18,792 + -0.4 18,826 - +2.1 18,892 + +2.5 18,895 + +1.0 18,895 + +2.4 18,895 + +0.7 18,895 + +1.0 18,896 - +2.8 18,999 - +2.6 18,999 - +2.1 19,002 - +2.1 19,002 - +1.7 19,006 - +1.9 19,100 + GFF1633 0.14 +2.4 19,384 - GFF1633 0.34 +2.2 19,384 - GFF1633 0.34 +1.9 19,472 - GFF1633 0.40 -0.3
Or see this region's nucleotide sequence