Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1580 and GFF1581 are separated by 85 nucleotides GFF1581 and GFF1582 are separated by 14 nucleotides GFF1582 and GFF1583 are separated by 98 nucleotides GFF1583 and GFF1584 overlap by 4 nucleotides GFF1584 and GFF1585 overlap by 28 nucleotides
GFF1580 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 37,913 to 39,247
GFF1580
GFF1581 - 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein, at 39,333 to 39,767
GFF1581
GFF1582 - FIG01046421: hypothetical protein, at 39,782 to 39,940
GFF1582
GFF1583 - Glutaredoxin-like protein NrdH, required for reduction of Ribonucleotide reductase class Ib, at 40,039 to 40,284
GFF1583
GFF1584 - Ribonucleotide reduction protein NrdI, at 40,281 to 40,691
GFF1584
GFF1585 - Ribonucleotide reductase of class Ib (aerobic), alpha subunit (EC 1.17.4.1), at 40,664 to 42,808
GFF1585
Position (kb) 39
40 Strain fitness (log2 ratio) -2
-1
0
1
2 at 38.348 kb on + strand, within GFF1580 at 38.349 kb on - strand, within GFF1580 at 38.670 kb on - strand, within GFF1580 at 38.737 kb on + strand, within GFF1580 at 38.737 kb on + strand, within GFF1580 at 38.737 kb on + strand, within GFF1580 at 38.738 kb on - strand, within GFF1580 at 38.755 kb on + strand, within GFF1580 at 38.756 kb on - strand, within GFF1580 at 38.783 kb on + strand, within GFF1580 at 39.108 kb on + strand, within GFF1580 at 39.109 kb on - strand, within GFF1580 at 39.271 kb on + strand at 39.302 kb on + strand at 39.303 kb on - strand at 39.303 kb on - strand at 39.361 kb on - strand at 39.381 kb on + strand, within GFF1581 at 39.540 kb on - strand, within GFF1581 at 39.544 kb on + strand, within GFF1581 at 39.544 kb on + strand, within GFF1581 at 39.660 kb on + strand, within GFF1581 at 40.296 kb on + strand at 40.319 kb on + strand at 40.362 kb on - strand, within GFF1584 at 40.404 kb on + strand, within GFF1584 at 40.654 kb on + strand at 40.756 kb on + strand at 40.756 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 38,348 + GFF1580 0.33 +0.6 38,349 - GFF1580 0.33 -0.5 38,670 - GFF1580 0.57 +1.8 38,737 + GFF1580 0.62 +0.9 38,737 + GFF1580 0.62 +0.2 38,737 + GFF1580 0.62 +1.0 38,738 - GFF1580 0.62 -1.6 38,755 + GFF1580 0.63 +0.5 38,756 - GFF1580 0.63 +0.4 38,783 + GFF1580 0.65 +0.3 39,108 + GFF1580 0.90 +0.7 39,109 - GFF1580 0.90 +0.0 39,271 + -1.5 39,302 + -0.3 39,303 - -0.7 39,303 - -0.0 39,361 - +0.4 39,381 + GFF1581 0.11 +0.3 39,540 - GFF1581 0.48 -0.8 39,544 + GFF1581 0.49 -2.1 39,544 + GFF1581 0.49 +0.8 39,660 + GFF1581 0.75 -1.1 40,296 + +0.8 40,319 + +0.3 40,362 - GFF1584 0.20 -0.1 40,404 + GFF1584 0.30 +0.3 40,654 + +2.1 40,756 + +0.4 40,756 + +0.6
Or see this region's nucleotide sequence