Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1572 and GFF1573 are separated by 9 nucleotides GFF1573 and GFF1574 are separated by 78 nucleotides GFF1574 and GFF1575 are separated by 89 nucleotides GFF1575 and GFF1576 are separated by 370 nucleotides
GFF1572 - Transcriptional regulator, ArsR family, at 34,223 to 34,522
GFF1572
GFF1573 - Rhodanese-related sulfurtransferases, at 34,532 to 35,059
GFF1573
GFF1574 - corresponds to STY2919 from Accession AL513382: Salmonella typhi CT18, at 35,138 to 35,317
GFF1574
GFF1575 - DNA-binding protein H-NS, at 35,407 to 35,808
GFF1575
GFF1576 - hypothetical protein, at 36,179 to 36,292
GFF1576
Position (kb) 35
36 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 34.302 kb on + strand, within GFF1572 at 34.315 kb on + strand, within GFF1572 at 34.316 kb on - strand, within GFF1572 at 34.512 kb on - strand at 34.546 kb on - strand at 34.619 kb on + strand, within GFF1573 at 34.762 kb on - strand, within GFF1573 at 34.931 kb on - strand, within GFF1573 at 34.931 kb on - strand, within GFF1573 at 35.023 kb on - strand at 35.091 kb on + strand at 35.091 kb on + strand at 35.111 kb on + strand at 35.112 kb on - strand at 35.114 kb on - strand at 35.114 kb on - strand at 35.161 kb on - strand, within GFF1574 at 35.408 kb on + strand at 35.879 kb on + strand at 36.009 kb on - strand at 36.031 kb on + strand at 36.147 kb on - strand at 36.151 kb on + strand at 36.174 kb on + strand at 36.217 kb on + strand, within GFF1576
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 34,302 + GFF1572 0.26 -2.8 34,315 + GFF1572 0.31 +0.8 34,316 - GFF1572 0.31 -1.1 34,512 - -3.2 34,546 - -1.9 34,619 + GFF1573 0.16 +0.3 34,762 - GFF1573 0.44 -1.3 34,931 - GFF1573 0.76 -2.4 34,931 - GFF1573 0.76 -1.7 35,023 - +0.3 35,091 + -3.5 35,091 + -1.2 35,111 + -1.2 35,112 - -0.8 35,114 - -4.2 35,114 - -0.3 35,161 - GFF1574 0.13 +1.8 35,408 + +2.5 35,879 + +3.0 36,009 - -2.9 36,031 + +0.6 36,147 - -2.6 36,151 + -2.1 36,174 + -1.7 36,217 + GFF1576 0.33 +0.2
Or see this region's nucleotide sequence