Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1482 and GFF1483 are separated by 13 nucleotides GFF1483 and GFF1484 overlap by 8 nucleotides GFF1484 and GFF1485 are separated by 75 nucleotides GFF1485 and GFF1486 are separated by 83 nucleotides
GFF1482 - Anaerobic dimethyl sulfoxide reductase chain A (EC 1.8.99.-), at 152,011 to 154,440
GFF1482
GFF1483 - Anaerobic dimethyl sulfoxide reductase chain B (EC 1.8.5.3), at 154,454 to 155,080
GFF1483
GFF1484 - Anaerobic dimethyl sulfoxide reductase chain C (EC 1.8.5.3), at 155,073 to 155,846
GFF1484
GFF1485 - Putative oxidoreductase component of anaerobic dehydrogenases; Chaperone protein TorD, at 155,922 to 156,515
GFF1485
GFF1486 - Putative periplasmic or exported protein, at 156,599 to 157,999
GFF1486
Position (kb)
155
156 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 154.111 kb on - strand, within GFF1482 at 154.124 kb on - strand, within GFF1482 at 154.226 kb on + strand at 154.451 kb on - strand at 154.472 kb on + strand at 154.473 kb on - strand at 154.574 kb on + strand, within GFF1483 at 154.575 kb on - strand, within GFF1483 at 154.627 kb on + strand, within GFF1483 at 154.627 kb on + strand, within GFF1483 at 154.628 kb on - strand, within GFF1483 at 154.631 kb on + strand, within GFF1483 at 154.632 kb on - strand, within GFF1483 at 154.635 kb on - strand, within GFF1483 at 154.651 kb on + strand, within GFF1483 at 154.746 kb on + strand, within GFF1483 at 154.938 kb on - strand, within GFF1483 at 155.000 kb on - strand, within GFF1483 at 155.002 kb on + strand, within GFF1483 at 155.108 kb on + strand at 155.437 kb on + strand, within GFF1484 at 155.493 kb on + strand, within GFF1484 at 155.494 kb on - strand, within GFF1484 at 155.512 kb on + strand, within GFF1484 at 155.625 kb on + strand, within GFF1484 at 155.626 kb on - strand, within GFF1484 at 155.628 kb on - strand, within GFF1484 at 155.675 kb on + strand, within GFF1484 at 155.676 kb on - strand, within GFF1484 at 155.676 kb on - strand, within GFF1484 at 155.676 kb on - strand, within GFF1484 at 155.861 kb on + strand at 155.870 kb on + strand at 155.870 kb on + strand at 155.870 kb on + strand at 155.871 kb on - strand at 155.915 kb on + strand at 155.921 kb on + strand at 155.968 kb on - strand at 156.057 kb on - strand, within GFF1485 at 156.104 kb on + strand, within GFF1485 at 156.104 kb on + strand, within GFF1485 at 156.104 kb on + strand, within GFF1485 at 156.105 kb on - strand, within GFF1485 at 156.105 kb on - strand, within GFF1485 at 156.172 kb on - strand, within GFF1485 at 156.247 kb on + strand, within GFF1485 at 156.295 kb on + strand, within GFF1485 at 156.385 kb on - strand, within GFF1485 at 156.407 kb on + strand, within GFF1485 at 156.518 kb on + strand at 156.708 kb on - strand at 156.714 kb on - strand at 156.714 kb on - strand at 156.795 kb on + strand, within GFF1486 at 156.795 kb on + strand, within GFF1486 at 156.796 kb on - strand, within GFF1486 at 156.796 kb on - strand, within GFF1486 at 156.816 kb on + strand, within GFF1486 at 156.833 kb on - strand, within GFF1486
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 154,111 - GFF1482 0.86 +1.4 154,124 - GFF1482 0.87 -0.6 154,226 + +0.5 154,451 - +0.1 154,472 + +0.4 154,473 - +0.2 154,574 + GFF1483 0.19 -0.5 154,575 - GFF1483 0.19 +0.3 154,627 + GFF1483 0.28 -1.9 154,627 + GFF1483 0.28 +0.7 154,628 - GFF1483 0.28 -0.0 154,631 + GFF1483 0.28 +0.5 154,632 - GFF1483 0.28 -1.4 154,635 - GFF1483 0.29 +0.4 154,651 + GFF1483 0.31 -1.3 154,746 + GFF1483 0.47 +0.7 154,938 - GFF1483 0.77 -0.5 155,000 - GFF1483 0.87 +0.1 155,002 + GFF1483 0.87 -0.2 155,108 + -0.7 155,437 + GFF1484 0.47 +0.1 155,493 + GFF1484 0.54 +0.1 155,494 - GFF1484 0.54 -0.2 155,512 + GFF1484 0.57 -0.2 155,625 + GFF1484 0.71 -1.6 155,626 - GFF1484 0.71 +0.4 155,628 - GFF1484 0.72 +0.8 155,675 + GFF1484 0.78 -1.6 155,676 - GFF1484 0.78 -1.3 155,676 - GFF1484 0.78 +0.1 155,676 - GFF1484 0.78 -0.2 155,861 + +0.2 155,870 + -0.3 155,870 + +0.6 155,870 + -0.1 155,871 - +0.0 155,915 + -0.7 155,921 + +0.1 155,968 - +0.7 156,057 - GFF1485 0.23 -3.2 156,104 + GFF1485 0.31 -0.6 156,104 + GFF1485 0.31 -0.2 156,104 + GFF1485 0.31 +0.1 156,105 - GFF1485 0.31 -0.8 156,105 - GFF1485 0.31 -0.1 156,172 - GFF1485 0.42 -0.3 156,247 + GFF1485 0.55 -1.0 156,295 + GFF1485 0.63 -0.8 156,385 - GFF1485 0.78 -1.2 156,407 + GFF1485 0.82 -0.7 156,518 + -0.0 156,708 - -0.2 156,714 - +0.2 156,714 - -0.5 156,795 + GFF1486 0.14 -1.1 156,795 + GFF1486 0.14 +0.3 156,796 - GFF1486 0.14 +0.3 156,796 - GFF1486 0.14 -1.7 156,816 + GFF1486 0.15 +0.0 156,833 - GFF1486 0.17 -0.4
Or see this region's nucleotide sequence