Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1431

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1429 and GFF1430 are separated by 247 nucleotidesGFF1430 and GFF1431 are separated by 209 nucleotidesGFF1431 and GFF1432 are separated by 25 nucleotidesGFF1432 and GFF1433 are separated by 509 nucleotides GFF1429 - Excinuclease ABC subunit A, at 73,344 to 76,169 GFF1429 GFF1430 - Single-stranded DNA-binding protein, at 76,417 to 76,947 GFF1430 GFF1431 - FIG01048353: hypothetical protein, at 77,157 to 77,312 GFF1431 GFF1432 - hypothetical protein, at 77,338 to 77,478 GFF1432 GFF1433 - Putative inner membrane or exported protein, at 77,988 to 78,620 GFF1433 Position (kb) 77 78Strain fitness (log2 ratio) -2 -1 0 1at 76.236 kb on + strandat 76.303 kb on + strandat 76.346 kb on + strandat 77.042 kb on - strandat 78.271 kb on + strand, within GFF1433at 78.305 kb on - strand, within GFF1433

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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76,236 + +0.5
76,303 + -0.4
76,346 + +0.9
77,042 - +0.3
78,271 + GFF1433 0.45 -2.0
78,305 - GFF1433 0.50 +0.5

Or see this region's nucleotide sequence