Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1421

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1420 and GFF1421 are separated by 64 nucleotidesGFF1421 and GFF1422 are separated by 31 nucleotides GFF1420 - Quinone oxidoreductase (EC 1.6.5.5), at 65,674 to 66,657 GFF1420 GFF1421 - Replicative DNA helicase (EC 3.6.1.-), at 66,722 to 68,137 GFF1421 GFF1422 - Alanine racemase (EC 5.1.1.1), at 68,169 to 69,248 GFF1422 Position (kb) 66 67 68 69Strain fitness (log2 ratio) -2 -1 0 1 2at 65.753 kb on + strandat 65.848 kb on - strand, within GFF1420at 65.850 kb on + strand, within GFF1420at 65.850 kb on + strand, within GFF1420at 65.851 kb on - strand, within GFF1420at 65.852 kb on + strand, within GFF1420at 65.853 kb on - strand, within GFF1420at 66.018 kb on - strand, within GFF1420at 66.088 kb on - strand, within GFF1420at 66.251 kb on + strand, within GFF1420at 66.251 kb on + strand, within GFF1420at 66.251 kb on + strand, within GFF1420at 66.374 kb on + strand, within GFF1420at 66.521 kb on + strand, within GFF1420at 66.521 kb on + strand, within GFF1420at 66.522 kb on - strand, within GFF1420at 66.708 kb on + strandat 68.109 kb on - strandat 68.124 kb on - strandat 68.135 kb on + strandat 68.136 kb on - strandat 68.249 kb on + strandat 68.359 kb on + strand, within GFF1422at 68.457 kb on - strand, within GFF1422at 68.465 kb on - strand, within GFF1422at 68.465 kb on - strand, within GFF1422at 68.589 kb on + strand, within GFF1422at 68.633 kb on + strand, within GFF1422at 68.721 kb on - strand, within GFF1422at 68.721 kb on - strand, within GFF1422at 68.738 kb on + strand, within GFF1422

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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65,753 + +0.7
65,848 - GFF1420 0.18 -0.4
65,850 + GFF1420 0.18 +0.6
65,850 + GFF1420 0.18 +0.7
65,851 - GFF1420 0.18 +0.7
65,852 + GFF1420 0.18 +1.3
65,853 - GFF1420 0.18 +1.7
66,018 - GFF1420 0.35 -1.9
66,088 - GFF1420 0.42 +0.8
66,251 + GFF1420 0.59 +0.3
66,251 + GFF1420 0.59 +0.5
66,251 + GFF1420 0.59 -1.7
66,374 + GFF1420 0.71 -0.4
66,521 + GFF1420 0.86 -0.9
66,521 + GFF1420 0.86 +0.3
66,522 - GFF1420 0.86 +0.3
66,708 + +0.7
68,109 - +2.4
68,124 - +0.0
68,135 + +0.1
68,136 - -1.0
68,249 + +0.4
68,359 + GFF1422 0.18 -0.3
68,457 - GFF1422 0.27 +0.4
68,465 - GFF1422 0.27 +0.0
68,465 - GFF1422 0.27 +0.4
68,589 + GFF1422 0.39 +0.1
68,633 + GFF1422 0.43 -0.4
68,721 - GFF1422 0.51 +0.8
68,721 - GFF1422 0.51 -0.1
68,738 + GFF1422 0.53 +0.8

Or see this region's nucleotide sequence