Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1395 and GFF1396 are separated by 344 nucleotides GFF1396 and GFF1397 are separated by 440 nucleotides GFF1397 and GFF1398 are separated by 63 nucleotides
GFF1395 - Aspartokinase (EC 2.7.2.4), at 38,598 to 39,947
GFF1395
GFF1396 - Glucose-6-phosphate isomerase (EC 5.3.1.9), at 40,292 to 41,941
GFF1396
GFF1397 - YjbE secreted protein, at 42,382 to 42,627
GFF1397
GFF1398 - YjbF outer membrane lipoprotein, at 42,691 to 43,329
GFF1398
Position (kb) 40
41
42 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3 at 39.339 kb on - strand, within GFF1395 at 39.339 kb on - strand, within GFF1395 at 39.339 kb on - strand, within GFF1395 at 39.408 kb on - strand, within GFF1395 at 39.543 kb on + strand, within GFF1395 at 39.543 kb on + strand, within GFF1395 at 39.573 kb on + strand, within GFF1395 at 39.867 kb on + strand at 39.867 kb on + strand at 39.867 kb on + strand at 39.868 kb on - strand at 39.948 kb on - strand at 39.948 kb on - strand at 40.028 kb on - strand at 40.042 kb on + strand at 40.117 kb on + strand at 40.119 kb on + strand at 40.119 kb on + strand at 40.119 kb on + strand at 40.165 kb on + strand at 40.166 kb on - strand at 40.171 kb on - strand at 40.175 kb on - strand at 40.175 kb on - strand at 40.215 kb on + strand at 40.610 kb on - strand, within GFF1396 at 40.807 kb on + strand, within GFF1396 at 41.068 kb on + strand, within GFF1396 at 41.068 kb on + strand, within GFF1396 at 41.069 kb on - strand, within GFF1396 at 41.312 kb on + strand, within GFF1396 at 41.314 kb on + strand, within GFF1396 at 41.314 kb on + strand, within GFF1396 at 41.314 kb on + strand, within GFF1396 at 41.315 kb on - strand, within GFF1396 at 41.315 kb on - strand, within GFF1396 at 41.315 kb on - strand, within GFF1396 at 41.315 kb on - strand, within GFF1396 at 41.315 kb on - strand, within GFF1396 at 41.315 kb on - strand, within GFF1396 at 41.347 kb on + strand, within GFF1396 at 41.348 kb on - strand, within GFF1396 at 41.369 kb on + strand, within GFF1396 at 41.369 kb on + strand, within GFF1396 at 41.370 kb on - strand, within GFF1396 at 41.416 kb on + strand, within GFF1396 at 41.477 kb on - strand, within GFF1396 at 41.705 kb on - strand, within GFF1396 at 41.756 kb on + strand, within GFF1396 at 41.756 kb on + strand, within GFF1396 at 41.757 kb on - strand, within GFF1396 at 41.757 kb on - strand, within GFF1396 at 41.801 kb on - strand at 41.893 kb on + strand at 41.894 kb on - strand at 41.894 kb on - strand at 41.899 kb on + strand at 41.921 kb on + strand at 41.964 kb on - strand at 41.967 kb on + strand at 41.968 kb on - strand at 41.968 kb on - strand at 42.027 kb on - strand at 42.032 kb on - strand at 42.400 kb on + strand at 42.533 kb on + strand, within GFF1397 at 42.533 kb on + strand, within GFF1397 at 42.534 kb on - strand, within GFF1397 at 42.641 kb on - strand at 42.725 kb on + strand at 42.835 kb on + strand, within GFF1398
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 39,339 - GFF1395 0.55 +1.1 39,339 - GFF1395 0.55 +3.0 39,339 - GFF1395 0.55 -0.1 39,408 - GFF1395 0.60 -0.1 39,543 + GFF1395 0.70 -0.2 39,543 + GFF1395 0.70 -1.6 39,573 + GFF1395 0.72 -0.5 39,867 + +0.9 39,867 + -0.8 39,867 + -1.6 39,868 - +0.2 39,948 - -0.1 39,948 - -0.7 40,028 - +1.2 40,042 + -1.0 40,117 + +0.7 40,119 + +0.3 40,119 + +0.6 40,119 + -0.2 40,165 + +1.2 40,166 - -0.1 40,171 - +0.8 40,175 - -0.4 40,175 - +1.4 40,215 + +0.6 40,610 - GFF1396 0.19 -4.2 40,807 + GFF1396 0.31 -0.3 41,068 + GFF1396 0.47 -4.2 41,068 + GFF1396 0.47 -5.0 41,069 - GFF1396 0.47 -3.9 41,312 + GFF1396 0.62 -0.6 41,314 + GFF1396 0.62 -1.2 41,314 + GFF1396 0.62 -2.9 41,314 + GFF1396 0.62 -2.9 41,315 - GFF1396 0.62 -4.3 41,315 - GFF1396 0.62 +0.7 41,315 - GFF1396 0.62 -3.6 41,315 - GFF1396 0.62 -3.9 41,315 - GFF1396 0.62 -3.0 41,315 - GFF1396 0.62 -4.5 41,347 + GFF1396 0.64 -1.4 41,348 - GFF1396 0.64 -3.4 41,369 + GFF1396 0.65 -2.6 41,369 + GFF1396 0.65 -1.4 41,370 - GFF1396 0.65 -3.6 41,416 + GFF1396 0.68 -2.9 41,477 - GFF1396 0.72 +1.0 41,705 - GFF1396 0.86 -5.4 41,756 + GFF1396 0.89 -2.7 41,756 + GFF1396 0.89 -0.9 41,757 - GFF1396 0.89 -2.4 41,757 - GFF1396 0.89 -6.1 41,801 - -1.9 41,893 + -3.4 41,894 - -5.6 41,894 - -2.3 41,899 + -2.8 41,921 + -1.5 41,964 - -0.5 41,967 + -0.1 41,968 - -0.1 41,968 - +0.4 42,027 - -0.9 42,032 - +0.5 42,400 + +0.4 42,533 + GFF1397 0.61 +0.1 42,533 + GFF1397 0.61 -1.5 42,534 - GFF1397 0.62 -0.5 42,641 - -0.2 42,725 + +0.8 42,835 + GFF1398 0.23 +0.8
Or see this region's nucleotide sequence