Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1358 and GFF1359 are separated by 266 nucleotides GFF1359 and GFF1360 are separated by 75 nucleotides GFF1360 and GFF1361 are separated by 207 nucleotides
GFF1358 - 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13), at 8,467 to 12,150
GFF1358
GFF1359 - Sodium-dependent phosphate transporter, at 12,417 to 14,048
GFF1359
GFF1360 - Alpha-aspartyl dipeptidase Peptidase E (EC 3.4.13.21), at 14,124 to 14,813
GFF1360
GFF1361 - Nucleoprotein/polynucleotide-associated enzyme, at 15,021 to 15,560
GFF1361
Position (kb) 12
13
14
15 Strain fitness (log2 ratio) -1
0
1
2 at 11.716 kb on - strand, within GFF1358 at 11.716 kb on - strand, within GFF1358 at 11.770 kb on + strand, within GFF1358 at 11.771 kb on - strand, within GFF1358 at 11.787 kb on - strand at 11.916 kb on + strand at 11.927 kb on + strand at 12.073 kb on + strand at 12.181 kb on + strand at 12.181 kb on + strand at 12.181 kb on + strand at 12.181 kb on + strand at 12.182 kb on - strand at 12.182 kb on - strand at 12.215 kb on + strand at 12.216 kb on - strand at 12.230 kb on - strand at 12.232 kb on - strand at 12.297 kb on - strand at 12.297 kb on - strand at 12.299 kb on + strand at 12.299 kb on + strand at 12.303 kb on + strand at 12.391 kb on + strand at 12.394 kb on - strand at 12.473 kb on + strand at 12.474 kb on - strand at 12.474 kb on - strand at 12.701 kb on - strand, within GFF1359 at 12.782 kb on + strand, within GFF1359 at 12.930 kb on - strand, within GFF1359 at 12.966 kb on - strand, within GFF1359 at 13.025 kb on + strand, within GFF1359 at 13.025 kb on + strand, within GFF1359 at 13.027 kb on + strand, within GFF1359 at 13.028 kb on - strand, within GFF1359 at 13.145 kb on - strand, within GFF1359 at 13.145 kb on - strand, within GFF1359 at 13.261 kb on - strand, within GFF1359 at 13.566 kb on + strand, within GFF1359 at 13.775 kb on + strand, within GFF1359 at 13.865 kb on + strand, within GFF1359 at 13.973 kb on + strand at 13.973 kb on + strand at 13.998 kb on + strand at 14.000 kb on + strand at 14.043 kb on + strand at 14.047 kb on - strand at 14.112 kb on + strand at 14.291 kb on + strand, within GFF1360 at 14.336 kb on + strand, within GFF1360 at 14.467 kb on + strand, within GFF1360 at 14.467 kb on + strand, within GFF1360 at 14.485 kb on - strand, within GFF1360 at 14.535 kb on + strand, within GFF1360 at 14.705 kb on + strand, within GFF1360 at 14.815 kb on - strand at 14.850 kb on + strand at 14.851 kb on - strand at 14.870 kb on + strand at 14.878 kb on + strand at 14.900 kb on - strand at 14.997 kb on + strand at 15.001 kb on + strand at 15.038 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 11,716 - GFF1358 0.88 +0.2 11,716 - GFF1358 0.88 +1.0 11,770 + GFF1358 0.90 +0.1 11,771 - GFF1358 0.90 +1.7 11,787 - +0.6 11,916 + -0.9 11,927 + +0.9 12,073 + -0.4 12,181 + +0.3 12,181 + +0.4 12,181 + +0.2 12,181 + -1.7 12,182 - +0.3 12,182 - -1.4 12,215 + +0.4 12,216 - -0.3 12,230 - +0.2 12,232 - +0.5 12,297 - -0.2 12,297 - -0.1 12,299 + -0.1 12,299 + +0.6 12,303 + +0.9 12,391 + +0.3 12,394 - -1.2 12,473 + +0.0 12,474 - +0.1 12,474 - -0.2 12,701 - GFF1359 0.17 +0.2 12,782 + GFF1359 0.22 -0.6 12,930 - GFF1359 0.31 +0.4 12,966 - GFF1359 0.34 +0.6 13,025 + GFF1359 0.37 -1.6 13,025 + GFF1359 0.37 -1.2 13,027 + GFF1359 0.37 +0.5 13,028 - GFF1359 0.37 -0.5 13,145 - GFF1359 0.45 -0.2 13,145 - GFF1359 0.45 +0.4 13,261 - GFF1359 0.52 +0.4 13,566 + GFF1359 0.70 +0.5 13,775 + GFF1359 0.83 -1.2 13,865 + GFF1359 0.89 -0.0 13,973 + +0.8 13,973 + -0.6 13,998 + +0.5 14,000 + -0.2 14,043 + +0.4 14,047 - +0.6 14,112 + -0.4 14,291 + GFF1360 0.24 +0.5 14,336 + GFF1360 0.31 +1.2 14,467 + GFF1360 0.50 +0.8 14,467 + GFF1360 0.50 +0.1 14,485 - GFF1360 0.52 +0.5 14,535 + GFF1360 0.60 +0.3 14,705 + GFF1360 0.84 -1.3 14,815 - +1.3 14,850 + +0.8 14,851 - +0.1 14,870 + +0.5 14,878 + +0.2 14,900 - -0.2 14,997 + -0.2 15,001 + +2.3 15,038 - +0.1
Or see this region's nucleotide sequence