Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1319 and GFF1320 are separated by 468 nucleotides GFF1320 and GFF1321 overlap by 2 nucleotides GFF1321 and GFF1322 are separated by 13 nucleotides
GFF1319 - 2-acylglycerophosphoethanolamine acyltransferase (EC 2.3.1.40) / Acyl-[acyl-carrier-protein] synthetase (EC 6.2.1.20), at 26,680 to 28,839
GFF1319
GFF1320 - hypothetical protein, at 29,308 to 29,433
GFF1320
GFF1321 - Galactose operon repressor, GalR-LacI family of transcriptional regulators, at 29,432 to 30,460
GFF1321
GFF1322 - Galactose operon repressor, GalR-LacI family of transcriptional regulators, at 30,474 to 31,493
GFF1322
Position (kb) 29
30
31 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 28.579 kb on - strand, within GFF1319 at 28.641 kb on + strand at 28.643 kb on + strand at 28.665 kb on + strand at 28.668 kb on - strand at 28.668 kb on - strand at 28.668 kb on - strand at 28.668 kb on - strand at 28.743 kb on + strand at 28.744 kb on - strand at 28.747 kb on + strand at 28.890 kb on - strand at 29.095 kb on + strand at 29.096 kb on - strand at 29.167 kb on - strand at 29.201 kb on + strand at 29.202 kb on - strand at 29.228 kb on - strand at 29.228 kb on - strand at 29.228 kb on - strand at 29.228 kb on - strand at 29.422 kb on - strand at 29.425 kb on + strand at 29.425 kb on + strand at 29.425 kb on + strand at 29.425 kb on + strand at 29.425 kb on + strand at 29.426 kb on - strand at 29.426 kb on - strand at 29.429 kb on - strand at 29.443 kb on - strand at 29.452 kb on - strand at 29.498 kb on - strand at 29.723 kb on + strand, within GFF1321 at 29.883 kb on + strand, within GFF1321 at 29.973 kb on - strand, within GFF1321 at 29.975 kb on - strand, within GFF1321 at 30.161 kb on + strand, within GFF1321 at 30.161 kb on + strand, within GFF1321 at 30.195 kb on + strand, within GFF1321 at 30.196 kb on - strand, within GFF1321 at 30.205 kb on + strand, within GFF1321 at 30.232 kb on - strand, within GFF1321 at 30.255 kb on + strand, within GFF1321 at 30.343 kb on - strand, within GFF1321 at 30.375 kb on + strand at 30.384 kb on + strand at 30.384 kb on + strand at 30.384 kb on + strand at 30.384 kb on + strand at 30.467 kb on + strand at 30.467 kb on + strand at 30.468 kb on - strand at 30.770 kb on + strand, within GFF1322 at 31.023 kb on - strand, within GFF1322 at 31.023 kb on - strand, within GFF1322 at 31.037 kb on + strand, within GFF1322 at 31.060 kb on + strand, within GFF1322 at 31.132 kb on - strand, within GFF1322 at 31.198 kb on + strand, within GFF1322 at 31.199 kb on - strand, within GFF1322 at 31.208 kb on + strand, within GFF1322 at 31.258 kb on + strand, within GFF1322 at 31.259 kb on - strand, within GFF1322 at 31.345 kb on + strand, within GFF1322
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 28,579 - GFF1319 0.88 +0.9 28,641 + -1.1 28,643 + +0.1 28,665 + +1.0 28,668 - +0.1 28,668 - +1.4 28,668 - +0.5 28,668 - +1.9 28,743 + -2.7 28,744 - +0.8 28,747 + -0.6 28,890 - +0.1 29,095 + -0.4 29,096 - +0.5 29,167 - -0.3 29,201 + +0.6 29,202 - -3.1 29,228 - +0.9 29,228 - -1.4 29,228 - +0.5 29,228 - -0.2 29,422 - +0.9 29,425 + +0.2 29,425 + +0.4 29,425 + +1.4 29,425 + +1.2 29,425 + +0.8 29,426 - -0.9 29,426 - +1.6 29,429 - +0.3 29,443 - -0.9 29,452 - -0.6 29,498 - -1.9 29,723 + GFF1321 0.28 +1.2 29,883 + GFF1321 0.44 +2.1 29,973 - GFF1321 0.53 +1.7 29,975 - GFF1321 0.53 -2.3 30,161 + GFF1321 0.71 +0.2 30,161 + GFF1321 0.71 +1.1 30,195 + GFF1321 0.74 +0.5 30,196 - GFF1321 0.74 +1.1 30,205 + GFF1321 0.75 +1.6 30,232 - GFF1321 0.78 +1.0 30,255 + GFF1321 0.80 +0.1 30,343 - GFF1321 0.89 +1.2 30,375 + +1.2 30,384 + +0.2 30,384 + +0.2 30,384 + +0.2 30,384 + +2.6 30,467 + -0.2 30,467 + -1.0 30,468 - -1.1 30,770 + GFF1322 0.29 +0.8 31,023 - GFF1322 0.54 -0.1 31,023 - GFF1322 0.54 +1.9 31,037 + GFF1322 0.55 +1.4 31,060 + GFF1322 0.57 -1.0 31,132 - GFF1322 0.65 -0.2 31,198 + GFF1322 0.71 +0.3 31,199 - GFF1322 0.71 -0.0 31,208 + GFF1322 0.72 -0.6 31,258 + GFF1322 0.77 +0.6 31,259 - GFF1322 0.77 -0.6 31,345 + GFF1322 0.85 +0.2
Or see this region's nucleotide sequence