Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1310 and GFF1311 are separated by 215 nucleotides GFF1311 and GFF1312 are separated by 12 nucleotides
GFF1310 - Prolipoprotein diacylglyceryl transferase (EC 2.4.99.-), at 17,626 to 18,501
GFF1310
GFF1311 - FIG001592: Phosphocarrier protein kinase/phosphorylase, nitrogen regulation associated, at 18,717 to 20,963
GFF1311
GFF1312 - 'Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-)' transl_table=11, at 20,976 to 21,506
GFF1312
Position (kb) 18
19
20
21 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 18.549 kb on - strand at 18.589 kb on - strand at 18.592 kb on - strand at 18.635 kb on - strand at 18.638 kb on + strand at 18.638 kb on + strand at 18.638 kb on + strand at 18.638 kb on + strand at 18.638 kb on + strand at 18.638 kb on + strand at 18.639 kb on - strand at 18.639 kb on - strand at 18.718 kb on + strand at 18.718 kb on + strand at 19.046 kb on - strand, within GFF1311 at 19.122 kb on - strand, within GFF1311 at 19.158 kb on + strand, within GFF1311 at 19.182 kb on - strand, within GFF1311 at 19.280 kb on + strand, within GFF1311 at 19.433 kb on + strand, within GFF1311 at 19.433 kb on + strand, within GFF1311 at 19.435 kb on + strand, within GFF1311 at 19.435 kb on + strand, within GFF1311 at 19.435 kb on + strand, within GFF1311 at 19.436 kb on - strand, within GFF1311 at 19.725 kb on + strand, within GFF1311 at 19.726 kb on - strand, within GFF1311 at 19.760 kb on + strand, within GFF1311 at 19.785 kb on + strand, within GFF1311 at 19.818 kb on - strand, within GFF1311 at 19.827 kb on + strand, within GFF1311 at 19.828 kb on - strand, within GFF1311 at 20.071 kb on - strand, within GFF1311 at 20.231 kb on - strand, within GFF1311 at 20.231 kb on - strand, within GFF1311 at 20.351 kb on + strand, within GFF1311 at 20.352 kb on - strand, within GFF1311 at 20.672 kb on - strand, within GFF1311 at 20.675 kb on + strand, within GFF1311 at 20.675 kb on + strand, within GFF1311 at 20.675 kb on + strand, within GFF1311 at 20.813 kb on - strand at 20.813 kb on - strand at 20.861 kb on + strand at 20.940 kb on + strand at 20.963 kb on + strand at 21.027 kb on - strand at 21.090 kb on - strand, within GFF1312 at 21.156 kb on - strand, within GFF1312 at 21.195 kb on + strand, within GFF1312 at 21.328 kb on - strand, within GFF1312 at 21.465 kb on + strand at 21.474 kb on + strand at 21.474 kb on + strand at 21.475 kb on - strand at 21.512 kb on + strand at 21.523 kb on - strand at 21.529 kb on + strand at 21.530 kb on - strand at 21.530 kb on - strand at 21.530 kb on - strand at 21.530 kb on - strand at 21.532 kb on - strand at 21.532 kb on - strand at 21.532 kb on - strand at 21.533 kb on + strand at 21.534 kb on - strand at 21.534 kb on - strand at 21.550 kb on + strand at 21.582 kb on - strand at 21.802 kb on + strand at 21.803 kb on - strand at 21.946 kb on + strand at 21.947 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 18,549 - -0.3 18,589 - -1.1 18,592 - -1.0 18,635 - -0.4 18,638 + -1.2 18,638 + -1.2 18,638 + -0.5 18,638 + -1.9 18,638 + -1.8 18,638 + -0.2 18,639 - -0.3 18,639 - +0.7 18,718 + -0.5 18,718 + -1.4 19,046 - GFF1311 0.15 -3.8 19,122 - GFF1311 0.18 -2.8 19,158 + GFF1311 0.20 -2.0 19,182 - GFF1311 0.21 -3.1 19,280 + GFF1311 0.25 -2.3 19,433 + GFF1311 0.32 -0.5 19,433 + GFF1311 0.32 -4.1 19,435 + GFF1311 0.32 -2.3 19,435 + GFF1311 0.32 -1.3 19,435 + GFF1311 0.32 -3.2 19,436 - GFF1311 0.32 -1.9 19,725 + GFF1311 0.45 -1.2 19,726 - GFF1311 0.45 -2.1 19,760 + GFF1311 0.46 -3.1 19,785 + GFF1311 0.48 -1.6 19,818 - GFF1311 0.49 -1.7 19,827 + GFF1311 0.49 -3.2 19,828 - GFF1311 0.49 -4.9 20,071 - GFF1311 0.60 -1.6 20,231 - GFF1311 0.67 -4.7 20,231 - GFF1311 0.67 -2.3 20,351 + GFF1311 0.73 -4.1 20,352 - GFF1311 0.73 -2.5 20,672 - GFF1311 0.87 -4.0 20,675 + GFF1311 0.87 -3.8 20,675 + GFF1311 0.87 -2.5 20,675 + GFF1311 0.87 -3.2 20,813 - -3.1 20,813 - -2.8 20,861 + -2.3 20,940 + -5.6 20,963 + -2.6 21,027 - +2.4 21,090 - GFF1312 0.21 +3.3 21,156 - GFF1312 0.34 +3.6 21,195 + GFF1312 0.41 -0.2 21,328 - GFF1312 0.66 +2.4 21,465 + +2.3 21,474 + -0.1 21,474 + -0.4 21,475 - +2.1 21,512 + -2.4 21,523 - -1.7 21,529 + +1.1 21,530 - +1.2 21,530 - +1.8 21,530 - +1.1 21,530 - -1.8 21,532 - +0.1 21,532 - +1.2 21,532 - +1.2 21,533 + -3.8 21,534 - +0.4 21,534 - +0.3 21,550 + +0.8 21,582 - +1.3 21,802 + +2.8 21,803 - +1.0 21,946 + +0.2 21,947 - +0.4
Or see this region's nucleotide sequence