Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1307 and GFF1308 are separated by 48 nucleotides GFF1308 and GFF1309 overlap by 8 nucleotides GFF1309 and GFF1310 are separated by 6 nucleotides GFF1310 and GFF1311 are separated by 215 nucleotides
GFF1307 - Prepilin peptidase dependent protein A precursor, at 16,170 to 16,640
GFF1307
GFF1308 - FIG102772: hypothetical protein, at 16,689 to 16,832
GFF1308
GFF1309 - Thymidylate synthase (EC 2.1.1.45), at 16,825 to 17,619
GFF1309
GFF1310 - Prolipoprotein diacylglyceryl transferase (EC 2.4.99.-), at 17,626 to 18,501
GFF1310
GFF1311 - FIG001592: Phosphocarrier protein kinase/phosphorylase, nitrogen regulation associated, at 18,717 to 20,963
GFF1311
Position (kb) 17
18
19 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 18.549 kb on - strand at 18.589 kb on - strand at 18.592 kb on - strand at 18.635 kb on - strand at 18.638 kb on + strand at 18.638 kb on + strand at 18.638 kb on + strand at 18.638 kb on + strand at 18.638 kb on + strand at 18.638 kb on + strand at 18.639 kb on - strand at 18.639 kb on - strand at 18.718 kb on + strand at 18.718 kb on + strand at 19.046 kb on - strand, within GFF1311 at 19.122 kb on - strand, within GFF1311 at 19.158 kb on + strand, within GFF1311 at 19.182 kb on - strand, within GFF1311 at 19.280 kb on + strand, within GFF1311 at 19.433 kb on + strand, within GFF1311 at 19.433 kb on + strand, within GFF1311 at 19.435 kb on + strand, within GFF1311 at 19.435 kb on + strand, within GFF1311 at 19.435 kb on + strand, within GFF1311 at 19.436 kb on - strand, within GFF1311
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 18,549 - -0.3 18,589 - -1.1 18,592 - -1.0 18,635 - -0.4 18,638 + -1.2 18,638 + -1.2 18,638 + -0.5 18,638 + -1.9 18,638 + -1.8 18,638 + -0.2 18,639 - -0.3 18,639 - +0.7 18,718 + -0.5 18,718 + -1.4 19,046 - GFF1311 0.15 -3.8 19,122 - GFF1311 0.18 -2.8 19,158 + GFF1311 0.20 -2.0 19,182 - GFF1311 0.21 -3.1 19,280 + GFF1311 0.25 -2.3 19,433 + GFF1311 0.32 -0.5 19,433 + GFF1311 0.32 -4.1 19,435 + GFF1311 0.32 -2.3 19,435 + GFF1311 0.32 -1.3 19,435 + GFF1311 0.32 -3.2 19,436 - GFF1311 0.32 -1.9
Or see this region's nucleotide sequence