Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1309

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1306 and GFF1307 are separated by 29 nucleotidesGFF1307 and GFF1308 are separated by 48 nucleotidesGFF1308 and GFF1309 overlap by 8 nucleotidesGFF1309 and GFF1310 are separated by 6 nucleotides GFF1306 - FIG004819: Prepilin peptidase dependent protein B precursor, at 15,616 to 16,140 GFF1306 GFF1307 - Prepilin peptidase dependent protein A precursor, at 16,170 to 16,640 GFF1307 GFF1308 - FIG102772: hypothetical protein, at 16,689 to 16,832 GFF1308 GFF1309 - Thymidylate synthase (EC 2.1.1.45), at 16,825 to 17,619 GFF1309 GFF1310 - Prolipoprotein diacylglyceryl transferase (EC 2.4.99.-), at 17,626 to 18,501 GFF1310 Position (kb) 16 17 18Strain fitness (log2 ratio) -1 0 1 2at 15.882 kb on + strand, within GFF1306at 15.901 kb on - strand, within GFF1306at 16.012 kb on - strand, within GFF1306at 16.013 kb on + strand, within GFF1306at 16.013 kb on + strand, within GFF1306at 16.014 kb on - strand, within GFF1306at 16.014 kb on - strand, within GFF1306at 16.078 kb on + strand, within GFF1306at 16.323 kb on - strand, within GFF1307at 16.395 kb on - strand, within GFF1307at 16.619 kb on + strandat 18.549 kb on - strandat 18.589 kb on - strandat 18.592 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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15,882 + GFF1306 0.51 +0.5
15,901 - GFF1306 0.54 +0.0
16,012 - GFF1306 0.75 -1.6
16,013 + GFF1306 0.76 -0.0
16,013 + GFF1306 0.76 -1.1
16,014 - GFF1306 0.76 +0.6
16,014 - GFF1306 0.76 -1.4
16,078 + GFF1306 0.88 +0.9
16,323 - GFF1307 0.32 +1.8
16,395 - GFF1307 0.48 -0.1
16,619 + +0.2
18,549 - -0.3
18,589 - -1.1
18,592 - -1.0

Or see this region's nucleotide sequence