Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1250 and GFF1251 overlap by 17 nucleotides GFF1251 and GFF1252 are separated by 11 nucleotides GFF1252 and GFF1253 are separated by 52 nucleotides GFF1253 and GFF1254 are separated by 56 nucleotides
GFF1250 - Alpha-helical coiled coil protein, at 79,887 to 81,002
GFF1250
GFF1251 - FIG01045535: hypothetical protein, at 80,986 to 81,504
GFF1251
GFF1252 - hypothetical protein, at 81,516 to 81,635
GFF1252
GFF1253 - FIG01047572: hypothetical protein, at 81,688 to 82,023
GFF1253
GFF1254 - FIG01046430: hypothetical protein, at 82,080 to 82,646
GFF1254
Position (kb) 81
82 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 80.819 kb on - strand, within GFF1250 at 81.048 kb on + strand, within GFF1251 at 81.124 kb on + strand, within GFF1251 at 81.125 kb on - strand, within GFF1251 at 81.288 kb on + strand, within GFF1251 at 81.289 kb on - strand, within GFF1251 at 81.289 kb on - strand, within GFF1251 at 81.395 kb on + strand, within GFF1251 at 81.569 kb on + strand, within GFF1252 at 81.569 kb on + strand, within GFF1252 at 81.672 kb on - strand at 81.697 kb on - strand at 81.759 kb on + strand, within GFF1253 at 81.794 kb on + strand, within GFF1253 at 81.838 kb on - strand, within GFF1253 at 81.843 kb on + strand, within GFF1253 at 81.859 kb on + strand, within GFF1253 at 81.886 kb on - strand, within GFF1253 at 81.941 kb on - strand, within GFF1253 at 81.941 kb on - strand, within GFF1253 at 81.941 kb on - strand, within GFF1253 at 82.012 kb on - strand at 82.012 kb on - strand at 82.035 kb on + strand at 82.049 kb on + strand at 82.049 kb on + strand at 82.050 kb on - strand at 82.317 kb on + strand, within GFF1254 at 82.377 kb on + strand, within GFF1254 at 82.377 kb on + strand, within GFF1254 at 82.377 kb on + strand, within GFF1254 at 82.377 kb on + strand, within GFF1254 at 82.377 kb on + strand, within GFF1254 at 82.378 kb on - strand, within GFF1254 at 82.378 kb on - strand, within GFF1254 at 82.414 kb on - strand, within GFF1254 at 82.485 kb on + strand, within GFF1254 at 82.517 kb on + strand, within GFF1254 at 82.537 kb on + strand, within GFF1254
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 80,819 - GFF1250 0.84 -0.5 81,048 + GFF1251 0.12 -1.1 81,124 + GFF1251 0.27 -0.9 81,125 - GFF1251 0.27 +1.9 81,288 + GFF1251 0.58 -0.1 81,289 - GFF1251 0.58 +0.0 81,289 - GFF1251 0.58 +0.4 81,395 + GFF1251 0.79 -0.2 81,569 + GFF1252 0.44 -1.6 81,569 + GFF1252 0.44 +0.0 81,672 - -0.5 81,697 - -0.1 81,759 + GFF1253 0.21 +1.5 81,794 + GFF1253 0.32 -1.8 81,838 - GFF1253 0.45 -0.5 81,843 + GFF1253 0.46 -0.7 81,859 + GFF1253 0.51 -0.6 81,886 - GFF1253 0.59 +0.8 81,941 - GFF1253 0.75 +0.2 81,941 - GFF1253 0.75 -0.5 81,941 - GFF1253 0.75 -0.1 82,012 - +0.3 82,012 - +0.3 82,035 + -0.3 82,049 + -0.7 82,049 + -2.9 82,050 - -0.9 82,317 + GFF1254 0.42 +0.8 82,377 + GFF1254 0.52 -0.8 82,377 + GFF1254 0.52 +0.1 82,377 + GFF1254 0.52 +0.1 82,377 + GFF1254 0.52 +1.6 82,377 + GFF1254 0.52 +0.3 82,378 - GFF1254 0.53 +0.2 82,378 - GFF1254 0.53 +0.5 82,414 - GFF1254 0.59 +0.3 82,485 + GFF1254 0.71 +0.4 82,517 + GFF1254 0.77 +0.0 82,537 + GFF1254 0.81 +0.2
Or see this region's nucleotide sequence