Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1190 and GFF1191 are separated by 114 nucleotides GFF1191 and GFF1192 are separated by 51 nucleotides GFF1192 and GFF1193 are separated by 429 nucleotides
GFF1190 - IncF plasmid conjugative transfer fertility inhibition protein FinO, at 39,639 to 40,202
GFF1190
GFF1191 - putative cytoplasmic protein, at 40,317 to 40,601
GFF1191
GFF1192 - putative cytoplasmic protein, at 40,653 to 40,769
GFF1192
GFF1193 - Endonuclease (EC 3.1.-.-), at 41,199 to 41,378
GFF1193
Position (kb) 40
41 Strain fitness (log2 ratio) -2
-1
0
1
2 at 39.723 kb on - strand, within GFF1190 at 39.723 kb on - strand, within GFF1190 at 39.997 kb on + strand, within GFF1190 at 40.035 kb on + strand, within GFF1190 at 40.035 kb on + strand, within GFF1190 at 40.069 kb on - strand, within GFF1190 at 40.084 kb on - strand, within GFF1190 at 40.084 kb on - strand, within GFF1190 at 40.097 kb on + strand, within GFF1190 at 40.097 kb on + strand, within GFF1190 at 40.097 kb on + strand, within GFF1190 at 40.097 kb on + strand, within GFF1190 at 40.100 kb on + strand, within GFF1190 at 40.120 kb on - strand, within GFF1190 at 40.218 kb on + strand at 40.219 kb on - strand at 40.219 kb on - strand at 40.220 kb on + strand at 40.220 kb on + strand at 40.314 kb on + strand at 40.322 kb on + strand at 40.322 kb on + strand at 40.323 kb on - strand at 40.323 kb on - strand at 40.323 kb on - strand at 40.342 kb on + strand at 40.455 kb on + strand, within GFF1191 at 40.455 kb on + strand, within GFF1191 at 40.552 kb on + strand, within GFF1191 at 40.552 kb on + strand, within GFF1191 at 40.580 kb on + strand at 40.581 kb on - strand at 40.613 kb on - strand at 40.743 kb on - strand, within GFF1192 at 40.861 kb on + strand at 40.866 kb on + strand at 40.866 kb on + strand at 40.867 kb on - strand at 40.876 kb on + strand at 40.878 kb on + strand at 40.889 kb on + strand at 40.889 kb on + strand at 40.966 kb on - strand at 40.966 kb on - strand at 41.051 kb on + strand at 41.051 kb on + strand at 41.051 kb on + strand at 41.052 kb on - strand at 41.052 kb on - strand at 41.070 kb on + strand at 41.105 kb on + strand at 41.105 kb on + strand at 41.106 kb on - strand at 41.109 kb on - strand at 41.248 kb on - strand, within GFF1193 at 41.309 kb on + strand, within GFF1193 at 41.313 kb on + strand, within GFF1193 at 41.313 kb on + strand, within GFF1193 at 41.376 kb on + strand at 41.387 kb on + strand at 41.388 kb on - strand at 41.441 kb on + strand at 41.441 kb on + strand at 41.442 kb on - strand at 41.495 kb on - strand at 41.507 kb on + strand at 41.534 kb on + strand at 41.600 kb on + strand at 41.642 kb on + strand at 41.642 kb on + strand at 41.727 kb on + strand at 41.727 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 39,723 - GFF1190 0.15 +0.2 39,723 - GFF1190 0.15 +0.2 39,997 + GFF1190 0.63 -2.7 40,035 + GFF1190 0.70 -1.5 40,035 + GFF1190 0.70 +0.5 40,069 - GFF1190 0.76 +0.1 40,084 - GFF1190 0.79 +0.5 40,084 - GFF1190 0.79 -1.8 40,097 + GFF1190 0.81 -0.2 40,097 + GFF1190 0.81 -0.5 40,097 + GFF1190 0.81 -0.3 40,097 + GFF1190 0.81 -0.7 40,100 + GFF1190 0.82 -0.7 40,120 - GFF1190 0.85 -0.6 40,218 + -0.1 40,219 - +1.5 40,219 - +0.3 40,220 + +1.3 40,220 + -1.1 40,314 + -0.0 40,322 + +0.4 40,322 + -0.0 40,323 - +0.4 40,323 - +0.1 40,323 - +0.4 40,342 + -0.0 40,455 + GFF1191 0.48 -1.5 40,455 + GFF1191 0.48 +0.7 40,552 + GFF1191 0.82 -1.2 40,552 + GFF1191 0.82 -1.0 40,580 + -0.5 40,581 - +0.5 40,613 - -0.1 40,743 - GFF1192 0.77 -1.2 40,861 + -1.3 40,866 + -2.3 40,866 + +1.3 40,867 - +1.6 40,876 + -2.5 40,878 + -0.1 40,889 + -0.6 40,889 + -0.1 40,966 - +0.9 40,966 - -2.0 41,051 + -0.7 41,051 + +1.6 41,051 + -0.1 41,052 - +0.3 41,052 - -1.2 41,070 + -0.5 41,105 + -1.0 41,105 + +0.2 41,106 - -0.2 41,109 - +0.4 41,248 - GFF1193 0.27 +0.4 41,309 + GFF1193 0.61 +0.1 41,313 + GFF1193 0.63 -1.5 41,313 + GFF1193 0.63 -0.7 41,376 + +2.1 41,387 + -0.4 41,388 - -2.2 41,441 + -0.2 41,441 + -0.2 41,442 - +0.3 41,495 - +2.1 41,507 + +2.4 41,534 + +0.6 41,600 + -0.4 41,642 + +0.8 41,642 + +0.7 41,727 + -0.1 41,727 + +0.2
Or see this region's nucleotide sequence