Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1127

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1126 and GFF1127 overlap by 1 nucleotidesGFF1127 and GFF1128 are separated by 154 nucleotidesGFF1128 and GFF1129 are separated by 2 nucleotides GFF1126 - Isoleucyl-tRNA synthetase (EC 6.1.1.5), at 314,603 to 317,473 GFF1126 GFF1127 - Lipoprotein signal peptidase (EC 3.4.23.36), at 317,473 to 317,973 GFF1127 GFF1128 - FKBP-type peptidyl-prolyl cis-trans isomerase SlpA (EC 5.2.1.8), at 318,128 to 318,577 GFF1128 GFF1129 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.1.2), at 318,580 to 319,530 GFF1129 Position (kb) 317 318Strain fitness (log2 ratio) -1 0 1 2at 317.971 kb on + strandat 317.971 kb on + strandat 317.977 kb on + strandat 317.978 kb on - strandat 317.981 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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317,971 + +0.9
317,971 + +1.0
317,977 + +1.5
317,978 - -0.7
317,981 + +2.2

Or see this region's nucleotide sequence