Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1029 and GFF1030 are separated by 388 nucleotides GFF1030 and GFF1138 are separated by 31 nucleotides GFF1138 and GFF1139 are separated by 31 nucleotides GFF1139 and GFF1140 are separated by 27 nucleotides GFF1140 and GFF1031 are separated by 190 nucleotides
GFF1029 - Putative membrane protein, at 212,398 to 213,324
GFF1029
GFF1030 - FIG00638078: hypothetical protein, at 213,713 to 213,949
GFF1030
GFF1138 - tRNA-Leu-CAG, at 213,981 to 214,067
GFF1138
GFF1139 - tRNA-Leu-CAG, at 214,099 to 214,185
GFF1139
GFF1140 - tRNA-Leu-CAG, at 214,213 to 214,299
GFF1140
GFF1031 - Ribosomal RNA small subunit methyltransferase C (EC 2.1.1.52), at 214,490 to 215,518
GFF1031
Position (kb) 213
214 Strain fitness (log2 ratio) -2
-1
0
1 at 212.751 kb on + strand, within GFF1029 at 212.752 kb on - strand, within GFF1029 at 212.752 kb on - strand, within GFF1029 at 212.752 kb on - strand, within GFF1029 at 212.752 kb on - strand, within GFF1029 at 212.752 kb on - strand, within GFF1029 at 212.771 kb on + strand, within GFF1029 at 212.807 kb on + strand, within GFF1029 at 212.807 kb on + strand, within GFF1029 at 212.808 kb on - strand, within GFF1029 at 212.834 kb on - strand, within GFF1029 at 212.838 kb on + strand, within GFF1029 at 212.870 kb on + strand, within GFF1029 at 212.870 kb on + strand, within GFF1029 at 212.870 kb on + strand, within GFF1029 at 212.870 kb on + strand, within GFF1029 at 212.871 kb on - strand, within GFF1029 at 212.871 kb on - strand, within GFF1029 at 212.896 kb on - strand, within GFF1029 at 212.963 kb on + strand, within GFF1029 at 213.086 kb on + strand, within GFF1029 at 213.145 kb on - strand, within GFF1029 at 213.255 kb on + strand at 213.287 kb on - strand at 213.432 kb on + strand at 213.600 kb on - strand at 213.748 kb on - strand, within GFF1030 at 213.750 kb on + strand, within GFF1030 at 213.896 kb on - strand, within GFF1030 at 213.973 kb on - strand at 213.978 kb on - strand at 214.369 kb on + strand at 214.374 kb on - strand at 214.479 kb on + strand at 214.480 kb on - strand at 214.525 kb on - strand at 214.772 kb on + strand, within GFF1031
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 212,751 + GFF1029 0.38 +0.4 212,752 - GFF1029 0.38 -0.2 212,752 - GFF1029 0.38 +0.7 212,752 - GFF1029 0.38 +0.6 212,752 - GFF1029 0.38 +1.1 212,752 - GFF1029 0.38 +0.0 212,771 + GFF1029 0.40 +1.0 212,807 + GFF1029 0.44 +0.4 212,807 + GFF1029 0.44 +0.4 212,808 - GFF1029 0.44 -1.3 212,834 - GFF1029 0.47 +0.2 212,838 + GFF1029 0.47 +0.1 212,870 + GFF1029 0.51 +0.2 212,870 + GFF1029 0.51 -1.2 212,870 + GFF1029 0.51 +0.6 212,870 + GFF1029 0.51 -1.2 212,871 - GFF1029 0.51 +0.7 212,871 - GFF1029 0.51 +0.1 212,896 - GFF1029 0.54 +0.6 212,963 + GFF1029 0.61 -1.6 213,086 + GFF1029 0.74 +0.6 213,145 - GFF1029 0.81 -0.2 213,255 + +0.7 213,287 - -1.3 213,432 + -2.4 213,600 - +0.7 213,748 - GFF1030 0.15 +0.0 213,750 + GFF1030 0.16 +0.1 213,896 - GFF1030 0.77 -0.9 213,973 - -0.2 213,978 - -0.5 214,369 + -0.2 214,374 - +1.1 214,479 + -2.2 214,480 - -1.0 214,525 - +1.3 214,772 + GFF1031 0.27 +1.5
Or see this region's nucleotide sequence