Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1000 and GFF1001 are separated by 47 nucleotides GFF1001 and GFF1002 are separated by 157 nucleotides
GFF1000 - Type I restriction-modification system, specificity subunit S (EC 3.1.21.3), at 180,958 to 182,316
GFF1000
GFF1001 - Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72), at 182,364 to 183,953
GFF1001
GFF1002 - Type I restriction-modification system, restriction subunit R (EC 3.1.21.3), at 184,111 to 187,620
GFF1002
Position (kb) 182
183
184 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 181.860 kb on + strand, within GFF1000 at 181.917 kb on - strand, within GFF1000 at 182.234 kb on + strand at 182.774 kb on + strand, within GFF1001 at 182.821 kb on + strand, within GFF1001 at 183.170 kb on + strand, within GFF1001 at 183.170 kb on + strand, within GFF1001 at 183.508 kb on + strand, within GFF1001 at 183.562 kb on + strand, within GFF1001 at 183.562 kb on + strand, within GFF1001 at 183.562 kb on + strand, within GFF1001 at 183.562 kb on + strand, within GFF1001 at 183.563 kb on - strand, within GFF1001 at 183.608 kb on - strand, within GFF1001 at 183.721 kb on - strand, within GFF1001 at 184.054 kb on - strand at 184.054 kb on - strand at 184.104 kb on + strand at 184.113 kb on - strand at 184.133 kb on + strand at 184.133 kb on + strand at 184.648 kb on + strand, within GFF1002 at 184.864 kb on + strand, within GFF1002 at 184.890 kb on - strand, within GFF1002 at 184.890 kb on - strand, within GFF1002 at 184.890 kb on - strand, within GFF1002
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 181,860 + GFF1000 0.66 +0.2 181,917 - GFF1000 0.71 +0.4 182,234 + -1.4 182,774 + GFF1001 0.26 -0.4 182,821 + GFF1001 0.29 +0.4 183,170 + GFF1001 0.51 +0.6 183,170 + GFF1001 0.51 +0.2 183,508 + GFF1001 0.72 +0.7 183,562 + GFF1001 0.75 +0.4 183,562 + GFF1001 0.75 +1.3 183,562 + GFF1001 0.75 -0.3 183,562 + GFF1001 0.75 -1.1 183,563 - GFF1001 0.75 +0.2 183,608 - GFF1001 0.78 +0.8 183,721 - GFF1001 0.85 -0.5 184,054 - +0.8 184,054 - -1.4 184,104 + -2.3 184,113 - +1.4 184,133 + -0.2 184,133 + -0.3 184,648 + GFF1002 0.15 -0.2 184,864 + GFF1002 0.21 +0.3 184,890 - GFF1002 0.22 +0.0 184,890 - GFF1002 0.22 -0.1 184,890 - GFF1002 0.22 -2.9
Or see this region's nucleotide sequence