Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3659 and EX28DRAFT_3660 overlap by 25 nucleotides EX28DRAFT_3660 and EX28DRAFT_3661 are separated by 168 nucleotides
EX28DRAFT_3659: EX28DRAFT_3659 - thiamine ABC transporter, permease protein, at 326,054 to 327,664
_3659
EX28DRAFT_3660: EX28DRAFT_3660 - thiamine ABC transporter, periplasmic binding protein, at 327,640 to 328,623
_3660
EX28DRAFT_3661: EX28DRAFT_3661 - ABC-type uncharacterized transport system, periplasmic component, at 328,792 to 330,447
_3661
Position (kb) 327
328
329 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 326.870 kb on - strand, within EX28DRAFT_3659 at 326.953 kb on - strand, within EX28DRAFT_3659 at 326.974 kb on - strand, within EX28DRAFT_3659 at 327.052 kb on + strand, within EX28DRAFT_3659 at 327.215 kb on - strand, within EX28DRAFT_3659 at 327.341 kb on + strand, within EX28DRAFT_3659 at 327.627 kb on - strand at 327.670 kb on + strand at 327.671 kb on - strand at 327.769 kb on - strand, within EX28DRAFT_3660 at 327.769 kb on - strand, within EX28DRAFT_3660 at 327.779 kb on + strand, within EX28DRAFT_3660 at 327.903 kb on - strand, within EX28DRAFT_3660 at 327.932 kb on + strand, within EX28DRAFT_3660 at 327.932 kb on + strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.933 kb on - strand, within EX28DRAFT_3660 at 327.936 kb on + strand, within EX28DRAFT_3660 at 327.936 kb on + strand, within EX28DRAFT_3660 at 327.937 kb on - strand, within EX28DRAFT_3660 at 327.937 kb on - strand, within EX28DRAFT_3660 at 327.937 kb on - strand, within EX28DRAFT_3660 at 327.978 kb on + strand, within EX28DRAFT_3660 at 327.978 kb on + strand, within EX28DRAFT_3660 at 327.979 kb on - strand, within EX28DRAFT_3660 at 327.981 kb on - strand, within EX28DRAFT_3660 at 327.981 kb on - strand, within EX28DRAFT_3660 at 327.981 kb on - strand, within EX28DRAFT_3660 at 328.238 kb on + strand, within EX28DRAFT_3660 at 328.253 kb on + strand, within EX28DRAFT_3660 at 328.545 kb on - strand at 328.545 kb on - strand at 328.550 kb on + strand at 328.713 kb on + strand at 328.713 kb on + strand at 328.714 kb on - strand at 328.714 kb on - strand at 328.759 kb on + strand at 328.779 kb on + strand at 328.782 kb on + strand at 328.782 kb on + strand at 328.782 kb on + strand at 329.033 kb on + strand, within EX28DRAFT_3661 at 329.033 kb on + strand, within EX28DRAFT_3661 at 329.094 kb on + strand, within EX28DRAFT_3661 at 329.094 kb on + strand, within EX28DRAFT_3661 at 329.129 kb on + strand, within EX28DRAFT_3661 at 329.130 kb on - strand, within EX28DRAFT_3661 at 329.322 kb on - strand, within EX28DRAFT_3661 at 329.322 kb on - strand, within EX28DRAFT_3661 at 329.349 kb on - strand, within EX28DRAFT_3661 at 329.349 kb on - strand, within EX28DRAFT_3661 at 329.349 kb on - strand, within EX28DRAFT_3661 at 329.438 kb on + strand, within EX28DRAFT_3661 at 329.439 kb on - strand, within EX28DRAFT_3661 at 329.441 kb on + strand, within EX28DRAFT_3661 at 329.441 kb on + strand, within EX28DRAFT_3661 at 329.441 kb on + strand, within EX28DRAFT_3661
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 326,870 - EX28DRAFT_3659 0.51 -0.0 326,953 - EX28DRAFT_3659 0.56 +0.7 326,974 - EX28DRAFT_3659 0.57 +0.2 327,052 + EX28DRAFT_3659 0.62 +0.7 327,215 - EX28DRAFT_3659 0.72 +0.6 327,341 + EX28DRAFT_3659 0.80 -0.9 327,627 - -0.2 327,670 + +0.3 327,671 - -0.1 327,769 - EX28DRAFT_3660 0.13 +0.1 327,769 - EX28DRAFT_3660 0.13 +0.0 327,779 + EX28DRAFT_3660 0.14 +0.1 327,903 - EX28DRAFT_3660 0.27 -0.2 327,932 + EX28DRAFT_3660 0.30 +0.3 327,932 + EX28DRAFT_3660 0.30 +1.5 327,933 - EX28DRAFT_3660 0.30 +0.1 327,933 - EX28DRAFT_3660 0.30 +0.6 327,933 - EX28DRAFT_3660 0.30 -0.7 327,933 - EX28DRAFT_3660 0.30 +0.3 327,933 - EX28DRAFT_3660 0.30 +0.1 327,933 - EX28DRAFT_3660 0.30 +0.9 327,933 - EX28DRAFT_3660 0.30 -1.8 327,933 - EX28DRAFT_3660 0.30 +0.8 327,933 - EX28DRAFT_3660 0.30 +0.2 327,936 + EX28DRAFT_3660 0.30 +0.4 327,936 + EX28DRAFT_3660 0.30 +0.2 327,937 - EX28DRAFT_3660 0.30 -0.4 327,937 - EX28DRAFT_3660 0.30 -0.3 327,937 - EX28DRAFT_3660 0.30 +0.2 327,978 + EX28DRAFT_3660 0.34 +0.3 327,978 + EX28DRAFT_3660 0.34 -0.0 327,979 - EX28DRAFT_3660 0.34 +0.3 327,981 - EX28DRAFT_3660 0.35 -0.4 327,981 - EX28DRAFT_3660 0.35 +0.7 327,981 - EX28DRAFT_3660 0.35 +1.3 328,238 + EX28DRAFT_3660 0.61 +0.4 328,253 + EX28DRAFT_3660 0.62 -0.3 328,545 - -0.3 328,545 - -0.4 328,550 + +0.0 328,713 + +0.2 328,713 + +1.5 328,714 - -0.3 328,714 - -0.1 328,759 + -0.4 328,779 + -0.0 328,782 + -0.2 328,782 + -0.3 328,782 + -0.3 329,033 + EX28DRAFT_3661 0.15 +1.0 329,033 + EX28DRAFT_3661 0.15 +0.7 329,094 + EX28DRAFT_3661 0.18 +0.1 329,094 + EX28DRAFT_3661 0.18 -0.2 329,129 + EX28DRAFT_3661 0.20 -0.1 329,130 - EX28DRAFT_3661 0.20 +0.6 329,322 - EX28DRAFT_3661 0.32 +0.3 329,322 - EX28DRAFT_3661 0.32 +0.2 329,349 - EX28DRAFT_3661 0.34 -0.2 329,349 - EX28DRAFT_3661 0.34 +0.4 329,349 - EX28DRAFT_3661 0.34 -0.4 329,438 + EX28DRAFT_3661 0.39 -0.5 329,439 - EX28DRAFT_3661 0.39 -0.1 329,441 + EX28DRAFT_3661 0.39 -4.5 329,441 + EX28DRAFT_3661 0.39 +0.2 329,441 + EX28DRAFT_3661 0.39 +0.1
Or see this region's nucleotide sequence