Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2903 and EX28DRAFT_2904 are separated by 193 nucleotides EX28DRAFT_2904 and EX28DRAFT_2905 are separated by 12 nucleotides
EX28DRAFT_2903: EX28DRAFT_2903 - prolipoprotein diacylglyceryl transferase, at 212,894 to 213,769
_2903
EX28DRAFT_2904: EX28DRAFT_2904 - phosphoenolpyruvate-protein phosphotransferase, at 213,963 to 216,209
_2904
EX28DRAFT_2905: EX28DRAFT_2905 - NTP pyrophosphohydrolases including oxidative damage repair enzymes, at 216,222 to 216,752
_2905
Position (kb)
213
214
215
216
217 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 213.791 kb on - strand at 213.862 kb on - strand at 213.897 kb on + strand at 213.898 kb on - strand at 213.909 kb on - strand at 213.911 kb on - strand at 213.918 kb on + strand at 214.094 kb on + strand at 214.094 kb on + strand at 214.095 kb on - strand at 214.095 kb on - strand at 214.108 kb on + strand at 214.108 kb on + strand at 214.109 kb on - strand at 214.109 kb on - strand at 214.215 kb on + strand, within EX28DRAFT_2904 at 214.228 kb on + strand, within EX28DRAFT_2904 at 214.291 kb on + strand, within EX28DRAFT_2904 at 214.291 kb on + strand, within EX28DRAFT_2904 at 214.334 kb on + strand, within EX28DRAFT_2904 at 214.334 kb on + strand, within EX28DRAFT_2904 at 214.334 kb on + strand, within EX28DRAFT_2904 at 214.335 kb on - strand, within EX28DRAFT_2904 at 214.335 kb on - strand, within EX28DRAFT_2904 at 214.336 kb on + strand, within EX28DRAFT_2904 at 214.336 kb on + strand, within EX28DRAFT_2904 at 214.336 kb on + strand, within EX28DRAFT_2904 at 214.337 kb on - strand, within EX28DRAFT_2904 at 214.337 kb on - strand, within EX28DRAFT_2904 at 214.367 kb on + strand, within EX28DRAFT_2904 at 214.400 kb on + strand, within EX28DRAFT_2904 at 214.545 kb on + strand, within EX28DRAFT_2904 at 214.546 kb on - strand, within EX28DRAFT_2904 at 214.679 kb on + strand, within EX28DRAFT_2904 at 214.681 kb on + strand, within EX28DRAFT_2904 at 214.681 kb on + strand, within EX28DRAFT_2904 at 214.681 kb on + strand, within EX28DRAFT_2904 at 214.682 kb on - strand, within EX28DRAFT_2904 at 214.682 kb on - strand, within EX28DRAFT_2904 at 214.682 kb on - strand, within EX28DRAFT_2904 at 214.682 kb on - strand, within EX28DRAFT_2904 at 214.763 kb on - strand, within EX28DRAFT_2904 at 214.828 kb on + strand, within EX28DRAFT_2904 at 214.922 kb on + strand, within EX28DRAFT_2904 at 214.922 kb on + strand, within EX28DRAFT_2904 at 214.965 kb on + strand, within EX28DRAFT_2904 at 214.966 kb on - strand, within EX28DRAFT_2904 at 214.996 kb on + strand, within EX28DRAFT_2904 at 215.056 kb on - strand, within EX28DRAFT_2904 at 215.132 kb on + strand, within EX28DRAFT_2904 at 215.276 kb on + strand, within EX28DRAFT_2904 at 215.533 kb on + strand, within EX28DRAFT_2904 at 215.814 kb on + strand, within EX28DRAFT_2904 at 215.815 kb on - strand, within EX28DRAFT_2904 at 215.815 kb on - strand, within EX28DRAFT_2904 at 215.815 kb on - strand, within EX28DRAFT_2904 at 215.917 kb on + strand, within EX28DRAFT_2904 at 215.917 kb on + strand, within EX28DRAFT_2904 at 215.918 kb on - strand, within EX28DRAFT_2904 at 215.921 kb on + strand, within EX28DRAFT_2904 at 215.990 kb on + strand at 215.991 kb on - strand at 216.058 kb on + strand at 216.059 kb on - strand at 216.061 kb on + strand at 216.061 kb on + strand at 216.062 kb on - strand at 216.088 kb on + strand at 216.141 kb on - strand at 216.187 kb on - strand at 216.218 kb on + strand at 216.219 kb on - strand at 216.224 kb on - strand at 216.234 kb on - strand at 216.776 kb on - strand at 216.777 kb on + strand at 216.778 kb on - strand at 216.779 kb on + strand at 216.780 kb on - strand at 216.780 kb on - strand at 216.780 kb on - strand at 216.780 kb on - strand at 216.825 kb on + strand at 216.825 kb on + strand at 216.840 kb on + strand at 216.866 kb on - strand at 216.930 kb on + strand at 216.930 kb on + strand at 216.931 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 213,791 - -0.0 213,862 - -0.4 213,897 + -0.1 213,898 - +1.1 213,909 - +0.3 213,911 - +0.4 213,918 + -0.3 214,094 + +0.3 214,094 + +0.4 214,095 - -0.1 214,095 - +0.6 214,108 + +0.4 214,108 + +0.2 214,109 - -0.2 214,109 - +0.0 214,215 + EX28DRAFT_2904 0.11 -0.6 214,228 + EX28DRAFT_2904 0.12 -0.1 214,291 + EX28DRAFT_2904 0.15 -0.1 214,291 + EX28DRAFT_2904 0.15 +0.2 214,334 + EX28DRAFT_2904 0.17 +0.3 214,334 + EX28DRAFT_2904 0.17 +1.1 214,334 + EX28DRAFT_2904 0.17 +0.7 214,335 - EX28DRAFT_2904 0.17 +0.2 214,335 - EX28DRAFT_2904 0.17 +0.5 214,336 + EX28DRAFT_2904 0.17 +0.1 214,336 + EX28DRAFT_2904 0.17 -0.6 214,336 + EX28DRAFT_2904 0.17 +0.8 214,337 - EX28DRAFT_2904 0.17 +0.5 214,337 - EX28DRAFT_2904 0.17 +0.7 214,367 + EX28DRAFT_2904 0.18 +1.4 214,400 + EX28DRAFT_2904 0.19 +0.7 214,545 + EX28DRAFT_2904 0.26 +1.0 214,546 - EX28DRAFT_2904 0.26 +0.8 214,679 + EX28DRAFT_2904 0.32 +0.2 214,681 + EX28DRAFT_2904 0.32 -0.5 214,681 + EX28DRAFT_2904 0.32 +0.7 214,681 + EX28DRAFT_2904 0.32 -0.3 214,682 - EX28DRAFT_2904 0.32 +0.4 214,682 - EX28DRAFT_2904 0.32 +0.2 214,682 - EX28DRAFT_2904 0.32 -0.1 214,682 - EX28DRAFT_2904 0.32 -0.2 214,763 - EX28DRAFT_2904 0.36 -0.6 214,828 + EX28DRAFT_2904 0.38 +0.2 214,922 + EX28DRAFT_2904 0.43 +0.6 214,922 + EX28DRAFT_2904 0.43 +0.1 214,965 + EX28DRAFT_2904 0.45 -0.3 214,966 - EX28DRAFT_2904 0.45 +0.1 214,996 + EX28DRAFT_2904 0.46 +0.3 215,056 - EX28DRAFT_2904 0.49 +0.5 215,132 + EX28DRAFT_2904 0.52 +0.3 215,276 + EX28DRAFT_2904 0.58 -1.3 215,533 + EX28DRAFT_2904 0.70 +0.6 215,814 + EX28DRAFT_2904 0.82 -0.2 215,815 - EX28DRAFT_2904 0.82 -1.3 215,815 - EX28DRAFT_2904 0.82 +0.6 215,815 - EX28DRAFT_2904 0.82 -0.5 215,917 + EX28DRAFT_2904 0.87 +0.3 215,917 + EX28DRAFT_2904 0.87 +0.3 215,918 - EX28DRAFT_2904 0.87 +0.4 215,921 + EX28DRAFT_2904 0.87 -0.1 215,990 + +0.0 215,991 - +0.5 216,058 + +1.5 216,059 - -3.9 216,061 + -0.6 216,061 + -0.0 216,062 - +0.2 216,088 + +0.6 216,141 - +0.7 216,187 - +0.1 216,218 + +0.0 216,219 - -0.1 216,224 - +0.4 216,234 - -0.2 216,776 - -0.2 216,777 + -1.9 216,778 - +0.4 216,779 + -0.9 216,780 - -0.3 216,780 - +0.3 216,780 - -0.1 216,780 - -0.6 216,825 + -0.1 216,825 + +0.1 216,840 + +0.2 216,866 - -3.6 216,930 + +1.0 216,930 + +0.1 216,931 - -0.3
Or see this region's nucleotide sequence