Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1751 and EX28DRAFT_1752 overlap by 4 nucleotides EX28DRAFT_1752 and EX28DRAFT_1753 are separated by 433 nucleotides
EX28DRAFT_1751: EX28DRAFT_1751 - arginine N-succinyltransferase, at 1,766,043 to 1,767,077
_1751
EX28DRAFT_1752: EX28DRAFT_1752 - transaminase, acetylornithine/succinylornithine family/succinylornithine transaminase family, at 1,767,074 to 1,768,294
_1752
EX28DRAFT_1753: EX28DRAFT_1753 - exodeoxyribonuclease III, at 1,768,728 to 1,769,534
_1753
Position (kb)
1767
1768
1769 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1766.100 kb on - strand at 1766.189 kb on - strand, within EX28DRAFT_1751 at 1766.332 kb on + strand, within EX28DRAFT_1751 at 1766.333 kb on - strand, within EX28DRAFT_1751 at 1766.440 kb on + strand, within EX28DRAFT_1751 at 1766.441 kb on - strand, within EX28DRAFT_1751 at 1766.441 kb on - strand, within EX28DRAFT_1751 at 1766.441 kb on - strand, within EX28DRAFT_1751 at 1766.441 kb on - strand, within EX28DRAFT_1751 at 1766.444 kb on + strand, within EX28DRAFT_1751 at 1766.444 kb on + strand, within EX28DRAFT_1751 at 1766.444 kb on + strand, within EX28DRAFT_1751 at 1766.445 kb on - strand, within EX28DRAFT_1751 at 1766.500 kb on + strand, within EX28DRAFT_1751 at 1766.564 kb on - strand, within EX28DRAFT_1751 at 1766.637 kb on - strand, within EX28DRAFT_1751 at 1766.642 kb on + strand, within EX28DRAFT_1751 at 1766.642 kb on + strand, within EX28DRAFT_1751 at 1766.666 kb on - strand, within EX28DRAFT_1751 at 1766.767 kb on + strand, within EX28DRAFT_1751 at 1766.767 kb on + strand, within EX28DRAFT_1751 at 1767.180 kb on - strand at 1767.180 kb on - strand at 1767.235 kb on + strand, within EX28DRAFT_1752 at 1767.242 kb on - strand, within EX28DRAFT_1752 at 1767.580 kb on + strand, within EX28DRAFT_1752 at 1767.580 kb on + strand, within EX28DRAFT_1752 at 1767.582 kb on + strand, within EX28DRAFT_1752 at 1767.582 kb on + strand, within EX28DRAFT_1752 at 1767.582 kb on + strand, within EX28DRAFT_1752 at 1767.583 kb on - strand, within EX28DRAFT_1752 at 1767.583 kb on - strand, within EX28DRAFT_1752 at 1767.583 kb on - strand, within EX28DRAFT_1752 at 1767.588 kb on + strand, within EX28DRAFT_1752 at 1767.589 kb on - strand, within EX28DRAFT_1752 at 1767.786 kb on + strand, within EX28DRAFT_1752 at 1767.835 kb on - strand, within EX28DRAFT_1752 at 1767.989 kb on - strand, within EX28DRAFT_1752 at 1768.173 kb on + strand at 1768.174 kb on - strand at 1768.174 kb on - strand at 1768.243 kb on - strand at 1768.280 kb on - strand at 1768.294 kb on + strand at 1768.295 kb on - strand at 1768.362 kb on + strand at 1768.402 kb on - strand at 1768.536 kb on + strand at 1768.621 kb on + strand at 1768.639 kb on - strand at 1768.651 kb on + strand at 1768.745 kb on + strand at 1768.847 kb on + strand, within EX28DRAFT_1753 at 1768.847 kb on + strand, within EX28DRAFT_1753 at 1768.847 kb on + strand, within EX28DRAFT_1753 at 1768.847 kb on + strand, within EX28DRAFT_1753 at 1768.848 kb on - strand, within EX28DRAFT_1753 at 1768.848 kb on - strand, within EX28DRAFT_1753 at 1768.848 kb on - strand, within EX28DRAFT_1753 at 1768.848 kb on - strand, within EX28DRAFT_1753 at 1768.893 kb on + strand, within EX28DRAFT_1753 at 1768.893 kb on + strand, within EX28DRAFT_1753 at 1768.893 kb on + strand, within EX28DRAFT_1753 at 1768.894 kb on - strand, within EX28DRAFT_1753 at 1768.894 kb on - strand, within EX28DRAFT_1753 at 1769.053 kb on - strand, within EX28DRAFT_1753 at 1769.131 kb on - strand, within EX28DRAFT_1753 at 1769.186 kb on + strand, within EX28DRAFT_1753
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,766,100 - -0.4 1,766,189 - EX28DRAFT_1751 0.14 +0.1 1,766,332 + EX28DRAFT_1751 0.28 -0.1 1,766,333 - EX28DRAFT_1751 0.28 -0.2 1,766,440 + EX28DRAFT_1751 0.38 -0.3 1,766,441 - EX28DRAFT_1751 0.38 -0.9 1,766,441 - EX28DRAFT_1751 0.38 +0.7 1,766,441 - EX28DRAFT_1751 0.38 -0.2 1,766,441 - EX28DRAFT_1751 0.38 -1.5 1,766,444 + EX28DRAFT_1751 0.39 +0.1 1,766,444 + EX28DRAFT_1751 0.39 -0.2 1,766,444 + EX28DRAFT_1751 0.39 +1.9 1,766,445 - EX28DRAFT_1751 0.39 +1.5 1,766,500 + EX28DRAFT_1751 0.44 +0.5 1,766,564 - EX28DRAFT_1751 0.50 -0.4 1,766,637 - EX28DRAFT_1751 0.57 -0.3 1,766,642 + EX28DRAFT_1751 0.58 +0.1 1,766,642 + EX28DRAFT_1751 0.58 +0.4 1,766,666 - EX28DRAFT_1751 0.60 +0.2 1,766,767 + EX28DRAFT_1751 0.70 -0.3 1,766,767 + EX28DRAFT_1751 0.70 -0.5 1,767,180 - -0.4 1,767,180 - +0.8 1,767,235 + EX28DRAFT_1752 0.13 +1.7 1,767,242 - EX28DRAFT_1752 0.14 -0.0 1,767,580 + EX28DRAFT_1752 0.41 -0.1 1,767,580 + EX28DRAFT_1752 0.41 +0.4 1,767,582 + EX28DRAFT_1752 0.42 -0.8 1,767,582 + EX28DRAFT_1752 0.42 +0.5 1,767,582 + EX28DRAFT_1752 0.42 +0.4 1,767,583 - EX28DRAFT_1752 0.42 +0.2 1,767,583 - EX28DRAFT_1752 0.42 +1.7 1,767,583 - EX28DRAFT_1752 0.42 +0.4 1,767,588 + EX28DRAFT_1752 0.42 +0.8 1,767,589 - EX28DRAFT_1752 0.42 -0.0 1,767,786 + EX28DRAFT_1752 0.58 -0.3 1,767,835 - EX28DRAFT_1752 0.62 +0.0 1,767,989 - EX28DRAFT_1752 0.75 +1.2 1,768,173 + -0.5 1,768,174 - +0.7 1,768,174 - -0.3 1,768,243 - -1.1 1,768,280 - -0.5 1,768,294 + -0.2 1,768,295 - +0.9 1,768,362 + -0.8 1,768,402 - -0.1 1,768,536 + +0.1 1,768,621 + -1.6 1,768,639 - +1.3 1,768,651 + -0.9 1,768,745 + -0.2 1,768,847 + EX28DRAFT_1753 0.15 -0.4 1,768,847 + EX28DRAFT_1753 0.15 +0.1 1,768,847 + EX28DRAFT_1753 0.15 +0.5 1,768,847 + EX28DRAFT_1753 0.15 +1.0 1,768,848 - EX28DRAFT_1753 0.15 -0.0 1,768,848 - EX28DRAFT_1753 0.15 -0.0 1,768,848 - EX28DRAFT_1753 0.15 -3.3 1,768,848 - EX28DRAFT_1753 0.15 +1.5 1,768,893 + EX28DRAFT_1753 0.20 -0.8 1,768,893 + EX28DRAFT_1753 0.20 -0.6 1,768,893 + EX28DRAFT_1753 0.20 +0.1 1,768,894 - EX28DRAFT_1753 0.21 -0.5 1,768,894 - EX28DRAFT_1753 0.21 +0.2 1,769,053 - EX28DRAFT_1753 0.40 +0.1 1,769,131 - EX28DRAFT_1753 0.50 +0.5 1,769,186 + EX28DRAFT_1753 0.57 +0.4
Or see this region's nucleotide sequence