Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1646 and EX28DRAFT_1647 are separated by 41 nucleotides EX28DRAFT_1647 and EX28DRAFT_1648 are separated by 53 nucleotides EX28DRAFT_1648 and EX28DRAFT_1649 are separated by 100 nucleotides EX28DRAFT_1649 and EX28DRAFT_1650 are separated by 95 nucleotides
EX28DRAFT_1646: EX28DRAFT_1646 - Predicted membrane-associated, metal-dependent hydrolase, at 1,662,489 to 1,664,114
_1646
EX28DRAFT_1647: EX28DRAFT_1647 - transcriptional regulator, TetR family, at 1,664,156 to 1,664,755
_1647
EX28DRAFT_1648: EX28DRAFT_1648 - NADH:flavin oxidoreductases, Old Yellow Enzyme family, at 1,664,809 to 1,665,906
_1648
EX28DRAFT_1649: EX28DRAFT_1649 - lactoylglutathione lyase, at 1,666,007 to 1,666,414
_1649
EX28DRAFT_1650: EX28DRAFT_1650 - ribonuclease T, at 1,666,510 to 1,667,160
_1650
Position (kb)
1664
1665
1666 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1663.871 kb on + strand, within EX28DRAFT_1646 at 1663.875 kb on + strand, within EX28DRAFT_1646 at 1663.923 kb on - strand, within EX28DRAFT_1646 at 1663.932 kb on - strand, within EX28DRAFT_1646 at 1664.030 kb on - strand at 1664.091 kb on - strand at 1664.092 kb on + strand at 1664.092 kb on + strand at 1664.093 kb on - strand at 1664.093 kb on - strand at 1664.093 kb on - strand at 1664.094 kb on - strand at 1664.167 kb on + strand at 1664.297 kb on + strand, within EX28DRAFT_1647 at 1664.395 kb on + strand, within EX28DRAFT_1647 at 1664.395 kb on + strand, within EX28DRAFT_1647 at 1664.420 kb on + strand, within EX28DRAFT_1647 at 1664.519 kb on - strand, within EX28DRAFT_1647 at 1664.536 kb on + strand, within EX28DRAFT_1647 at 1664.643 kb on + strand, within EX28DRAFT_1647 at 1664.644 kb on - strand, within EX28DRAFT_1647 at 1664.645 kb on + strand, within EX28DRAFT_1647 at 1664.646 kb on - strand, within EX28DRAFT_1647 at 1664.665 kb on + strand, within EX28DRAFT_1647 at 1664.738 kb on - strand at 1664.738 kb on - strand at 1664.754 kb on - strand at 1664.754 kb on - strand at 1664.766 kb on + strand at 1664.770 kb on + strand at 1664.808 kb on + strand at 1664.809 kb on - strand at 1664.830 kb on - strand at 1664.871 kb on - strand at 1664.871 kb on - strand at 1664.874 kb on + strand at 1664.941 kb on + strand, within EX28DRAFT_1648 at 1664.941 kb on + strand, within EX28DRAFT_1648 at 1664.941 kb on + strand, within EX28DRAFT_1648 at 1664.941 kb on + strand, within EX28DRAFT_1648 at 1664.942 kb on - strand, within EX28DRAFT_1648 at 1665.195 kb on + strand, within EX28DRAFT_1648 at 1665.198 kb on - strand, within EX28DRAFT_1648 at 1665.399 kb on + strand, within EX28DRAFT_1648 at 1665.474 kb on - strand, within EX28DRAFT_1648 at 1665.574 kb on + strand, within EX28DRAFT_1648 at 1665.718 kb on + strand, within EX28DRAFT_1648 at 1665.719 kb on - strand, within EX28DRAFT_1648 at 1665.721 kb on - strand, within EX28DRAFT_1648 at 1665.759 kb on - strand, within EX28DRAFT_1648 at 1665.883 kb on + strand at 1665.893 kb on - strand at 1665.982 kb on + strand at 1665.983 kb on - strand at 1665.983 kb on - strand at 1666.006 kb on + strand at 1666.007 kb on - strand at 1666.069 kb on + strand, within EX28DRAFT_1649 at 1666.121 kb on + strand, within EX28DRAFT_1649 at 1666.122 kb on - strand, within EX28DRAFT_1649 at 1666.122 kb on - strand, within EX28DRAFT_1649 at 1666.181 kb on + strand, within EX28DRAFT_1649 at 1666.182 kb on - strand, within EX28DRAFT_1649 at 1666.182 kb on - strand, within EX28DRAFT_1649 at 1666.183 kb on + strand, within EX28DRAFT_1649 at 1666.184 kb on - strand, within EX28DRAFT_1649 at 1666.202 kb on + strand, within EX28DRAFT_1649 at 1666.202 kb on + strand, within EX28DRAFT_1649 at 1666.203 kb on - strand, within EX28DRAFT_1649 at 1666.315 kb on - strand, within EX28DRAFT_1649 at 1666.440 kb on + strand at 1666.498 kb on + strand at 1666.786 kb on + strand, within EX28DRAFT_1650
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,663,871 + EX28DRAFT_1646 0.85 +0.1 1,663,875 + EX28DRAFT_1646 0.85 +0.3 1,663,923 - EX28DRAFT_1646 0.88 +0.6 1,663,932 - EX28DRAFT_1646 0.89 -0.7 1,664,030 - -0.6 1,664,091 - -0.1 1,664,092 + +0.1 1,664,092 + +0.3 1,664,093 - +0.1 1,664,093 - -0.6 1,664,093 - -0.5 1,664,094 - +0.2 1,664,167 + +0.4 1,664,297 + EX28DRAFT_1647 0.23 +0.2 1,664,395 + EX28DRAFT_1647 0.40 -0.3 1,664,395 + EX28DRAFT_1647 0.40 +0.8 1,664,420 + EX28DRAFT_1647 0.44 -3.7 1,664,519 - EX28DRAFT_1647 0.60 +1.1 1,664,536 + EX28DRAFT_1647 0.63 +0.4 1,664,643 + EX28DRAFT_1647 0.81 -0.9 1,664,644 - EX28DRAFT_1647 0.81 +0.4 1,664,645 + EX28DRAFT_1647 0.81 +0.4 1,664,646 - EX28DRAFT_1647 0.82 -0.7 1,664,665 + EX28DRAFT_1647 0.85 -0.2 1,664,738 - -2.1 1,664,738 - -0.2 1,664,754 - +0.6 1,664,754 - +0.4 1,664,766 + +0.2 1,664,770 + -0.2 1,664,808 + +0.1 1,664,809 - -0.1 1,664,830 - -0.9 1,664,871 - +0.4 1,664,871 - +1.2 1,664,874 + +0.4 1,664,941 + EX28DRAFT_1648 0.12 -0.2 1,664,941 + EX28DRAFT_1648 0.12 -0.5 1,664,941 + EX28DRAFT_1648 0.12 -0.3 1,664,941 + EX28DRAFT_1648 0.12 -0.2 1,664,942 - EX28DRAFT_1648 0.12 -0.4 1,665,195 + EX28DRAFT_1648 0.35 -0.2 1,665,198 - EX28DRAFT_1648 0.35 +0.1 1,665,399 + EX28DRAFT_1648 0.54 +0.1 1,665,474 - EX28DRAFT_1648 0.61 -0.9 1,665,574 + EX28DRAFT_1648 0.70 +0.4 1,665,718 + EX28DRAFT_1648 0.83 +0.3 1,665,719 - EX28DRAFT_1648 0.83 +0.0 1,665,721 - EX28DRAFT_1648 0.83 -1.1 1,665,759 - EX28DRAFT_1648 0.87 -0.6 1,665,883 + +0.2 1,665,893 - -0.0 1,665,982 + -0.0 1,665,983 - +0.3 1,665,983 - +0.9 1,666,006 + -0.3 1,666,007 - +0.5 1,666,069 + EX28DRAFT_1649 0.15 -0.3 1,666,121 + EX28DRAFT_1649 0.28 +2.5 1,666,122 - EX28DRAFT_1649 0.28 -0.5 1,666,122 - EX28DRAFT_1649 0.28 +0.1 1,666,181 + EX28DRAFT_1649 0.43 -1.4 1,666,182 - EX28DRAFT_1649 0.43 +0.6 1,666,182 - EX28DRAFT_1649 0.43 -1.2 1,666,183 + EX28DRAFT_1649 0.43 +0.7 1,666,184 - EX28DRAFT_1649 0.43 +1.4 1,666,202 + EX28DRAFT_1649 0.48 -0.1 1,666,202 + EX28DRAFT_1649 0.48 +0.2 1,666,203 - EX28DRAFT_1649 0.48 -0.2 1,666,315 - EX28DRAFT_1649 0.75 +1.0 1,666,440 + +0.4 1,666,498 + -1.1 1,666,786 + EX28DRAFT_1650 0.42 -0.4
Or see this region's nucleotide sequence