Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0530 and EX28DRAFT_0531 are separated by 52 nucleotides EX28DRAFT_0531 and EX28DRAFT_0532 are separated by 192 nucleotides
EX28DRAFT_0530: EX28DRAFT_0530 - nucleotide sugar dehydrogenase, at 524,652 to 525,818
_0530
EX28DRAFT_0531: EX28DRAFT_0531 - Nucleoside-diphosphate-sugar epimerases, at 525,871 to 526,875
_0531
EX28DRAFT_0532: EX28DRAFT_0532 - Chain length determinant protein, at 527,068 to 528,048
_0532
Position (kb) 525
526
527 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 525.960 kb on - strand at 526.103 kb on + strand, within EX28DRAFT_0531 at 526.176 kb on + strand, within EX28DRAFT_0531 at 526.177 kb on - strand, within EX28DRAFT_0531 at 526.195 kb on - strand, within EX28DRAFT_0531 at 526.220 kb on + strand, within EX28DRAFT_0531 at 526.221 kb on - strand, within EX28DRAFT_0531 at 526.248 kb on - strand, within EX28DRAFT_0531 at 526.251 kb on + strand, within EX28DRAFT_0531 at 526.253 kb on + strand, within EX28DRAFT_0531 at 526.290 kb on + strand, within EX28DRAFT_0531 at 526.291 kb on - strand, within EX28DRAFT_0531 at 526.296 kb on + strand, within EX28DRAFT_0531 at 526.331 kb on + strand, within EX28DRAFT_0531 at 526.371 kb on - strand, within EX28DRAFT_0531 at 526.371 kb on - strand, within EX28DRAFT_0531 at 526.383 kb on - strand, within EX28DRAFT_0531 at 526.489 kb on + strand, within EX28DRAFT_0531 at 526.793 kb on - strand at 526.929 kb on + strand at 526.930 kb on - strand at 526.935 kb on - strand at 526.975 kb on + strand at 526.976 kb on - strand at 526.976 kb on - strand at 527.013 kb on + strand at 527.013 kb on + strand at 527.014 kb on - strand at 527.014 kb on - strand at 527.014 kb on - strand at 527.014 kb on - strand at 527.014 kb on - strand at 527.015 kb on + strand at 527.015 kb on + strand at 527.015 kb on + strand at 527.015 kb on + strand at 527.015 kb on + strand at 527.015 kb on + strand at 527.015 kb on + strand at 527.016 kb on - strand at 527.016 kb on - strand at 527.016 kb on - strand at 527.020 kb on + strand at 527.020 kb on + strand at 527.021 kb on - strand at 527.030 kb on + strand at 527.033 kb on + strand at 527.034 kb on - strand at 527.034 kb on - strand at 527.068 kb on - strand at 527.127 kb on - strand at 527.135 kb on + strand at 527.190 kb on + strand, within EX28DRAFT_0532 at 527.213 kb on + strand, within EX28DRAFT_0532 at 527.284 kb on + strand, within EX28DRAFT_0532 at 527.285 kb on - strand, within EX28DRAFT_0532 at 527.285 kb on - strand, within EX28DRAFT_0532 at 527.365 kb on - strand, within EX28DRAFT_0532 at 527.412 kb on - strand, within EX28DRAFT_0532 at 527.424 kb on + strand, within EX28DRAFT_0532 at 527.476 kb on - strand, within EX28DRAFT_0532 at 527.518 kb on + strand, within EX28DRAFT_0532 at 527.518 kb on + strand, within EX28DRAFT_0532 at 527.615 kb on + strand, within EX28DRAFT_0532 at 527.735 kb on + strand, within EX28DRAFT_0532 at 527.735 kb on + strand, within EX28DRAFT_0532 at 527.830 kb on + strand, within EX28DRAFT_0532 at 527.874 kb on - strand, within EX28DRAFT_0532 at 527.874 kb on - strand, within EX28DRAFT_0532
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 525,960 - -1.5 526,103 + EX28DRAFT_0531 0.23 -2.3 526,176 + EX28DRAFT_0531 0.30 -1.3 526,177 - EX28DRAFT_0531 0.30 -1.1 526,195 - EX28DRAFT_0531 0.32 -0.6 526,220 + EX28DRAFT_0531 0.35 -1.3 526,221 - EX28DRAFT_0531 0.35 -0.6 526,248 - EX28DRAFT_0531 0.38 -2.3 526,251 + EX28DRAFT_0531 0.38 -1.2 526,253 + EX28DRAFT_0531 0.38 +1.3 526,290 + EX28DRAFT_0531 0.42 -1.7 526,291 - EX28DRAFT_0531 0.42 -1.2 526,296 + EX28DRAFT_0531 0.42 -1.7 526,331 + EX28DRAFT_0531 0.46 -0.2 526,371 - EX28DRAFT_0531 0.50 -2.3 526,371 - EX28DRAFT_0531 0.50 +0.5 526,383 - EX28DRAFT_0531 0.51 +1.2 526,489 + EX28DRAFT_0531 0.61 -2.3 526,793 - -2.0 526,929 + -0.9 526,930 - -0.1 526,935 - +0.1 526,975 + +0.4 526,976 - -0.0 526,976 - -0.5 527,013 + -0.9 527,013 + -0.7 527,014 - +0.1 527,014 - +0.1 527,014 - +0.4 527,014 - +0.4 527,014 - -0.5 527,015 + +0.6 527,015 + +0.1 527,015 + -0.8 527,015 + -0.6 527,015 + -0.4 527,015 + +0.2 527,015 + -0.3 527,016 - -0.3 527,016 - -0.9 527,016 - -0.1 527,020 + -0.4 527,020 + +0.9 527,021 - +0.9 527,030 + +0.4 527,033 + +2.0 527,034 - -0.5 527,034 - +0.2 527,068 - +0.7 527,127 - +1.0 527,135 + +0.2 527,190 + EX28DRAFT_0532 0.12 -1.3 527,213 + EX28DRAFT_0532 0.15 +0.2 527,284 + EX28DRAFT_0532 0.22 -0.6 527,285 - EX28DRAFT_0532 0.22 +0.1 527,285 - EX28DRAFT_0532 0.22 -2.3 527,365 - EX28DRAFT_0532 0.30 -0.4 527,412 - EX28DRAFT_0532 0.35 -0.3 527,424 + EX28DRAFT_0532 0.36 +0.3 527,476 - EX28DRAFT_0532 0.42 +0.0 527,518 + EX28DRAFT_0532 0.46 +0.0 527,518 + EX28DRAFT_0532 0.46 +0.2 527,615 + EX28DRAFT_0532 0.56 -0.2 527,735 + EX28DRAFT_0532 0.68 -2.9 527,735 + EX28DRAFT_0532 0.68 -0.4 527,830 + EX28DRAFT_0532 0.78 +0.6 527,874 - EX28DRAFT_0532 0.82 -0.2 527,874 - EX28DRAFT_0532 0.82 -0.1
Or see this region's nucleotide sequence