Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0503 and EX28DRAFT_0504 are separated by 2 nucleotides EX28DRAFT_0504 and EX28DRAFT_0505 overlap by 4 nucleotides EX28DRAFT_0505 and EX28DRAFT_0506 are separated by 3 nucleotides
EX28DRAFT_0503: EX28DRAFT_0503 - Nucleoside-diphosphate-sugar epimerases, at 494,590 to 495,555
_0503
EX28DRAFT_0504: EX28DRAFT_0504 - ADP-ribose pyrophosphatase, at 495,558 to 496,037
_0504
EX28DRAFT_0505: EX28DRAFT_0505 - colanic acid biosynthesis glycosyl transferase WcaI, at 496,034 to 497,257
_0505
EX28DRAFT_0506: EX28DRAFT_0506 - mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase, at 497,261 to 498,697
_0506
Position (kb) 496
497
498 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 495.049 kb on + strand, within EX28DRAFT_0503 at 495.378 kb on - strand, within EX28DRAFT_0503 at 495.511 kb on - strand at 495.514 kb on + strand at 495.514 kb on + strand at 495.515 kb on - strand at 495.589 kb on + strand at 495.822 kb on + strand, within EX28DRAFT_0504 at 495.823 kb on - strand, within EX28DRAFT_0504 at 495.830 kb on + strand, within EX28DRAFT_0504 at 495.861 kb on + strand, within EX28DRAFT_0504 at 495.862 kb on - strand, within EX28DRAFT_0504 at 495.997 kb on - strand at 496.033 kb on + strand at 496.034 kb on - strand at 496.048 kb on - strand at 496.061 kb on + strand at 496.062 kb on - strand at 496.250 kb on + strand, within EX28DRAFT_0505 at 496.441 kb on + strand, within EX28DRAFT_0505 at 496.441 kb on + strand, within EX28DRAFT_0505 at 496.452 kb on - strand, within EX28DRAFT_0505 at 496.560 kb on - strand, within EX28DRAFT_0505 at 496.917 kb on - strand, within EX28DRAFT_0505 at 497.067 kb on + strand, within EX28DRAFT_0505 at 497.192 kb on + strand at 497.192 kb on + strand at 497.236 kb on + strand at 497.237 kb on - strand at 497.237 kb on - strand at 497.245 kb on + strand at 497.246 kb on - strand at 497.247 kb on + strand at 497.247 kb on + strand at 497.247 kb on + strand at 497.247 kb on + strand at 497.248 kb on - strand at 497.458 kb on + strand, within EX28DRAFT_0506 at 497.458 kb on + strand, within EX28DRAFT_0506 at 497.738 kb on + strand, within EX28DRAFT_0506 at 497.837 kb on + strand, within EX28DRAFT_0506 at 497.837 kb on + strand, within EX28DRAFT_0506 at 497.838 kb on - strand, within EX28DRAFT_0506 at 497.855 kb on + strand, within EX28DRAFT_0506 at 497.855 kb on + strand, within EX28DRAFT_0506 at 497.855 kb on + strand, within EX28DRAFT_0506 at 497.856 kb on - strand, within EX28DRAFT_0506 at 497.856 kb on - strand, within EX28DRAFT_0506 at 497.856 kb on - strand, within EX28DRAFT_0506 at 497.859 kb on - strand, within EX28DRAFT_0506 at 497.859 kb on - strand, within EX28DRAFT_0506 at 497.859 kb on - strand, within EX28DRAFT_0506 at 498.053 kb on - strand, within EX28DRAFT_0506 at 498.195 kb on - strand, within EX28DRAFT_0506
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 495,049 + EX28DRAFT_0503 0.48 +0.0 495,378 - EX28DRAFT_0503 0.82 -0.1 495,511 - +0.4 495,514 + -0.4 495,514 + -0.2 495,515 - -0.7 495,589 + -0.3 495,822 + EX28DRAFT_0504 0.55 +0.2 495,823 - EX28DRAFT_0504 0.55 -1.7 495,830 + EX28DRAFT_0504 0.57 +0.4 495,861 + EX28DRAFT_0504 0.63 +0.6 495,862 - EX28DRAFT_0504 0.63 +0.5 495,997 - -0.0 496,033 + -0.0 496,034 - +0.0 496,048 - -0.3 496,061 + -1.4 496,062 - -0.5 496,250 + EX28DRAFT_0505 0.18 -0.0 496,441 + EX28DRAFT_0505 0.33 +0.9 496,441 + EX28DRAFT_0505 0.33 -0.4 496,452 - EX28DRAFT_0505 0.34 +0.3 496,560 - EX28DRAFT_0505 0.43 +0.2 496,917 - EX28DRAFT_0505 0.72 +1.8 497,067 + EX28DRAFT_0505 0.84 +0.7 497,192 + -0.0 497,192 + +0.9 497,236 + -3.6 497,237 - +0.1 497,237 - -0.7 497,245 + -0.7 497,246 - -0.9 497,247 + +0.7 497,247 + -1.2 497,247 + +0.0 497,247 + +0.2 497,248 - +0.2 497,458 + EX28DRAFT_0506 0.14 +0.5 497,458 + EX28DRAFT_0506 0.14 +0.3 497,738 + EX28DRAFT_0506 0.33 +0.5 497,837 + EX28DRAFT_0506 0.40 +0.3 497,837 + EX28DRAFT_0506 0.40 -0.2 497,838 - EX28DRAFT_0506 0.40 -0.0 497,855 + EX28DRAFT_0506 0.41 +0.4 497,855 + EX28DRAFT_0506 0.41 -0.2 497,855 + EX28DRAFT_0506 0.41 -0.6 497,856 - EX28DRAFT_0506 0.41 +0.2 497,856 - EX28DRAFT_0506 0.41 +0.2 497,856 - EX28DRAFT_0506 0.41 +0.2 497,859 - EX28DRAFT_0506 0.42 -0.0 497,859 - EX28DRAFT_0506 0.42 +0.1 497,859 - EX28DRAFT_0506 0.42 -0.8 498,053 - EX28DRAFT_0506 0.55 +0.1 498,195 - EX28DRAFT_0506 0.65 -0.7
Or see this region's nucleotide sequence