Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0468 and EX28DRAFT_0469 overlap by 4 nucleotides EX28DRAFT_0469 and EX28DRAFT_0470 are separated by 42 nucleotides EX28DRAFT_0470 and EX28DRAFT_0471 overlap by 27 nucleotides
EX28DRAFT_0468: EX28DRAFT_0468 - hydroxyethylthiazole kinase, at 451,654 to 452,424
_0468
EX28DRAFT_0469: EX28DRAFT_0469 - hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase, at 452,421 to 453,221
_0469
EX28DRAFT_0470: EX28DRAFT_0470 - Transcriptional regulators, at 453,264 to 454,010
_0470
EX28DRAFT_0471: EX28DRAFT_0471 - Sugar kinases, ribokinase family, at 453,984 to 454,937
_0471
Position (kb)
452
453
454 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 451.470 kb on - strand at 451.569 kb on + strand at 451.572 kb on + strand at 451.572 kb on + strand at 451.677 kb on - strand at 451.740 kb on - strand, within EX28DRAFT_0468 at 451.857 kb on + strand, within EX28DRAFT_0468 at 451.899 kb on - strand, within EX28DRAFT_0468 at 452.401 kb on + strand at 452.402 kb on - strand at 452.703 kb on - strand, within EX28DRAFT_0469 at 452.806 kb on + strand, within EX28DRAFT_0469 at 453.008 kb on - strand, within EX28DRAFT_0469 at 453.705 kb on + strand, within EX28DRAFT_0470 at 453.798 kb on + strand, within EX28DRAFT_0470 at 453.799 kb on - strand, within EX28DRAFT_0470 at 453.884 kb on + strand, within EX28DRAFT_0470 at 453.884 kb on + strand, within EX28DRAFT_0470 at 453.885 kb on - strand, within EX28DRAFT_0470 at 453.969 kb on + strand at 453.970 kb on - strand at 453.986 kb on - strand at 453.988 kb on - strand at 454.008 kb on + strand at 454.008 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 451,470 - -0.4 451,569 + +0.3 451,572 + +0.1 451,572 + +0.5 451,677 - +0.7 451,740 - EX28DRAFT_0468 0.11 -1.7 451,857 + EX28DRAFT_0468 0.26 -1.2 451,899 - EX28DRAFT_0468 0.32 -0.6 452,401 + -0.4 452,402 - -1.2 452,703 - EX28DRAFT_0469 0.35 -0.7 452,806 + EX28DRAFT_0469 0.48 -3.8 453,008 - EX28DRAFT_0469 0.73 -0.5 453,705 + EX28DRAFT_0470 0.59 -0.2 453,798 + EX28DRAFT_0470 0.71 -0.2 453,799 - EX28DRAFT_0470 0.72 -0.3 453,884 + EX28DRAFT_0470 0.83 +0.1 453,884 + EX28DRAFT_0470 0.83 +0.2 453,885 - EX28DRAFT_0470 0.83 +0.7 453,969 + +0.4 453,970 - +0.2 453,986 - -0.0 453,988 - -0.3 454,008 + +0.0 454,008 + -0.5
Or see this region's nucleotide sequence