Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS04940 and ABIE41_RS04945 are separated by 10 nucleotides ABIE41_RS04945 and ABIE41_RS04950 overlap by 4 nucleotides ABIE41_RS04950 and ABIE41_RS04955 are separated by 183 nucleotides
ABIE41_RS04940: ABIE41_RS04940 - beta-ketoacyl-ACP synthase, at 1,027,525 to 1,028,838
_RS04940
ABIE41_RS04945: ABIE41_RS04945 - beta-ketoacyl-ACP synthase, at 1,028,849 to 1,029,976
_RS04945
ABIE41_RS04950: ABIE41_RS04950 - 3-hydroxyacyl-ACP dehydratase FabZ family protein, at 1,029,973 to 1,030,446
_RS04950
ABIE41_RS04955: ABIE41_RS04955 - acyl carrier protein, at 1,030,630 to 1,030,911
_RS04955
Position (kb) 1028
1029
1030 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1027.878 kb on - strand, within ABIE41_RS04940 at 1028.852 kb on + strand at 1028.852 kb on + strand at 1028.853 kb on - strand at 1029.380 kb on + strand, within ABIE41_RS04945 at 1029.381 kb on + strand, within ABIE41_RS04945 at 1029.381 kb on + strand, within ABIE41_RS04945 at 1029.381 kb on + strand, within ABIE41_RS04945 at 1029.381 kb on + strand, within ABIE41_RS04945 at 1029.382 kb on - strand, within ABIE41_RS04945 at 1029.382 kb on - strand, within ABIE41_RS04945 at 1029.382 kb on - strand, within ABIE41_RS04945 at 1029.382 kb on - strand, within ABIE41_RS04945 at 1029.382 kb on - strand, within ABIE41_RS04945 at 1029.754 kb on - strand, within ABIE41_RS04945 at 1029.914 kb on - strand at 1030.127 kb on + strand, within ABIE41_RS04950 at 1030.262 kb on + strand, within ABIE41_RS04950 at 1030.262 kb on + strand, within ABIE41_RS04950 at 1030.433 kb on + strand at 1030.691 kb on + strand, within ABIE41_RS04955 at 1030.691 kb on + strand, within ABIE41_RS04955
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 1,027,878 - ABIE41_RS04940 0.27 -0.3 1,028,852 + +0.7 1,028,852 + -1.6 1,028,853 - +0.3 1,029,380 + ABIE41_RS04945 0.47 -0.3 1,029,381 + ABIE41_RS04945 0.47 -0.6 1,029,381 + ABIE41_RS04945 0.47 -1.2 1,029,381 + ABIE41_RS04945 0.47 -2.0 1,029,381 + ABIE41_RS04945 0.47 -0.6 1,029,382 - ABIE41_RS04945 0.47 +2.3 1,029,382 - ABIE41_RS04945 0.47 -0.6 1,029,382 - ABIE41_RS04945 0.47 -1.4 1,029,382 - ABIE41_RS04945 0.47 -0.3 1,029,382 - ABIE41_RS04945 0.47 -0.4 1,029,754 - ABIE41_RS04945 0.80 -1.4 1,029,914 - -1.3 1,030,127 + ABIE41_RS04950 0.32 -0.2 1,030,262 + ABIE41_RS04950 0.61 +0.2 1,030,262 + ABIE41_RS04950 0.61 +0.7 1,030,433 + +0.2 1,030,691 + ABIE41_RS04955 0.22 -2.5 1,030,691 + ABIE41_RS04955 0.22 -3.5
Or see this region's nucleotide sequence