Experiment: 4-Hydroxyvalerate (C) (40mM)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0349 and PP_0350 are separated by 56 nucleotides PP_0350 and PP_0351 are separated by 251 nucleotides
PP_0349: PP_0349 - putative Membrane protein, at 424,197 to 425,309
_0349
PP_0350: PP_0350 - Outer membrane ferrichrome-iron receptor, at 425,366 to 427,798
_0350
PP_0351: PP_0351 - putative Transmembrane sensor protein, at 428,050 to 429,006
_0351
Position (kb) 425
426
427
428 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 424.416 kb on + strand, within PP_0349 at 424.417 kb on - strand, within PP_0349 at 424.472 kb on - strand, within PP_0349 at 424.472 kb on - strand, within PP_0349 at 424.472 kb on - strand, within PP_0349 at 424.674 kb on + strand, within PP_0349 at 424.774 kb on - strand, within PP_0349 at 424.810 kb on + strand, within PP_0349 at 424.810 kb on + strand, within PP_0349 at 424.811 kb on - strand, within PP_0349 at 424.848 kb on + strand, within PP_0349 at 424.848 kb on + strand, within PP_0349 at 424.849 kb on - strand, within PP_0349 at 424.849 kb on - strand, within PP_0349 at 425.179 kb on - strand, within PP_0349 at 425.207 kb on + strand at 425.208 kb on - strand at 425.208 kb on - strand at 425.208 kb on - strand at 425.249 kb on + strand at 425.249 kb on + strand at 425.250 kb on - strand at 425.376 kb on + strand at 425.376 kb on + strand at 425.376 kb on + strand at 425.377 kb on - strand at 425.377 kb on - strand at 425.377 kb on - strand at 425.377 kb on - strand at 425.412 kb on + strand at 425.412 kb on + strand at 425.412 kb on + strand at 425.415 kb on + strand at 425.416 kb on - strand at 425.416 kb on - strand at 425.416 kb on - strand at 425.421 kb on + strand at 425.421 kb on + strand at 425.421 kb on + strand at 425.422 kb on - strand at 425.422 kb on - strand at 425.422 kb on - strand at 425.422 kb on - strand at 425.424 kb on + strand at 425.424 kb on + strand at 425.424 kb on + strand at 425.424 kb on + strand at 425.425 kb on - strand at 425.425 kb on - strand at 425.438 kb on - strand at 425.438 kb on - strand at 425.438 kb on - strand at 425.445 kb on + strand at 425.446 kb on - strand at 425.446 kb on - strand at 425.523 kb on + strand at 425.550 kb on + strand at 425.571 kb on + strand at 425.572 kb on - strand at 425.572 kb on - strand at 425.575 kb on + strand at 425.576 kb on - strand at 425.576 kb on - strand at 425.576 kb on - strand at 425.576 kb on - strand at 425.576 kb on - strand at 425.596 kb on + strand at 425.596 kb on + strand at 425.596 kb on + strand at 425.597 kb on - strand at 425.597 kb on - strand at 425.605 kb on + strand at 425.661 kb on + strand, within PP_0350 at 425.661 kb on + strand, within PP_0350 at 425.661 kb on + strand, within PP_0350 at 425.662 kb on - strand, within PP_0350 at 425.754 kb on + strand, within PP_0350 at 425.941 kb on - strand, within PP_0350 at 426.037 kb on - strand, within PP_0350 at 426.073 kb on - strand, within PP_0350 at 426.115 kb on + strand, within PP_0350 at 426.197 kb on - strand, within PP_0350 at 426.197 kb on - strand, within PP_0350 at 426.222 kb on + strand, within PP_0350 at 426.222 kb on + strand, within PP_0350 at 426.222 kb on + strand, within PP_0350 at 426.223 kb on - strand, within PP_0350 at 426.223 kb on - strand, within PP_0350 at 426.223 kb on - strand, within PP_0350 at 426.252 kb on + strand, within PP_0350 at 426.258 kb on + strand, within PP_0350 at 426.354 kb on + strand, within PP_0350 at 426.354 kb on + strand, within PP_0350 at 426.354 kb on + strand, within PP_0350 at 426.354 kb on + strand, within PP_0350 at 426.354 kb on + strand, within PP_0350 at 426.531 kb on + strand, within PP_0350 at 426.531 kb on + strand, within PP_0350 at 426.532 kb on - strand, within PP_0350 at 426.532 kb on - strand, within PP_0350 at 426.532 kb on - strand, within PP_0350 at 426.577 kb on - strand, within PP_0350 at 426.642 kb on + strand, within PP_0350 at 426.642 kb on + strand, within PP_0350 at 426.643 kb on - strand, within PP_0350 at 426.645 kb on + strand, within PP_0350 at 426.646 kb on - strand at 426.658 kb on - strand, within PP_0350 at 426.844 kb on - strand, within PP_0350 at 426.889 kb on + strand, within PP_0350 at 426.908 kb on + strand, within PP_0350 at 427.134 kb on + strand, within PP_0350 at 427.134 kb on + strand, within PP_0350 at 427.134 kb on + strand, within PP_0350 at 427.134 kb on + strand, within PP_0350 at 427.134 kb on + strand, within PP_0350 at 427.134 kb on + strand, within PP_0350 at 427.135 kb on - strand, within PP_0350 at 427.213 kb on + strand, within PP_0350 at 427.249 kb on + strand, within PP_0350 at 427.251 kb on + strand, within PP_0350 at 427.251 kb on + strand, within PP_0350 at 427.251 kb on + strand, within PP_0350 at 427.251 kb on + strand, within PP_0350 at 427.252 kb on - strand, within PP_0350 at 427.252 kb on - strand, within PP_0350 at 427.383 kb on + strand, within PP_0350 at 427.383 kb on + strand, within PP_0350 at 427.384 kb on - strand, within PP_0350 at 427.482 kb on + strand, within PP_0350 at 427.482 kb on + strand, within PP_0350 at 427.482 kb on + strand, within PP_0350 at 427.483 kb on - strand, within PP_0350 at 427.558 kb on - strand at 427.558 kb on - strand at 427.680 kb on + strand at 427.680 kb on + strand at 427.681 kb on - strand at 427.777 kb on + strand at 427.777 kb on + strand at 427.777 kb on + strand at 427.778 kb on - strand at 427.778 kb on - strand at 427.973 kb on + strand at 427.973 kb on + strand at 427.996 kb on - strand at 428.051 kb on + strand at 428.051 kb on + strand at 428.082 kb on + strand at 428.082 kb on + strand at 428.083 kb on - strand at 428.083 kb on - strand at 428.248 kb on + strand, within PP_0351 at 428.248 kb on + strand, within PP_0351 at 428.248 kb on + strand, within PP_0351 at 428.249 kb on - strand, within PP_0351 at 428.249 kb on - strand, within PP_0351 at 428.249 kb on - strand, within PP_0351 at 428.425 kb on - strand, within PP_0351 at 428.492 kb on - strand, within PP_0351 at 428.577 kb on - strand, within PP_0351 at 428.645 kb on - strand, within PP_0351 at 428.645 kb on - strand, within PP_0351 at 428.645 kb on - strand, within PP_0351 at 428.682 kb on - strand, within PP_0351 at 428.682 kb on - strand, within PP_0351
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Hydroxyvalerate (C) (40mM) remove 424,416 + PP_0349 0.20 +0.1 424,417 - PP_0349 0.20 -0.1 424,472 - PP_0349 0.25 -0.3 424,472 - PP_0349 0.25 -0.3 424,472 - PP_0349 0.25 -0.3 424,674 + PP_0349 0.43 +0.7 424,774 - PP_0349 0.52 +0.7 424,810 + PP_0349 0.55 -0.3 424,810 + PP_0349 0.55 -0.9 424,811 - PP_0349 0.55 +0.1 424,848 + PP_0349 0.58 +0.5 424,848 + PP_0349 0.58 +0.3 424,849 - PP_0349 0.59 -0.1 424,849 - PP_0349 0.59 -0.6 425,179 - PP_0349 0.88 -0.3 425,207 + -0.7 425,208 - +0.8 425,208 - +0.1 425,208 - +0.8 425,249 + -0.1 425,249 + -2.3 425,250 - +0.5 425,376 + +0.2 425,376 + -0.4 425,376 + +0.7 425,377 - -0.1 425,377 - -1.0 425,377 - -0.6 425,377 - -0.7 425,412 + -1.2 425,412 + -1.1 425,412 + -0.2 425,415 + +0.4 425,416 - +0.4 425,416 - -0.8 425,416 - +0.2 425,421 + +0.6 425,421 + +0.4 425,421 + -0.1 425,422 - -0.3 425,422 - -0.2 425,422 - -0.0 425,422 - -0.4 425,424 + -0.2 425,424 + -0.3 425,424 + +0.2 425,424 + +0.6 425,425 - -0.1 425,425 - +0.5 425,438 - +1.5 425,438 - +0.5 425,438 - +1.0 425,445 + -0.3 425,446 - +0.5 425,446 - +0.2 425,523 + -0.5 425,550 + -0.3 425,571 + +0.5 425,572 - +0.7 425,572 - +0.3 425,575 + +0.6 425,576 - +0.6 425,576 - +1.3 425,576 - -0.1 425,576 - -0.1 425,576 - +0.5 425,596 + -0.0 425,596 + -0.3 425,596 + +0.7 425,597 - -1.2 425,597 - -1.2 425,605 + +0.5 425,661 + PP_0350 0.12 +1.4 425,661 + PP_0350 0.12 -0.2 425,661 + PP_0350 0.12 +0.3 425,662 - PP_0350 0.12 -0.9 425,754 + PP_0350 0.16 +0.5 425,941 - PP_0350 0.24 -1.2 426,037 - PP_0350 0.28 -0.3 426,073 - PP_0350 0.29 +1.1 426,115 + PP_0350 0.31 +0.3 426,197 - PP_0350 0.34 +0.8 426,197 - PP_0350 0.34 +0.4 426,222 + PP_0350 0.35 +0.6 426,222 + PP_0350 0.35 +0.3 426,222 + PP_0350 0.35 +1.4 426,223 - PP_0350 0.35 +0.1 426,223 - PP_0350 0.35 +0.1 426,223 - PP_0350 0.35 -1.5 426,252 + PP_0350 0.36 +0.2 426,258 + PP_0350 0.37 +0.3 426,354 + PP_0350 0.41 +0.2 426,354 + PP_0350 0.41 -0.0 426,354 + PP_0350 0.41 -0.3 426,354 + PP_0350 0.41 +0.6 426,354 + PP_0350 0.41 +0.1 426,531 + PP_0350 0.48 -0.0 426,531 + PP_0350 0.48 +0.7 426,532 - PP_0350 0.48 -0.8 426,532 - PP_0350 0.48 -0.2 426,532 - PP_0350 0.48 +1.2 426,577 - PP_0350 0.50 +0.1 426,642 + PP_0350 0.52 +0.4 426,642 + PP_0350 0.52 -0.6 426,643 - PP_0350 0.52 +1.1 426,645 + PP_0350 0.53 +0.4 426,646 - +0.4 426,658 - PP_0350 0.53 +0.5 426,844 - PP_0350 0.61 +1.2 426,889 + PP_0350 0.63 -0.2 426,908 + PP_0350 0.63 +0.3 427,134 + PP_0350 0.73 -0.7 427,134 + PP_0350 0.73 +0.6 427,134 + PP_0350 0.73 +0.2 427,134 + PP_0350 0.73 -1.2 427,134 + PP_0350 0.73 +0.0 427,134 + PP_0350 0.73 -0.4 427,135 - PP_0350 0.73 +0.5 427,213 + PP_0350 0.76 +1.1 427,249 + PP_0350 0.77 +0.6 427,251 + PP_0350 0.77 +0.3 427,251 + PP_0350 0.77 -0.1 427,251 + PP_0350 0.77 -0.3 427,251 + PP_0350 0.77 -0.2 427,252 - PP_0350 0.78 +0.5 427,252 - PP_0350 0.78 +1.2 427,383 + PP_0350 0.83 +0.1 427,383 + PP_0350 0.83 -0.4 427,384 - PP_0350 0.83 +1.1 427,482 + PP_0350 0.87 +1.0 427,482 + PP_0350 0.87 -0.5 427,482 + PP_0350 0.87 -0.5 427,483 - PP_0350 0.87 -0.7 427,558 - +3.4 427,558 - -0.7 427,680 + -0.4 427,680 + -0.4 427,681 - +0.0 427,777 + +0.9 427,777 + +0.0 427,777 + -0.6 427,778 - -0.2 427,778 - +1.2 427,973 + +0.8 427,973 + +0.4 427,996 - -0.4 428,051 + -1.4 428,051 + +0.7 428,082 + +0.1 428,082 + +1.0 428,083 - -0.4 428,083 - +0.4 428,248 + PP_0351 0.21 -0.3 428,248 + PP_0351 0.21 -0.5 428,248 + PP_0351 0.21 +0.7 428,249 - PP_0351 0.21 -0.5 428,249 - PP_0351 0.21 +0.1 428,249 - PP_0351 0.21 +0.8 428,425 - PP_0351 0.39 -0.3 428,492 - PP_0351 0.46 -1.6 428,577 - PP_0351 0.55 +2.4 428,645 - PP_0351 0.62 -0.2 428,645 - PP_0351 0.62 -0.1 428,645 - PP_0351 0.62 -0.0 428,682 - PP_0351 0.66 -0.1 428,682 - PP_0351 0.66 +0.1
Or see this region's nucleotide sequence