Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4118 and EX28DRAFT_4119 are separated by 18 nucleotides EX28DRAFT_4119 and EX28DRAFT_4120 are separated by 95 nucleotides
EX28DRAFT_4118: EX28DRAFT_4118 - glycogen/starch synthase, ADP-glucose type, at 91,629 to 93,062
_4118
EX28DRAFT_4119: EX28DRAFT_4119 - glycogen/starch/alpha-glucan phosphorylases, at 93,081 to 95,528
_4119
EX28DRAFT_4120: EX28DRAFT_4120 - Glycerol-3-phosphate dehydrogenase, at 95,624 to 97,132
_4120
Position (kb) 93
94
95
96 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 92.111 kb on + strand, within EX28DRAFT_4118 at 92.133 kb on + strand, within EX28DRAFT_4118 at 92.133 kb on + strand, within EX28DRAFT_4118 at 92.133 kb on + strand, within EX28DRAFT_4118 at 92.133 kb on + strand, within EX28DRAFT_4118 at 92.134 kb on - strand, within EX28DRAFT_4118 at 92.134 kb on - strand, within EX28DRAFT_4118 at 92.134 kb on - strand, within EX28DRAFT_4118 at 92.134 kb on - strand, within EX28DRAFT_4118 at 92.134 kb on - strand, within EX28DRAFT_4118 at 92.134 kb on - strand, within EX28DRAFT_4118 at 92.134 kb on - strand, within EX28DRAFT_4118 at 92.134 kb on - strand, within EX28DRAFT_4118 at 92.136 kb on + strand, within EX28DRAFT_4118 at 92.137 kb on - strand, within EX28DRAFT_4118 at 92.137 kb on - strand, within EX28DRAFT_4118 at 92.179 kb on - strand, within EX28DRAFT_4118 at 92.180 kb on + strand, within EX28DRAFT_4118 at 92.239 kb on - strand, within EX28DRAFT_4118 at 92.249 kb on + strand, within EX28DRAFT_4118 at 92.249 kb on + strand, within EX28DRAFT_4118 at 92.250 kb on - strand, within EX28DRAFT_4118 at 92.250 kb on - strand, within EX28DRAFT_4118 at 92.250 kb on - strand, within EX28DRAFT_4118 at 92.250 kb on - strand, within EX28DRAFT_4118 at 92.272 kb on - strand, within EX28DRAFT_4118 at 92.578 kb on + strand, within EX28DRAFT_4118 at 92.906 kb on + strand, within EX28DRAFT_4118 at 92.907 kb on - strand, within EX28DRAFT_4118 at 93.009 kb on - strand at 93.009 kb on - strand at 93.045 kb on + strand at 93.060 kb on + strand at 93.060 kb on + strand at 93.060 kb on + strand at 93.080 kb on + strand at 93.095 kb on + strand at 93.095 kb on + strand at 93.095 kb on + strand at 93.100 kb on - strand at 93.147 kb on + strand at 93.185 kb on - strand at 93.185 kb on - strand at 93.239 kb on - strand at 93.239 kb on - strand at 93.301 kb on - strand at 93.301 kb on - strand at 93.301 kb on - strand at 93.359 kb on + strand, within EX28DRAFT_4119 at 93.359 kb on + strand, within EX28DRAFT_4119 at 93.360 kb on - strand, within EX28DRAFT_4119 at 93.360 kb on - strand, within EX28DRAFT_4119 at 93.401 kb on - strand, within EX28DRAFT_4119 at 93.401 kb on - strand, within EX28DRAFT_4119 at 93.401 kb on - strand, within EX28DRAFT_4119 at 93.406 kb on + strand, within EX28DRAFT_4119 at 93.406 kb on + strand, within EX28DRAFT_4119 at 93.542 kb on + strand, within EX28DRAFT_4119 at 93.542 kb on + strand, within EX28DRAFT_4119 at 93.542 kb on + strand, within EX28DRAFT_4119 at 93.543 kb on - strand, within EX28DRAFT_4119 at 93.543 kb on - strand, within EX28DRAFT_4119 at 93.591 kb on + strand, within EX28DRAFT_4119 at 93.623 kb on + strand, within EX28DRAFT_4119 at 93.643 kb on - strand, within EX28DRAFT_4119 at 93.834 kb on + strand, within EX28DRAFT_4119 at 93.835 kb on - strand, within EX28DRAFT_4119 at 93.855 kb on - strand, within EX28DRAFT_4119 at 93.906 kb on + strand, within EX28DRAFT_4119 at 93.906 kb on + strand, within EX28DRAFT_4119 at 93.907 kb on - strand, within EX28DRAFT_4119 at 93.907 kb on - strand, within EX28DRAFT_4119 at 94.002 kb on + strand, within EX28DRAFT_4119 at 94.003 kb on - strand, within EX28DRAFT_4119 at 94.098 kb on - strand, within EX28DRAFT_4119 at 94.098 kb on - strand, within EX28DRAFT_4119 at 94.098 kb on - strand, within EX28DRAFT_4119 at 94.138 kb on + strand, within EX28DRAFT_4119 at 94.139 kb on - strand, within EX28DRAFT_4119 at 94.139 kb on - strand, within EX28DRAFT_4119 at 94.317 kb on - strand, within EX28DRAFT_4119 at 94.544 kb on + strand, within EX28DRAFT_4119 at 94.743 kb on + strand, within EX28DRAFT_4119 at 94.743 kb on + strand, within EX28DRAFT_4119 at 94.744 kb on - strand, within EX28DRAFT_4119 at 94.774 kb on + strand, within EX28DRAFT_4119 at 94.774 kb on + strand, within EX28DRAFT_4119 at 94.774 kb on + strand, within EX28DRAFT_4119 at 94.774 kb on + strand, within EX28DRAFT_4119 at 94.775 kb on - strand, within EX28DRAFT_4119 at 94.785 kb on + strand, within EX28DRAFT_4119 at 94.866 kb on + strand, within EX28DRAFT_4119 at 94.866 kb on + strand, within EX28DRAFT_4119 at 94.866 kb on + strand, within EX28DRAFT_4119 at 94.866 kb on + strand, within EX28DRAFT_4119 at 94.866 kb on + strand, within EX28DRAFT_4119 at 94.866 kb on + strand, within EX28DRAFT_4119 at 94.866 kb on + strand, within EX28DRAFT_4119 at 94.867 kb on - strand, within EX28DRAFT_4119 at 94.903 kb on + strand, within EX28DRAFT_4119 at 94.904 kb on - strand, within EX28DRAFT_4119 at 94.904 kb on - strand, within EX28DRAFT_4119 at 94.968 kb on + strand, within EX28DRAFT_4119 at 94.968 kb on + strand, within EX28DRAFT_4119 at 95.058 kb on - strand, within EX28DRAFT_4119 at 95.058 kb on - strand, within EX28DRAFT_4119 at 95.148 kb on - strand, within EX28DRAFT_4119 at 95.166 kb on - strand, within EX28DRAFT_4119 at 95.217 kb on + strand, within EX28DRAFT_4119 at 95.240 kb on - strand, within EX28DRAFT_4119 at 95.277 kb on - strand, within EX28DRAFT_4119 at 95.325 kb on - strand at 95.340 kb on + strand at 95.436 kb on + strand at 95.436 kb on + strand at 95.437 kb on - strand at 95.437 kb on - strand at 95.438 kb on + strand at 95.439 kb on - strand at 95.439 kb on - strand at 95.450 kb on + strand at 95.457 kb on + strand at 95.457 kb on + strand at 95.458 kb on - strand at 95.507 kb on + strand at 95.507 kb on + strand at 95.507 kb on + strand at 95.526 kb on + strand at 95.527 kb on - strand at 95.734 kb on + strand at 96.016 kb on - strand, within EX28DRAFT_4120 at 96.131 kb on - strand, within EX28DRAFT_4120 at 96.131 kb on - strand, within EX28DRAFT_4120 at 96.247 kb on - strand, within EX28DRAFT_4120 at 96.261 kb on + strand, within EX28DRAFT_4120 at 96.310 kb on - strand, within EX28DRAFT_4120
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 92,111 + EX28DRAFT_4118 0.34 -0.0 92,133 + EX28DRAFT_4118 0.35 +0.2 92,133 + EX28DRAFT_4118 0.35 -0.2 92,133 + EX28DRAFT_4118 0.35 -0.5 92,133 + EX28DRAFT_4118 0.35 +0.2 92,134 - EX28DRAFT_4118 0.35 -0.1 92,134 - EX28DRAFT_4118 0.35 -0.2 92,134 - EX28DRAFT_4118 0.35 +0.3 92,134 - EX28DRAFT_4118 0.35 +0.7 92,134 - EX28DRAFT_4118 0.35 +0.1 92,134 - EX28DRAFT_4118 0.35 -1.3 92,134 - EX28DRAFT_4118 0.35 -1.6 92,134 - EX28DRAFT_4118 0.35 +0.3 92,136 + EX28DRAFT_4118 0.35 +0.1 92,137 - EX28DRAFT_4118 0.35 +0.8 92,137 - EX28DRAFT_4118 0.35 -0.6 92,179 - EX28DRAFT_4118 0.38 +0.6 92,180 + EX28DRAFT_4118 0.38 +0.0 92,239 - EX28DRAFT_4118 0.43 +0.1 92,249 + EX28DRAFT_4118 0.43 -2.5 92,249 + EX28DRAFT_4118 0.43 -1.7 92,250 - EX28DRAFT_4118 0.43 +0.2 92,250 - EX28DRAFT_4118 0.43 +0.7 92,250 - EX28DRAFT_4118 0.43 -0.1 92,250 - EX28DRAFT_4118 0.43 +1.2 92,272 - EX28DRAFT_4118 0.45 -0.1 92,578 + EX28DRAFT_4118 0.66 -0.1 92,906 + EX28DRAFT_4118 0.89 +0.4 92,907 - EX28DRAFT_4118 0.89 -1.0 93,009 - +0.7 93,009 - -0.4 93,045 + -0.9 93,060 + +0.6 93,060 + +1.4 93,060 + -1.2 93,080 + +1.3 93,095 + -2.9 93,095 + -0.9 93,095 + +0.9 93,100 - +0.0 93,147 + -0.4 93,185 - -0.7 93,185 - -0.4 93,239 - -1.9 93,239 - +0.1 93,301 - -3.5 93,301 - -1.1 93,301 - -1.2 93,359 + EX28DRAFT_4119 0.11 -0.4 93,359 + EX28DRAFT_4119 0.11 -0.9 93,360 - EX28DRAFT_4119 0.11 -0.3 93,360 - EX28DRAFT_4119 0.11 +0.7 93,401 - EX28DRAFT_4119 0.13 +0.0 93,401 - EX28DRAFT_4119 0.13 +1.4 93,401 - EX28DRAFT_4119 0.13 -0.6 93,406 + EX28DRAFT_4119 0.13 +1.8 93,406 + EX28DRAFT_4119 0.13 -1.4 93,542 + EX28DRAFT_4119 0.19 -0.6 93,542 + EX28DRAFT_4119 0.19 -0.7 93,542 + EX28DRAFT_4119 0.19 +1.1 93,543 - EX28DRAFT_4119 0.19 -0.4 93,543 - EX28DRAFT_4119 0.19 +0.4 93,591 + EX28DRAFT_4119 0.21 +0.0 93,623 + EX28DRAFT_4119 0.22 +0.4 93,643 - EX28DRAFT_4119 0.23 -0.2 93,834 + EX28DRAFT_4119 0.31 -0.2 93,835 - EX28DRAFT_4119 0.31 +0.1 93,855 - EX28DRAFT_4119 0.32 -0.3 93,906 + EX28DRAFT_4119 0.34 -3.4 93,906 + EX28DRAFT_4119 0.34 +0.2 93,907 - EX28DRAFT_4119 0.34 -0.0 93,907 - EX28DRAFT_4119 0.34 -0.4 94,002 + EX28DRAFT_4119 0.38 +2.0 94,003 - EX28DRAFT_4119 0.38 -0.4 94,098 - EX28DRAFT_4119 0.42 +1.5 94,098 - EX28DRAFT_4119 0.42 +1.2 94,098 - EX28DRAFT_4119 0.42 -1.3 94,138 + EX28DRAFT_4119 0.43 +0.4 94,139 - EX28DRAFT_4119 0.43 +0.5 94,139 - EX28DRAFT_4119 0.43 +1.0 94,317 - EX28DRAFT_4119 0.50 -0.2 94,544 + EX28DRAFT_4119 0.60 -0.5 94,743 + EX28DRAFT_4119 0.68 -0.3 94,743 + EX28DRAFT_4119 0.68 +1.6 94,744 - EX28DRAFT_4119 0.68 -0.5 94,774 + EX28DRAFT_4119 0.69 +0.3 94,774 + EX28DRAFT_4119 0.69 -1.1 94,774 + EX28DRAFT_4119 0.69 -0.2 94,774 + EX28DRAFT_4119 0.69 +0.6 94,775 - EX28DRAFT_4119 0.69 -3.2 94,785 + EX28DRAFT_4119 0.70 -0.2 94,866 + EX28DRAFT_4119 0.73 -0.2 94,866 + EX28DRAFT_4119 0.73 -0.7 94,866 + EX28DRAFT_4119 0.73 -0.6 94,866 + EX28DRAFT_4119 0.73 +0.2 94,866 + EX28DRAFT_4119 0.73 -1.2 94,866 + EX28DRAFT_4119 0.73 -0.2 94,866 + EX28DRAFT_4119 0.73 +0.6 94,867 - EX28DRAFT_4119 0.73 -0.4 94,903 + EX28DRAFT_4119 0.74 -0.5 94,904 - EX28DRAFT_4119 0.74 -0.6 94,904 - EX28DRAFT_4119 0.74 +0.1 94,968 + EX28DRAFT_4119 0.77 +0.2 94,968 + EX28DRAFT_4119 0.77 -0.6 95,058 - EX28DRAFT_4119 0.81 -0.5 95,058 - EX28DRAFT_4119 0.81 -0.7 95,148 - EX28DRAFT_4119 0.84 -0.2 95,166 - EX28DRAFT_4119 0.85 -1.6 95,217 + EX28DRAFT_4119 0.87 -0.3 95,240 - EX28DRAFT_4119 0.88 +0.0 95,277 - EX28DRAFT_4119 0.90 -0.4 95,325 - -1.6 95,340 + -0.8 95,436 + -3.4 95,436 + -0.1 95,437 - +0.7 95,437 - -0.2 95,438 + -0.2 95,439 - -0.1 95,439 - -0.0 95,450 + -0.9 95,457 + -0.1 95,457 + -0.0 95,458 - -0.1 95,507 + +1.4 95,507 + +1.4 95,507 + +0.3 95,526 + -0.2 95,527 - -1.1 95,734 + -0.1 96,016 - EX28DRAFT_4120 0.26 -0.8 96,131 - EX28DRAFT_4120 0.34 -0.6 96,131 - EX28DRAFT_4120 0.34 -0.1 96,247 - EX28DRAFT_4120 0.41 +0.4 96,261 + EX28DRAFT_4120 0.42 +0.4 96,310 - EX28DRAFT_4120 0.45 +1.0
Or see this region's nucleotide sequence