Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3986 and EX28DRAFT_3987 are separated by 28 nucleotides EX28DRAFT_3987 and EX28DRAFT_3988 overlap by 1 nucleotides
EX28DRAFT_3986: EX28DRAFT_3986 - DNA gyrase, B subunit, at 193,289 to 195,700
_3986
EX28DRAFT_3987: EX28DRAFT_3987 - DNA replication and repair protein RecF, at 195,729 to 196,802
_3987
EX28DRAFT_3988: EX28DRAFT_3988 - DNA polymerase III, beta subunit, at 196,802 to 197,902
_3988
Position (kb) 195
196
197 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 195.789 kb on + strand at 195.790 kb on - strand at 195.790 kb on - strand at 195.791 kb on + strand at 195.843 kb on + strand, within EX28DRAFT_3987 at 195.901 kb on - strand, within EX28DRAFT_3987 at 196.067 kb on - strand, within EX28DRAFT_3987 at 196.114 kb on + strand, within EX28DRAFT_3987 at 196.341 kb on + strand, within EX28DRAFT_3987 at 196.342 kb on - strand, within EX28DRAFT_3987 at 196.342 kb on - strand, within EX28DRAFT_3987 at 196.342 kb on - strand, within EX28DRAFT_3987 at 196.411 kb on + strand, within EX28DRAFT_3987 at 196.412 kb on - strand, within EX28DRAFT_3987 at 196.437 kb on - strand, within EX28DRAFT_3987 at 196.437 kb on - strand, within EX28DRAFT_3987 at 196.672 kb on + strand, within EX28DRAFT_3987 at 196.672 kb on + strand, within EX28DRAFT_3987 at 196.673 kb on - strand, within EX28DRAFT_3987 at 196.673 kb on - strand, within EX28DRAFT_3987 at 196.673 kb on - strand, within EX28DRAFT_3987 at 196.673 kb on - strand, within EX28DRAFT_3987 at 196.673 kb on - strand, within EX28DRAFT_3987 at 196.727 kb on + strand at 196.804 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 195,789 + -0.6 195,790 - -0.5 195,790 - -0.1 195,791 + -0.2 195,843 + EX28DRAFT_3987 0.11 -0.3 195,901 - EX28DRAFT_3987 0.16 -2.7 196,067 - EX28DRAFT_3987 0.31 -0.5 196,114 + EX28DRAFT_3987 0.36 -1.0 196,341 + EX28DRAFT_3987 0.57 -3.1 196,342 - EX28DRAFT_3987 0.57 -2.6 196,342 - EX28DRAFT_3987 0.57 -1.5 196,342 - EX28DRAFT_3987 0.57 +0.2 196,411 + EX28DRAFT_3987 0.64 -2.1 196,412 - EX28DRAFT_3987 0.64 -0.0 196,437 - EX28DRAFT_3987 0.66 -1.5 196,437 - EX28DRAFT_3987 0.66 +0.7 196,672 + EX28DRAFT_3987 0.88 -1.2 196,672 + EX28DRAFT_3987 0.88 -0.1 196,673 - EX28DRAFT_3987 0.88 +0.2 196,673 - EX28DRAFT_3987 0.88 +0.1 196,673 - EX28DRAFT_3987 0.88 -0.5 196,673 - EX28DRAFT_3987 0.88 -0.2 196,673 - EX28DRAFT_3987 0.88 -0.3 196,727 + -0.1 196,804 - -0.0
Or see this region's nucleotide sequence