Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3889 and EX28DRAFT_3890 overlap by 23 nucleotides EX28DRAFT_3890 and EX28DRAFT_3891 are separated by 120 nucleotides EX28DRAFT_3891 and EX28DRAFT_3892 are separated by 40 nucleotides EX28DRAFT_3892 and EX28DRAFT_3893 are separated by 67 nucleotides
EX28DRAFT_3889: EX28DRAFT_3889 - phosphopantothenoylcysteine decarboxylase / phosphopantothenate--cysteine ligase, at 97,574 to 98,788
_3889
EX28DRAFT_3890: EX28DRAFT_3890 - deoxyuridine 5'-triphosphate nucleotidohydrolase (dut), at 98,766 to 99,224
_3890
EX28DRAFT_3891: EX28DRAFT_3891 - transcriptional regulator, TetR family, at 99,345 to 99,941
_3891
EX28DRAFT_3892: EX28DRAFT_3892 - orotate phosphoribosyltransferase (EC 2.4.2.10), at 99,982 to 100,623
_3892
EX28DRAFT_3893: EX28DRAFT_3893 - ribonuclease PH, at 100,691 to 101,407
_3893
Position (kb) 99
100 Strain fitness (log2 ratio) -2
-1
0
1 at 99.222 kb on + strand at 99.223 kb on - strand at 99.241 kb on + strand at 99.241 kb on + strand at 99.241 kb on + strand at 99.241 kb on + strand at 99.242 kb on - strand at 99.242 kb on - strand at 99.254 kb on + strand at 99.254 kb on + strand at 99.255 kb on - strand at 99.256 kb on + strand at 99.257 kb on - strand at 99.257 kb on - strand at 99.257 kb on - strand at 99.265 kb on - strand at 99.335 kb on - strand at 99.341 kb on + strand at 99.487 kb on + strand, within EX28DRAFT_3891 at 99.489 kb on + strand, within EX28DRAFT_3891 at 99.540 kb on - strand, within EX28DRAFT_3891 at 99.738 kb on - strand, within EX28DRAFT_3891 at 99.870 kb on - strand, within EX28DRAFT_3891 at 99.882 kb on + strand at 99.940 kb on - strand at 99.946 kb on + strand at 99.979 kb on + strand at 99.984 kb on - strand at 100.841 kb on + strand, within EX28DRAFT_3893 at 100.842 kb on - strand, within EX28DRAFT_3893 at 100.913 kb on - strand, within EX28DRAFT_3893
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 99,222 + -0.9 99,223 - +1.3 99,241 + -1.5 99,241 + +0.1 99,241 + -0.8 99,241 + +0.3 99,242 - +0.1 99,242 - +0.4 99,254 + -0.3 99,254 + +0.0 99,255 - -0.7 99,256 + +0.5 99,257 - +0.0 99,257 - +0.8 99,257 - +0.3 99,265 - +0.6 99,335 - +0.0 99,341 + +0.4 99,487 + EX28DRAFT_3891 0.24 -0.0 99,489 + EX28DRAFT_3891 0.24 -0.4 99,540 - EX28DRAFT_3891 0.33 +0.5 99,738 - EX28DRAFT_3891 0.66 -1.6 99,870 - EX28DRAFT_3891 0.88 -1.9 99,882 + +1.2 99,940 - +1.5 99,946 + -0.4 99,979 + +0.3 99,984 - +0.7 100,841 + EX28DRAFT_3893 0.21 -0.1 100,842 - EX28DRAFT_3893 0.21 -1.5 100,913 - EX28DRAFT_3893 0.31 -2.3
Or see this region's nucleotide sequence