Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3547 and EX28DRAFT_3548 are separated by 270 nucleotides EX28DRAFT_3548 and EX28DRAFT_3549 are separated by 83 nucleotides EX28DRAFT_3549 and EX28DRAFT_3550 are separated by 92 nucleotides EX28DRAFT_3550 and EX28DRAFT_3551 overlap by 1 nucleotides
EX28DRAFT_3547: EX28DRAFT_3547 - 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase, C-terminal subunit/4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase, N-terminal subunit, at 210,995 to 212,272
_3547
EX28DRAFT_3548: EX28DRAFT_3548 - homoprotocatechuate degradation operon regulator, HpaR, at 212,543 to 212,983
_3548
EX28DRAFT_3549: EX28DRAFT_3549 - methyl-accepting chemotaxis sensory transducer with TarH sensor, at 213,067 to 214,731
_3549
EX28DRAFT_3550: EX28DRAFT_3550 - Predicted acetyltransferase, at 214,824 to 215,360
_3550
EX28DRAFT_3551: EX28DRAFT_3551 - hypothetical protein, at 215,360 to 215,833
_3551
Position (kb) 213
214
215 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 212.159 kb on - strand at 212.190 kb on + strand at 212.190 kb on + strand at 212.278 kb on + strand at 212.278 kb on + strand at 212.278 kb on + strand at 212.278 kb on + strand at 212.279 kb on - strand at 212.279 kb on - strand at 212.804 kb on + strand, within EX28DRAFT_3548 at 212.804 kb on + strand, within EX28DRAFT_3548 at 212.805 kb on - strand, within EX28DRAFT_3548 at 212.838 kb on - strand, within EX28DRAFT_3548 at 212.938 kb on + strand, within EX28DRAFT_3548 at 212.939 kb on - strand, within EX28DRAFT_3548 at 213.071 kb on + strand at 213.071 kb on + strand at 213.071 kb on + strand at 213.116 kb on + strand at 213.128 kb on + strand at 213.129 kb on - strand at 213.261 kb on + strand, within EX28DRAFT_3549 at 213.261 kb on + strand, within EX28DRAFT_3549 at 213.287 kb on - strand, within EX28DRAFT_3549 at 213.351 kb on + strand, within EX28DRAFT_3549 at 213.351 kb on + strand, within EX28DRAFT_3549 at 213.351 kb on + strand, within EX28DRAFT_3549 at 213.388 kb on + strand, within EX28DRAFT_3549 at 213.447 kb on + strand, within EX28DRAFT_3549 at 213.448 kb on - strand, within EX28DRAFT_3549 at 213.451 kb on + strand, within EX28DRAFT_3549 at 213.451 kb on + strand, within EX28DRAFT_3549 at 213.451 kb on + strand, within EX28DRAFT_3549 at 213.452 kb on - strand, within EX28DRAFT_3549 at 213.457 kb on - strand, within EX28DRAFT_3549 at 213.457 kb on - strand, within EX28DRAFT_3549 at 213.457 kb on - strand, within EX28DRAFT_3549 at 213.541 kb on + strand, within EX28DRAFT_3549 at 213.565 kb on + strand, within EX28DRAFT_3549 at 213.565 kb on + strand, within EX28DRAFT_3549 at 213.565 kb on + strand, within EX28DRAFT_3549 at 213.565 kb on + strand, within EX28DRAFT_3549 at 213.574 kb on + strand, within EX28DRAFT_3549 at 213.642 kb on + strand, within EX28DRAFT_3549 at 213.840 kb on + strand, within EX28DRAFT_3549 at 213.880 kb on - strand, within EX28DRAFT_3549 at 213.898 kb on + strand, within EX28DRAFT_3549 at 213.899 kb on - strand, within EX28DRAFT_3549 at 213.948 kb on + strand, within EX28DRAFT_3549 at 214.355 kb on + strand, within EX28DRAFT_3549 at 214.356 kb on - strand, within EX28DRAFT_3549 at 214.823 kb on - strand at 214.826 kb on - strand at 214.965 kb on + strand, within EX28DRAFT_3550 at 214.966 kb on - strand, within EX28DRAFT_3550 at 215.009 kb on + strand, within EX28DRAFT_3550 at 215.093 kb on + strand, within EX28DRAFT_3550 at 215.167 kb on - strand, within EX28DRAFT_3550 at 215.167 kb on - strand, within EX28DRAFT_3550 at 215.340 kb on + strand at 215.340 kb on + strand at 215.341 kb on - strand at 215.362 kb on - strand at 215.473 kb on - strand, within EX28DRAFT_3551 at 215.569 kb on + strand, within EX28DRAFT_3551 at 215.665 kb on + strand, within EX28DRAFT_3551
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 212,159 - +0.5 212,190 + +0.5 212,190 + +0.6 212,278 + +0.6 212,278 + -0.6 212,278 + +0.3 212,278 + +0.8 212,279 - +0.4 212,279 - +3.1 212,804 + EX28DRAFT_3548 0.59 +0.0 212,804 + EX28DRAFT_3548 0.59 -2.4 212,805 - EX28DRAFT_3548 0.59 +0.7 212,838 - EX28DRAFT_3548 0.67 -0.4 212,938 + EX28DRAFT_3548 0.90 +0.2 212,939 - EX28DRAFT_3548 0.90 -0.3 213,071 + -0.1 213,071 + -0.1 213,071 + +0.2 213,116 + +0.4 213,128 + -0.6 213,129 - +0.2 213,261 + EX28DRAFT_3549 0.12 -1.2 213,261 + EX28DRAFT_3549 0.12 -1.5 213,287 - EX28DRAFT_3549 0.13 +0.3 213,351 + EX28DRAFT_3549 0.17 -0.9 213,351 + EX28DRAFT_3549 0.17 -0.3 213,351 + EX28DRAFT_3549 0.17 -0.1 213,388 + EX28DRAFT_3549 0.19 +0.2 213,447 + EX28DRAFT_3549 0.23 +1.6 213,448 - EX28DRAFT_3549 0.23 +0.0 213,451 + EX28DRAFT_3549 0.23 +0.2 213,451 + EX28DRAFT_3549 0.23 +1.4 213,451 + EX28DRAFT_3549 0.23 -0.0 213,452 - EX28DRAFT_3549 0.23 +0.4 213,457 - EX28DRAFT_3549 0.23 +0.4 213,457 - EX28DRAFT_3549 0.23 +0.3 213,457 - EX28DRAFT_3549 0.23 -0.5 213,541 + EX28DRAFT_3549 0.28 -0.3 213,565 + EX28DRAFT_3549 0.30 -0.0 213,565 + EX28DRAFT_3549 0.30 -0.4 213,565 + EX28DRAFT_3549 0.30 -0.3 213,565 + EX28DRAFT_3549 0.30 -0.7 213,574 + EX28DRAFT_3549 0.30 +2.8 213,642 + EX28DRAFT_3549 0.35 -1.3 213,840 + EX28DRAFT_3549 0.46 -0.3 213,880 - EX28DRAFT_3549 0.49 -0.8 213,898 + EX28DRAFT_3549 0.50 +1.0 213,899 - EX28DRAFT_3549 0.50 +0.9 213,948 + EX28DRAFT_3549 0.53 -1.7 214,355 + EX28DRAFT_3549 0.77 +0.3 214,356 - EX28DRAFT_3549 0.77 +0.6 214,823 - -0.2 214,826 - -0.2 214,965 + EX28DRAFT_3550 0.26 +0.3 214,966 - EX28DRAFT_3550 0.26 +0.7 215,009 + EX28DRAFT_3550 0.34 +0.5 215,093 + EX28DRAFT_3550 0.50 +1.6 215,167 - EX28DRAFT_3550 0.64 -1.3 215,167 - EX28DRAFT_3550 0.64 -0.3 215,340 + -1.3 215,340 + +0.8 215,341 - +0.2 215,362 - +0.1 215,473 - EX28DRAFT_3551 0.24 +0.6 215,569 + EX28DRAFT_3551 0.44 -0.5 215,665 + EX28DRAFT_3551 0.64 -0.1
Or see this region's nucleotide sequence