Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3499 and EX28DRAFT_3500 overlap by 4 nucleotides EX28DRAFT_3500 and EX28DRAFT_3501 overlap by 1 nucleotides EX28DRAFT_3501 and EX28DRAFT_3502 are separated by 218 nucleotides EX28DRAFT_3502 and EX28DRAFT_3503 are separated by 61 nucleotides
EX28DRAFT_3499: EX28DRAFT_3499 - Arabinose efflux permease, at 161,966 to 163,585
_3499
EX28DRAFT_3500: EX28DRAFT_3500 - Predicted membrane protein, at 163,582 to 163,989
_3500
EX28DRAFT_3501: EX28DRAFT_3501 - Predicted metal-dependent hydrolase with the TIM-barrel fold, at 163,989 to 165,860
_3501
EX28DRAFT_3502: EX28DRAFT_3502 - Amidases related to nicotinamidase, at 166,079 to 166,765
_3502
EX28DRAFT_3503: EX28DRAFT_3503 - XapX domain, at 166,827 to 167,048
_3503
Position (kb) 163
164
165
166 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 163.299 kb on + strand, within EX28DRAFT_3499 at 163.300 kb on - strand, within EX28DRAFT_3499 at 163.300 kb on - strand, within EX28DRAFT_3499 at 163.303 kb on + strand, within EX28DRAFT_3499 at 163.303 kb on + strand, within EX28DRAFT_3499 at 163.303 kb on + strand, within EX28DRAFT_3499 at 163.304 kb on - strand, within EX28DRAFT_3499 at 163.304 kb on - strand, within EX28DRAFT_3499 at 163.366 kb on + strand, within EX28DRAFT_3499 at 163.767 kb on - strand, within EX28DRAFT_3500 at 163.767 kb on - strand, within EX28DRAFT_3500 at 163.910 kb on + strand, within EX28DRAFT_3500 at 163.910 kb on + strand, within EX28DRAFT_3500 at 163.911 kb on - strand, within EX28DRAFT_3500 at 164.320 kb on + strand, within EX28DRAFT_3501 at 164.321 kb on - strand, within EX28DRAFT_3501 at 164.363 kb on + strand, within EX28DRAFT_3501 at 164.363 kb on + strand, within EX28DRAFT_3501 at 164.461 kb on + strand, within EX28DRAFT_3501 at 164.461 kb on + strand, within EX28DRAFT_3501 at 164.462 kb on - strand, within EX28DRAFT_3501 at 164.524 kb on + strand, within EX28DRAFT_3501 at 164.638 kb on + strand, within EX28DRAFT_3501 at 164.639 kb on - strand, within EX28DRAFT_3501 at 164.764 kb on - strand, within EX28DRAFT_3501 at 164.764 kb on - strand, within EX28DRAFT_3501 at 165.060 kb on - strand, within EX28DRAFT_3501 at 165.124 kb on + strand, within EX28DRAFT_3501 at 165.412 kb on + strand, within EX28DRAFT_3501 at 165.619 kb on + strand, within EX28DRAFT_3501 at 165.620 kb on - strand, within EX28DRAFT_3501 at 165.861 kb on - strand at 165.926 kb on + strand at 165.928 kb on + strand at 165.928 kb on + strand at 165.929 kb on - strand at 165.929 kb on - strand at 165.929 kb on - strand at 165.933 kb on + strand at 165.933 kb on + strand at 165.943 kb on - strand at 165.943 kb on - strand at 165.944 kb on + strand at 165.952 kb on + strand at 165.953 kb on - strand at 165.953 kb on - strand at 165.954 kb on + strand at 165.955 kb on - strand at 165.955 kb on - strand at 165.958 kb on + strand at 165.959 kb on - strand at 165.959 kb on - strand at 165.959 kb on - strand at 165.959 kb on - strand at 165.959 kb on - strand at 165.960 kb on + strand at 165.961 kb on - strand at 165.961 kb on - strand at 165.965 kb on - strand at 165.974 kb on + strand at 165.975 kb on - strand at 165.975 kb on - strand at 165.975 kb on - strand at 165.986 kb on + strand at 165.987 kb on - strand at 165.990 kb on + strand at 166.078 kb on + strand at 166.079 kb on - strand at 166.171 kb on + strand, within EX28DRAFT_3502 at 166.172 kb on - strand, within EX28DRAFT_3502 at 166.286 kb on + strand, within EX28DRAFT_3502 at 166.333 kb on + strand, within EX28DRAFT_3502 at 166.334 kb on - strand, within EX28DRAFT_3502 at 166.472 kb on + strand, within EX28DRAFT_3502 at 166.482 kb on - strand, within EX28DRAFT_3502 at 166.482 kb on - strand, within EX28DRAFT_3502 at 166.483 kb on + strand, within EX28DRAFT_3502 at 166.484 kb on - strand, within EX28DRAFT_3502 at 166.625 kb on + strand, within EX28DRAFT_3502 at 166.686 kb on - strand, within EX28DRAFT_3502 at 166.686 kb on - strand, within EX28DRAFT_3502 at 166.691 kb on + strand, within EX28DRAFT_3502 at 166.691 kb on + strand, within EX28DRAFT_3502
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 163,299 + EX28DRAFT_3499 0.82 +1.5 163,300 - EX28DRAFT_3499 0.82 +0.4 163,300 - EX28DRAFT_3499 0.82 +0.1 163,303 + EX28DRAFT_3499 0.83 -0.1 163,303 + EX28DRAFT_3499 0.83 +0.5 163,303 + EX28DRAFT_3499 0.83 +0.1 163,304 - EX28DRAFT_3499 0.83 -0.1 163,304 - EX28DRAFT_3499 0.83 +0.5 163,366 + EX28DRAFT_3499 0.86 +0.4 163,767 - EX28DRAFT_3500 0.45 -0.5 163,767 - EX28DRAFT_3500 0.45 -0.3 163,910 + EX28DRAFT_3500 0.80 -0.6 163,910 + EX28DRAFT_3500 0.80 +0.5 163,911 - EX28DRAFT_3500 0.81 -0.5 164,320 + EX28DRAFT_3501 0.18 +0.6 164,321 - EX28DRAFT_3501 0.18 -0.2 164,363 + EX28DRAFT_3501 0.20 -0.7 164,363 + EX28DRAFT_3501 0.20 +0.4 164,461 + EX28DRAFT_3501 0.25 -1.0 164,461 + EX28DRAFT_3501 0.25 +0.9 164,462 - EX28DRAFT_3501 0.25 -0.7 164,524 + EX28DRAFT_3501 0.29 -0.1 164,638 + EX28DRAFT_3501 0.35 +0.0 164,639 - EX28DRAFT_3501 0.35 -0.6 164,764 - EX28DRAFT_3501 0.41 +0.2 164,764 - EX28DRAFT_3501 0.41 -1.1 165,060 - EX28DRAFT_3501 0.57 -0.7 165,124 + EX28DRAFT_3501 0.61 -1.4 165,412 + EX28DRAFT_3501 0.76 +1.4 165,619 + EX28DRAFT_3501 0.87 +0.1 165,620 - EX28DRAFT_3501 0.87 +0.4 165,861 - -0.4 165,926 + -0.1 165,928 + -0.5 165,928 + +0.5 165,929 - +0.1 165,929 - -0.0 165,929 - -2.5 165,933 + +0.2 165,933 + -0.5 165,943 - +0.3 165,943 - +0.5 165,944 + -0.6 165,952 + +0.0 165,953 - -0.4 165,953 - -0.5 165,954 + +2.4 165,955 - +0.5 165,955 - +0.3 165,958 + +0.4 165,959 - -0.7 165,959 - +0.5 165,959 - -0.2 165,959 - -0.2 165,959 - +0.1 165,960 + +0.4 165,961 - +0.3 165,961 - -0.0 165,965 - -0.7 165,974 + +0.4 165,975 - -0.3 165,975 - +0.4 165,975 - -0.6 165,986 + +0.1 165,987 - +1.2 165,990 + -0.2 166,078 + -0.9 166,079 - -0.5 166,171 + EX28DRAFT_3502 0.13 -3.3 166,172 - EX28DRAFT_3502 0.14 -0.3 166,286 + EX28DRAFT_3502 0.30 +0.6 166,333 + EX28DRAFT_3502 0.37 +0.1 166,334 - EX28DRAFT_3502 0.37 +0.3 166,472 + EX28DRAFT_3502 0.57 -0.3 166,482 - EX28DRAFT_3502 0.59 +0.7 166,482 - EX28DRAFT_3502 0.59 +0.3 166,483 + EX28DRAFT_3502 0.59 -1.7 166,484 - EX28DRAFT_3502 0.59 -0.3 166,625 + EX28DRAFT_3502 0.79 +0.2 166,686 - EX28DRAFT_3502 0.88 -0.5 166,686 - EX28DRAFT_3502 0.88 -0.7 166,691 + EX28DRAFT_3502 0.89 -0.7 166,691 + EX28DRAFT_3502 0.89 +2.6
Or see this region's nucleotide sequence