Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3192 and EX28DRAFT_3193 are separated by 12 nucleotides EX28DRAFT_3193 and EX28DRAFT_3194 are separated by 20 nucleotides EX28DRAFT_3194 and EX28DRAFT_3195 are separated by 67 nucleotides EX28DRAFT_3195 and EX28DRAFT_3196 are separated by 62 nucleotides
EX28DRAFT_3192: EX28DRAFT_3192 - putative sugar isomerase, AgaS family, at 492,389 to 493,537
_3192
EX28DRAFT_3193: EX28DRAFT_3193 - class II aldolase, tagatose bisphosphate family, at 493,550 to 494,422
_3193
EX28DRAFT_3194: EX28DRAFT_3194 - hypothetical protein, at 494,443 to 496,332
_3194
EX28DRAFT_3195: EX28DRAFT_3195 - 16S rRNA (cytidine(1402)-2'-O)-methyltransferase, at 496,400 to 497,263
_3195
EX28DRAFT_3196: EX28DRAFT_3196 - LppC putative lipoprotein, at 497,326 to 499,488
_3196
Position (kb)
494
495
496
497 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 493.452 kb on + strand at 493.459 kb on + strand at 493.535 kb on + strand at 493.549 kb on + strand at 493.550 kb on - strand at 493.550 kb on - strand at 493.555 kb on + strand at 493.555 kb on + strand at 493.555 kb on + strand at 493.556 kb on - strand at 493.558 kb on + strand at 493.558 kb on + strand at 493.559 kb on - strand at 493.571 kb on + strand at 493.572 kb on - strand at 493.572 kb on - strand at 493.712 kb on - strand, within EX28DRAFT_3193 at 493.718 kb on - strand, within EX28DRAFT_3193 at 493.718 kb on - strand, within EX28DRAFT_3193 at 493.736 kb on + strand, within EX28DRAFT_3193 at 493.736 kb on + strand, within EX28DRAFT_3193 at 493.736 kb on + strand, within EX28DRAFT_3193 at 494.099 kb on - strand, within EX28DRAFT_3193 at 494.324 kb on + strand, within EX28DRAFT_3193 at 494.324 kb on + strand, within EX28DRAFT_3193 at 494.324 kb on + strand, within EX28DRAFT_3193 at 494.324 kb on + strand, within EX28DRAFT_3193 at 494.324 kb on + strand, within EX28DRAFT_3193 at 494.325 kb on - strand, within EX28DRAFT_3193 at 494.325 kb on - strand, within EX28DRAFT_3193 at 494.325 kb on - strand, within EX28DRAFT_3193 at 494.436 kb on + strand at 494.436 kb on + strand at 494.529 kb on + strand at 494.530 kb on - strand at 494.587 kb on - strand at 494.729 kb on + strand, within EX28DRAFT_3194 at 494.761 kb on + strand, within EX28DRAFT_3194 at 494.761 kb on + strand, within EX28DRAFT_3194 at 494.761 kb on + strand, within EX28DRAFT_3194 at 494.761 kb on + strand, within EX28DRAFT_3194 at 494.762 kb on - strand, within EX28DRAFT_3194 at 494.777 kb on - strand, within EX28DRAFT_3194 at 494.821 kb on - strand, within EX28DRAFT_3194 at 494.857 kb on + strand, within EX28DRAFT_3194 at 494.858 kb on - strand, within EX28DRAFT_3194 at 494.858 kb on - strand, within EX28DRAFT_3194 at 494.862 kb on - strand, within EX28DRAFT_3194 at 494.938 kb on + strand, within EX28DRAFT_3194 at 494.938 kb on + strand, within EX28DRAFT_3194 at 494.938 kb on + strand, within EX28DRAFT_3194 at 494.939 kb on - strand, within EX28DRAFT_3194 at 494.939 kb on - strand, within EX28DRAFT_3194 at 494.939 kb on - strand, within EX28DRAFT_3194 at 494.970 kb on - strand, within EX28DRAFT_3194 at 494.970 kb on - strand, within EX28DRAFT_3194 at 494.970 kb on - strand, within EX28DRAFT_3194 at 494.970 kb on - strand, within EX28DRAFT_3194 at 495.012 kb on + strand, within EX28DRAFT_3194 at 495.099 kb on + strand, within EX28DRAFT_3194 at 495.099 kb on + strand, within EX28DRAFT_3194 at 495.100 kb on - strand, within EX28DRAFT_3194 at 495.100 kb on - strand, within EX28DRAFT_3194 at 495.106 kb on + strand, within EX28DRAFT_3194 at 495.106 kb on + strand, within EX28DRAFT_3194 at 495.110 kb on + strand, within EX28DRAFT_3194 at 495.111 kb on - strand, within EX28DRAFT_3194 at 495.193 kb on - strand, within EX28DRAFT_3194 at 495.373 kb on + strand, within EX28DRAFT_3194 at 495.375 kb on + strand, within EX28DRAFT_3194 at 495.376 kb on - strand, within EX28DRAFT_3194 at 495.463 kb on + strand, within EX28DRAFT_3194 at 495.469 kb on - strand, within EX28DRAFT_3194 at 495.498 kb on - strand, within EX28DRAFT_3194 at 495.516 kb on + strand, within EX28DRAFT_3194 at 495.517 kb on - strand, within EX28DRAFT_3194 at 495.558 kb on + strand, within EX28DRAFT_3194 at 495.561 kb on + strand, within EX28DRAFT_3194 at 495.642 kb on + strand, within EX28DRAFT_3194 at 495.642 kb on + strand, within EX28DRAFT_3194 at 495.643 kb on - strand, within EX28DRAFT_3194 at 495.688 kb on + strand, within EX28DRAFT_3194 at 495.689 kb on - strand, within EX28DRAFT_3194 at 495.730 kb on + strand, within EX28DRAFT_3194 at 495.785 kb on - strand, within EX28DRAFT_3194 at 495.955 kb on + strand, within EX28DRAFT_3194 at 495.955 kb on + strand, within EX28DRAFT_3194 at 495.955 kb on + strand, within EX28DRAFT_3194 at 495.955 kb on + strand, within EX28DRAFT_3194 at 495.955 kb on + strand, within EX28DRAFT_3194 at 495.955 kb on + strand, within EX28DRAFT_3194 at 495.956 kb on - strand, within EX28DRAFT_3194 at 495.956 kb on - strand, within EX28DRAFT_3194 at 495.956 kb on - strand, within EX28DRAFT_3194 at 495.956 kb on - strand, within EX28DRAFT_3194 at 495.956 kb on - strand, within EX28DRAFT_3194 at 495.957 kb on + strand, within EX28DRAFT_3194 at 495.957 kb on + strand, within EX28DRAFT_3194 at 495.957 kb on + strand, within EX28DRAFT_3194 at 495.957 kb on + strand, within EX28DRAFT_3194 at 495.957 kb on + strand, within EX28DRAFT_3194 at 495.958 kb on - strand, within EX28DRAFT_3194 at 496.160 kb on - strand at 496.163 kb on - strand at 496.173 kb on - strand at 496.232 kb on - strand at 496.232 kb on - strand at 496.240 kb on - strand at 496.263 kb on + strand at 496.282 kb on - strand at 496.326 kb on + strand at 496.326 kb on + strand at 496.342 kb on - strand at 496.425 kb on + strand at 496.429 kb on + strand at 496.429 kb on + strand at 496.430 kb on - strand at 496.430 kb on - strand at 496.430 kb on - strand at 496.431 kb on + strand at 496.432 kb on - strand at 496.558 kb on + strand, within EX28DRAFT_3195 at 496.558 kb on + strand, within EX28DRAFT_3195 at 496.558 kb on + strand, within EX28DRAFT_3195 at 496.721 kb on - strand, within EX28DRAFT_3195 at 496.721 kb on - strand, within EX28DRAFT_3195 at 496.796 kb on + strand, within EX28DRAFT_3195 at 496.796 kb on + strand, within EX28DRAFT_3195 at 496.942 kb on + strand, within EX28DRAFT_3195 at 496.942 kb on + strand, within EX28DRAFT_3195 at 496.943 kb on - strand, within EX28DRAFT_3195 at 497.213 kb on + strand at 497.213 kb on + strand at 497.220 kb on + strand at 497.220 kb on + strand at 497.220 kb on + strand at 497.221 kb on - strand at 497.240 kb on - strand at 497.276 kb on - strand at 497.286 kb on + strand at 497.331 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 493,452 + -0.4 493,459 + -0.2 493,535 + -1.3 493,549 + +0.1 493,550 - +1.1 493,550 - +0.4 493,555 + -0.0 493,555 + -0.1 493,555 + -2.0 493,556 - -0.4 493,558 + -0.5 493,558 + -0.7 493,559 - +0.1 493,571 + -1.1 493,572 - -2.4 493,572 - +3.1 493,712 - EX28DRAFT_3193 0.19 +1.0 493,718 - EX28DRAFT_3193 0.19 +0.3 493,718 - EX28DRAFT_3193 0.19 -0.1 493,736 + EX28DRAFT_3193 0.21 -0.2 493,736 + EX28DRAFT_3193 0.21 +0.3 493,736 + EX28DRAFT_3193 0.21 -1.2 494,099 - EX28DRAFT_3193 0.63 -1.7 494,324 + EX28DRAFT_3193 0.89 -0.4 494,324 + EX28DRAFT_3193 0.89 +1.6 494,324 + EX28DRAFT_3193 0.89 -0.1 494,324 + EX28DRAFT_3193 0.89 +0.9 494,324 + EX28DRAFT_3193 0.89 +0.3 494,325 - EX28DRAFT_3193 0.89 +2.8 494,325 - EX28DRAFT_3193 0.89 -0.5 494,325 - EX28DRAFT_3193 0.89 +0.3 494,436 + -0.5 494,436 + -1.6 494,529 + +0.3 494,530 - -1.0 494,587 - -0.0 494,729 + EX28DRAFT_3194 0.15 -1.0 494,761 + EX28DRAFT_3194 0.17 +0.3 494,761 + EX28DRAFT_3194 0.17 -0.3 494,761 + EX28DRAFT_3194 0.17 +0.1 494,761 + EX28DRAFT_3194 0.17 -0.2 494,762 - EX28DRAFT_3194 0.17 +0.5 494,777 - EX28DRAFT_3194 0.18 +0.0 494,821 - EX28DRAFT_3194 0.20 -1.7 494,857 + EX28DRAFT_3194 0.22 -0.6 494,858 - EX28DRAFT_3194 0.22 +0.4 494,858 - EX28DRAFT_3194 0.22 +0.4 494,862 - EX28DRAFT_3194 0.22 -0.1 494,938 + EX28DRAFT_3194 0.26 -0.3 494,938 + EX28DRAFT_3194 0.26 +0.1 494,938 + EX28DRAFT_3194 0.26 +0.1 494,939 - EX28DRAFT_3194 0.26 -1.0 494,939 - EX28DRAFT_3194 0.26 -2.3 494,939 - EX28DRAFT_3194 0.26 -1.6 494,970 - EX28DRAFT_3194 0.28 +0.1 494,970 - EX28DRAFT_3194 0.28 +0.1 494,970 - EX28DRAFT_3194 0.28 -0.8 494,970 - EX28DRAFT_3194 0.28 +0.2 495,012 + EX28DRAFT_3194 0.30 +0.1 495,099 + EX28DRAFT_3194 0.35 -2.5 495,099 + EX28DRAFT_3194 0.35 +1.6 495,100 - EX28DRAFT_3194 0.35 -1.3 495,100 - EX28DRAFT_3194 0.35 -2.3 495,106 + EX28DRAFT_3194 0.35 +0.2 495,106 + EX28DRAFT_3194 0.35 -0.5 495,110 + EX28DRAFT_3194 0.35 +0.5 495,111 - EX28DRAFT_3194 0.35 -0.8 495,193 - EX28DRAFT_3194 0.40 -2.5 495,373 + EX28DRAFT_3194 0.49 +0.3 495,375 + EX28DRAFT_3194 0.49 -0.1 495,376 - EX28DRAFT_3194 0.49 -0.2 495,463 + EX28DRAFT_3194 0.54 -0.0 495,469 - EX28DRAFT_3194 0.54 -1.4 495,498 - EX28DRAFT_3194 0.56 -0.7 495,516 + EX28DRAFT_3194 0.57 +0.1 495,517 - EX28DRAFT_3194 0.57 +0.5 495,558 + EX28DRAFT_3194 0.59 -0.7 495,561 + EX28DRAFT_3194 0.59 -0.8 495,642 + EX28DRAFT_3194 0.63 -0.6 495,642 + EX28DRAFT_3194 0.63 +1.5 495,643 - EX28DRAFT_3194 0.63 -4.0 495,688 + EX28DRAFT_3194 0.66 +0.1 495,689 - EX28DRAFT_3194 0.66 +0.1 495,730 + EX28DRAFT_3194 0.68 +0.3 495,785 - EX28DRAFT_3194 0.71 -0.3 495,955 + EX28DRAFT_3194 0.80 -0.1 495,955 + EX28DRAFT_3194 0.80 +0.6 495,955 + EX28DRAFT_3194 0.80 -0.2 495,955 + EX28DRAFT_3194 0.80 -0.4 495,955 + EX28DRAFT_3194 0.80 +0.0 495,955 + EX28DRAFT_3194 0.80 -1.0 495,956 - EX28DRAFT_3194 0.80 -0.9 495,956 - EX28DRAFT_3194 0.80 +1.6 495,956 - EX28DRAFT_3194 0.80 +0.9 495,956 - EX28DRAFT_3194 0.80 -0.2 495,956 - EX28DRAFT_3194 0.80 +0.2 495,957 + EX28DRAFT_3194 0.80 +0.5 495,957 + EX28DRAFT_3194 0.80 -0.2 495,957 + EX28DRAFT_3194 0.80 +1.4 495,957 + EX28DRAFT_3194 0.80 +1.0 495,957 + EX28DRAFT_3194 0.80 +0.7 495,958 - EX28DRAFT_3194 0.80 +4.4 496,160 - -0.3 496,163 - -0.8 496,173 - +0.9 496,232 - -0.6 496,232 - +0.5 496,240 - +0.5 496,263 + -0.5 496,282 - -1.3 496,326 + -0.2 496,326 + +0.0 496,342 - -0.7 496,425 + +0.4 496,429 + -0.1 496,429 + -0.2 496,430 - +1.0 496,430 - +0.3 496,430 - +0.7 496,431 + -0.4 496,432 - -1.0 496,558 + EX28DRAFT_3195 0.18 +0.8 496,558 + EX28DRAFT_3195 0.18 +0.6 496,558 + EX28DRAFT_3195 0.18 +3.6 496,721 - EX28DRAFT_3195 0.37 -0.5 496,721 - EX28DRAFT_3195 0.37 -1.3 496,796 + EX28DRAFT_3195 0.46 +1.4 496,796 + EX28DRAFT_3195 0.46 -0.1 496,942 + EX28DRAFT_3195 0.63 +2.0 496,942 + EX28DRAFT_3195 0.63 +1.3 496,943 - EX28DRAFT_3195 0.63 +0.7 497,213 + +0.0 497,213 + -0.9 497,220 + +0.2 497,220 + -0.1 497,220 + +1.2 497,221 - +0.0 497,240 - +0.3 497,276 - +1.4 497,286 + +0.1 497,331 - -0.2
Or see this region's nucleotide sequence