Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3179 and EX28DRAFT_3180 are separated by 585 nucleotides EX28DRAFT_3180 and EX28DRAFT_3181 overlap by 425 nucleotides EX28DRAFT_3181 and EX28DRAFT_3182 are separated by 59 nucleotides EX28DRAFT_3182 and EX28DRAFT_3183 are separated by 21 nucleotides
EX28DRAFT_3179: EX28DRAFT_3179 - Transcriptional regulator, at 480,325 to 481,245
_3179
EX28DRAFT_3180: EX28DRAFT_3180 - hypothetical protein, at 481,831 to 482,346
_3180
EX28DRAFT_3181: EX28DRAFT_3181 - RNA, at 481,922 to 482,298
_3181
EX28DRAFT_3182: EX28DRAFT_3182 - 2-hydroxy-3-oxopropionate reductase, at 482,358 to 483,248
_3182
EX28DRAFT_3183: EX28DRAFT_3183 - 2-dehydro-3-deoxyglucarate aldolase, at 483,270 to 484,040
_3183
Position (kb) 481
482
483 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 481.077 kb on + strand, within EX28DRAFT_3179 at 481.104 kb on - strand, within EX28DRAFT_3179 at 481.188 kb on + strand at 481.233 kb on + strand at 481.284 kb on + strand at 481.657 kb on - strand at 481.658 kb on + strand at 481.658 kb on + strand at 481.658 kb on + strand at 481.659 kb on - strand at 481.693 kb on + strand at 481.776 kb on + strand at 482.337 kb on + strand at 482.338 kb on - strand at 482.344 kb on + strand at 482.345 kb on - strand at 482.395 kb on + strand at 482.395 kb on + strand at 482.534 kb on + strand, within EX28DRAFT_3182 at 482.568 kb on - strand, within EX28DRAFT_3182 at 482.607 kb on - strand, within EX28DRAFT_3182 at 482.803 kb on + strand, within EX28DRAFT_3182 at 482.803 kb on + strand, within EX28DRAFT_3182 at 482.803 kb on + strand, within EX28DRAFT_3182 at 482.803 kb on + strand, within EX28DRAFT_3182 at 482.804 kb on - strand, within EX28DRAFT_3182 at 482.804 kb on - strand, within EX28DRAFT_3182 at 482.806 kb on + strand, within EX28DRAFT_3182 at 482.806 kb on + strand, within EX28DRAFT_3182 at 482.806 kb on + strand, within EX28DRAFT_3182 at 482.807 kb on - strand, within EX28DRAFT_3182 at 482.807 kb on - strand, within EX28DRAFT_3182 at 482.957 kb on + strand, within EX28DRAFT_3182 at 482.957 kb on + strand, within EX28DRAFT_3182 at 482.957 kb on + strand, within EX28DRAFT_3182 at 482.966 kb on + strand, within EX28DRAFT_3182 at 483.173 kb on - strand at 483.194 kb on + strand at 483.195 kb on - strand at 483.248 kb on + strand at 483.249 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 481,077 + EX28DRAFT_3179 0.82 +0.0 481,104 - EX28DRAFT_3179 0.85 +0.2 481,188 + +0.4 481,233 + -2.2 481,284 + +1.0 481,657 - -2.9 481,658 + +0.8 481,658 + +0.7 481,658 + -0.4 481,659 - -1.0 481,693 + -0.2 481,776 + +0.3 482,337 + -0.1 482,338 - +1.2 482,344 + +0.2 482,345 - +0.6 482,395 + +0.3 482,395 + +0.3 482,534 + EX28DRAFT_3182 0.20 +4.2 482,568 - EX28DRAFT_3182 0.24 +0.0 482,607 - EX28DRAFT_3182 0.28 +0.3 482,803 + EX28DRAFT_3182 0.50 +0.4 482,803 + EX28DRAFT_3182 0.50 +0.4 482,803 + EX28DRAFT_3182 0.50 -0.8 482,803 + EX28DRAFT_3182 0.50 +0.9 482,804 - EX28DRAFT_3182 0.50 +1.7 482,804 - EX28DRAFT_3182 0.50 -1.1 482,806 + EX28DRAFT_3182 0.50 +0.0 482,806 + EX28DRAFT_3182 0.50 -0.1 482,806 + EX28DRAFT_3182 0.50 -0.5 482,807 - EX28DRAFT_3182 0.50 +0.9 482,807 - EX28DRAFT_3182 0.50 -1.1 482,957 + EX28DRAFT_3182 0.67 -0.5 482,957 + EX28DRAFT_3182 0.67 -1.0 482,957 + EX28DRAFT_3182 0.67 -0.4 482,966 + EX28DRAFT_3182 0.68 -2.3 483,173 - +0.6 483,194 + -0.3 483,195 - -2.3 483,248 + +0.3 483,249 - -0.1
Or see this region's nucleotide sequence