Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3175 and EX28DRAFT_3176 are separated by 15 nucleotides EX28DRAFT_3176 and EX28DRAFT_3177 are separated by 30 nucleotides
EX28DRAFT_3175: EX28DRAFT_3175 - formate acetyltransferase 1, at 474,345 to 476,639
_3175
EX28DRAFT_3176: EX28DRAFT_3176 - acetate kinase, at 476,655 to 477,863
_3176
EX28DRAFT_3177: EX28DRAFT_3177 - serine transporter, at 477,894 to 479,225
_3177
Position (kb) 476
477
478 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 475.768 kb on + strand, within EX28DRAFT_3175 at 475.768 kb on + strand, within EX28DRAFT_3175 at 475.768 kb on + strand, within EX28DRAFT_3175 at 475.769 kb on - strand, within EX28DRAFT_3175 at 475.769 kb on - strand, within EX28DRAFT_3175 at 475.769 kb on - strand, within EX28DRAFT_3175 at 475.849 kb on + strand, within EX28DRAFT_3175 at 475.906 kb on + strand, within EX28DRAFT_3175 at 475.906 kb on + strand, within EX28DRAFT_3175 at 476.024 kb on + strand, within EX28DRAFT_3175 at 476.066 kb on - strand, within EX28DRAFT_3175 at 476.221 kb on + strand, within EX28DRAFT_3175 at 476.251 kb on + strand, within EX28DRAFT_3175 at 476.362 kb on + strand, within EX28DRAFT_3175 at 476.454 kb on + strand at 476.519 kb on - strand at 476.527 kb on + strand at 476.527 kb on + strand at 476.528 kb on - strand at 476.528 kb on - strand at 476.531 kb on + strand at 476.532 kb on - strand at 476.662 kb on + strand at 476.942 kb on + strand, within EX28DRAFT_3176 at 476.942 kb on + strand, within EX28DRAFT_3176 at 476.942 kb on + strand, within EX28DRAFT_3176 at 476.973 kb on + strand, within EX28DRAFT_3176 at 477.168 kb on + strand, within EX28DRAFT_3176 at 477.203 kb on + strand, within EX28DRAFT_3176 at 477.323 kb on - strand, within EX28DRAFT_3176 at 477.400 kb on + strand, within EX28DRAFT_3176 at 477.400 kb on + strand, within EX28DRAFT_3176 at 477.442 kb on - strand, within EX28DRAFT_3176 at 477.505 kb on + strand, within EX28DRAFT_3176 at 477.509 kb on + strand, within EX28DRAFT_3176 at 477.836 kb on + strand at 478.006 kb on + strand at 478.006 kb on + strand at 478.006 kb on + strand at 478.006 kb on + strand at 478.007 kb on - strand at 478.007 kb on - strand at 478.062 kb on - strand, within EX28DRAFT_3177 at 478.062 kb on - strand, within EX28DRAFT_3177 at 478.277 kb on - strand, within EX28DRAFT_3177 at 478.410 kb on - strand, within EX28DRAFT_3177 at 478.410 kb on - strand, within EX28DRAFT_3177 at 478.758 kb on - strand, within EX28DRAFT_3177 at 478.856 kb on + strand, within EX28DRAFT_3177
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 475,768 + EX28DRAFT_3175 0.62 +0.2 475,768 + EX28DRAFT_3175 0.62 -0.4 475,768 + EX28DRAFT_3175 0.62 -0.3 475,769 - EX28DRAFT_3175 0.62 +0.4 475,769 - EX28DRAFT_3175 0.62 +0.3 475,769 - EX28DRAFT_3175 0.62 -0.2 475,849 + EX28DRAFT_3175 0.66 -0.5 475,906 + EX28DRAFT_3175 0.68 +0.1 475,906 + EX28DRAFT_3175 0.68 -2.1 476,024 + EX28DRAFT_3175 0.73 -3.4 476,066 - EX28DRAFT_3175 0.75 -0.0 476,221 + EX28DRAFT_3175 0.82 +0.6 476,251 + EX28DRAFT_3175 0.83 -1.0 476,362 + EX28DRAFT_3175 0.88 -0.3 476,454 + -2.7 476,519 - -0.2 476,527 + +0.0 476,527 + +0.5 476,528 - -0.4 476,528 - +1.2 476,531 + +1.6 476,532 - -2.5 476,662 + +0.4 476,942 + EX28DRAFT_3176 0.24 +0.2 476,942 + EX28DRAFT_3176 0.24 -1.7 476,942 + EX28DRAFT_3176 0.24 -0.6 476,973 + EX28DRAFT_3176 0.26 -0.0 477,168 + EX28DRAFT_3176 0.42 -1.6 477,203 + EX28DRAFT_3176 0.45 -0.1 477,323 - EX28DRAFT_3176 0.55 +0.7 477,400 + EX28DRAFT_3176 0.62 +1.0 477,400 + EX28DRAFT_3176 0.62 +0.2 477,442 - EX28DRAFT_3176 0.65 +0.0 477,505 + EX28DRAFT_3176 0.70 +1.0 477,509 + EX28DRAFT_3176 0.71 +0.9 477,836 + -0.3 478,006 + -0.8 478,006 + +0.5 478,006 + -0.9 478,006 + +0.9 478,007 - -0.0 478,007 - -2.5 478,062 - EX28DRAFT_3177 0.13 +0.0 478,062 - EX28DRAFT_3177 0.13 +0.5 478,277 - EX28DRAFT_3177 0.29 -1.7 478,410 - EX28DRAFT_3177 0.39 -0.4 478,410 - EX28DRAFT_3177 0.39 +0.5 478,758 - EX28DRAFT_3177 0.65 -0.7 478,856 + EX28DRAFT_3177 0.72 -1.7
Or see this region's nucleotide sequence