Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3123 and EX28DRAFT_3124 overlap by 22 nucleotides EX28DRAFT_3124 and EX28DRAFT_3125 overlap by 17 nucleotides EX28DRAFT_3125 and EX28DRAFT_3126 overlap by 10 nucleotides EX28DRAFT_3126 and EX28DRAFT_3127 are separated by 179 nucleotides EX28DRAFT_3127 and EX28DRAFT_3128 are separated by 67 nucleotides
EX28DRAFT_3123: EX28DRAFT_3123 - phage lysis regulatory protein, LysB family, at 427,492 to 427,935
_3123
EX28DRAFT_3124: EX28DRAFT_3124 - Phage-related lysozyme (muraminidase), at 427,914 to 428,423
_3124
EX28DRAFT_3125: EX28DRAFT_3125 - holin, HP1 family, at 428,407 to 428,628
_3125
EX28DRAFT_3126: EX28DRAFT_3126 - P2-like prophage tail protein X, at 428,619 to 428,822
_3126
EX28DRAFT_3127: EX28DRAFT_3127 - DinI-like family, at 429,002 to 429,484
_3127
EX28DRAFT_3128: EX28DRAFT_3128 - Bacteriophage replication gene A protein (GPA), at 429,552 to 431,741
_3128
Position (kb) 428
429 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 428.036 kb on + strand, within EX28DRAFT_3124 at 428.097 kb on - strand, within EX28DRAFT_3124 at 428.142 kb on + strand, within EX28DRAFT_3124 at 428.277 kb on - strand, within EX28DRAFT_3124 at 428.350 kb on - strand, within EX28DRAFT_3124 at 428.628 kb on + strand at 428.657 kb on + strand, within EX28DRAFT_3126 at 428.812 kb on + strand at 428.812 kb on + strand at 428.813 kb on - strand at 428.856 kb on + strand at 428.857 kb on - strand at 429.146 kb on + strand, within EX28DRAFT_3127 at 429.286 kb on + strand, within EX28DRAFT_3127 at 429.466 kb on + strand at 429.492 kb on + strand at 429.533 kb on + strand at 429.534 kb on - strand at 429.677 kb on + strand at 429.678 kb on - strand at 429.678 kb on - strand at 429.738 kb on + strand at 429.756 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 428,036 + EX28DRAFT_3124 0.24 +0.2 428,097 - EX28DRAFT_3124 0.36 -0.0 428,142 + EX28DRAFT_3124 0.45 +1.0 428,277 - EX28DRAFT_3124 0.71 -1.7 428,350 - EX28DRAFT_3124 0.85 +0.1 428,628 + -1.1 428,657 + EX28DRAFT_3126 0.19 -0.4 428,812 + +0.0 428,812 + -0.4 428,813 - -0.9 428,856 + +0.1 428,857 - -0.6 429,146 + EX28DRAFT_3127 0.30 +0.2 429,286 + EX28DRAFT_3127 0.59 -0.6 429,466 + +0.6 429,492 + +0.8 429,533 + +0.5 429,534 - +2.9 429,677 + +0.0 429,678 - +0.5 429,678 - -3.7 429,738 + +0.8 429,756 + -1.1
Or see this region's nucleotide sequence