Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2815 and EX28DRAFT_2816 are separated by 69 nucleotides EX28DRAFT_2816 and EX28DRAFT_2817 are separated by 211 nucleotides EX28DRAFT_2817 and EX28DRAFT_2818 are separated by 3 nucleotides EX28DRAFT_2818 and EX28DRAFT_2819 overlap by 10 nucleotides
EX28DRAFT_2815: EX28DRAFT_2815 - Fe-S-cluster-containing hydrogenase components 2, at 119,458 to 120,066
_2815
EX28DRAFT_2816: EX28DRAFT_2816 - Transcriptional repressor of hyc and hyp operons, at 120,136 to 120,600
_2816
EX28DRAFT_2817: EX28DRAFT_2817 - hydrogenase nickel insertion protein HypA, at 120,812 to 121,162
_2817
EX28DRAFT_2818: EX28DRAFT_2818 - hydrogenase accessory protein HypB, at 121,166 to 122,029
_2818
EX28DRAFT_2819: EX28DRAFT_2819 - hydrogenase assembly chaperone HypC/HupF, at 122,020 to 122,292
_2819
Position (kb)
120
121
122 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 120.085 kb on + strand at 120.086 kb on - strand at 120.212 kb on + strand, within EX28DRAFT_2816 at 120.224 kb on + strand, within EX28DRAFT_2816 at 120.225 kb on - strand, within EX28DRAFT_2816 at 120.249 kb on - strand, within EX28DRAFT_2816 at 120.330 kb on + strand, within EX28DRAFT_2816 at 120.330 kb on + strand, within EX28DRAFT_2816 at 120.497 kb on - strand, within EX28DRAFT_2816 at 120.567 kb on - strand at 120.595 kb on - strand at 120.600 kb on + strand at 120.601 kb on - strand at 120.601 kb on - strand at 121.046 kb on + strand, within EX28DRAFT_2817 at 121.047 kb on - strand, within EX28DRAFT_2817 at 121.047 kb on - strand, within EX28DRAFT_2817 at 121.047 kb on - strand, within EX28DRAFT_2817 at 121.047 kb on - strand, within EX28DRAFT_2817 at 121.047 kb on - strand, within EX28DRAFT_2817 at 121.047 kb on - strand, within EX28DRAFT_2817 at 121.165 kb on + strand at 121.166 kb on - strand at 121.166 kb on - strand at 121.166 kb on - strand at 121.166 kb on - strand at 121.171 kb on + strand at 121.172 kb on - strand at 121.202 kb on + strand at 121.202 kb on + strand at 121.202 kb on + strand at 121.202 kb on + strand at 121.202 kb on + strand at 121.203 kb on - strand at 121.203 kb on - strand at 121.203 kb on - strand at 121.203 kb on - strand at 121.203 kb on - strand at 121.203 kb on - strand at 121.203 kb on - strand at 121.203 kb on - strand at 121.204 kb on + strand at 121.204 kb on + strand at 121.206 kb on + strand at 121.206 kb on + strand at 121.206 kb on + strand at 121.206 kb on + strand at 121.206 kb on + strand at 121.206 kb on + strand at 121.206 kb on + strand at 121.206 kb on + strand at 121.206 kb on + strand at 121.207 kb on - strand at 121.207 kb on - strand at 121.207 kb on - strand at 121.207 kb on - strand at 121.207 kb on - strand at 121.837 kb on + strand, within EX28DRAFT_2818 at 121.837 kb on + strand, within EX28DRAFT_2818 at 121.838 kb on - strand, within EX28DRAFT_2818 at 121.889 kb on + strand, within EX28DRAFT_2818 at 121.905 kb on + strand, within EX28DRAFT_2818 at 121.906 kb on - strand, within EX28DRAFT_2818 at 121.909 kb on + strand, within EX28DRAFT_2818 at 121.909 kb on + strand, within EX28DRAFT_2818 at 121.909 kb on + strand, within EX28DRAFT_2818 at 121.910 kb on - strand, within EX28DRAFT_2818 at 121.910 kb on - strand, within EX28DRAFT_2818 at 121.910 kb on - strand, within EX28DRAFT_2818 at 121.910 kb on - strand, within EX28DRAFT_2818 at 121.922 kb on + strand, within EX28DRAFT_2818 at 121.922 kb on + strand, within EX28DRAFT_2818 at 121.923 kb on - strand, within EX28DRAFT_2818 at 122.028 kb on - strand at 122.028 kb on - strand at 122.047 kb on + strand at 122.048 kb on - strand, within EX28DRAFT_2819 at 122.048 kb on - strand, within EX28DRAFT_2819
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 120,085 + +0.3 120,086 - -2.1 120,212 + EX28DRAFT_2816 0.16 -1.0 120,224 + EX28DRAFT_2816 0.19 +1.5 120,225 - EX28DRAFT_2816 0.19 -0.5 120,249 - EX28DRAFT_2816 0.24 +0.2 120,330 + EX28DRAFT_2816 0.42 -0.3 120,330 + EX28DRAFT_2816 0.42 +0.5 120,497 - EX28DRAFT_2816 0.78 -0.2 120,567 - +0.9 120,595 - +1.3 120,600 + +0.2 120,601 - -1.8 120,601 - -0.1 121,046 + EX28DRAFT_2817 0.67 -0.7 121,047 - EX28DRAFT_2817 0.67 -0.0 121,047 - EX28DRAFT_2817 0.67 +0.1 121,047 - EX28DRAFT_2817 0.67 -0.7 121,047 - EX28DRAFT_2817 0.67 -0.9 121,047 - EX28DRAFT_2817 0.67 +1.2 121,047 - EX28DRAFT_2817 0.67 +0.3 121,165 + -0.5 121,166 - +0.6 121,166 - +0.5 121,166 - -0.6 121,166 - +1.9 121,171 + +0.4 121,172 - +0.0 121,202 + -0.1 121,202 + -0.3 121,202 + -3.2 121,202 + +0.3 121,202 + +1.0 121,203 - -0.8 121,203 - -0.1 121,203 - +0.5 121,203 - +0.3 121,203 - -0.1 121,203 - +0.6 121,203 - -4.0 121,203 - +0.2 121,204 + -3.3 121,204 + +0.0 121,206 + -0.7 121,206 + -0.3 121,206 + +0.7 121,206 + -2.6 121,206 + +0.1 121,206 + +0.7 121,206 + +0.4 121,206 + -1.2 121,206 + +0.4 121,207 - +0.2 121,207 - +0.1 121,207 - +0.2 121,207 - -0.4 121,207 - +0.1 121,837 + EX28DRAFT_2818 0.78 +0.7 121,837 + EX28DRAFT_2818 0.78 -0.5 121,838 - EX28DRAFT_2818 0.78 -0.7 121,889 + EX28DRAFT_2818 0.84 -1.3 121,905 + EX28DRAFT_2818 0.86 +0.8 121,906 - EX28DRAFT_2818 0.86 +0.5 121,909 + EX28DRAFT_2818 0.86 +0.4 121,909 + EX28DRAFT_2818 0.86 -0.1 121,909 + EX28DRAFT_2818 0.86 -0.5 121,910 - EX28DRAFT_2818 0.86 -0.9 121,910 - EX28DRAFT_2818 0.86 -0.5 121,910 - EX28DRAFT_2818 0.86 -0.4 121,910 - EX28DRAFT_2818 0.86 +1.9 121,922 + EX28DRAFT_2818 0.88 -0.5 121,922 + EX28DRAFT_2818 0.88 +0.4 121,923 - EX28DRAFT_2818 0.88 +0.5 122,028 - +0.3 122,028 - +0.3 122,047 + -0.6 122,048 - EX28DRAFT_2819 0.10 -1.2 122,048 - EX28DRAFT_2819 0.10 +0.0
Or see this region's nucleotide sequence