Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2752 and EX28DRAFT_2753 are separated by 9 nucleotides EX28DRAFT_2753 and EX28DRAFT_2754 are separated by 79 nucleotides EX28DRAFT_2754 and EX28DRAFT_2755 are separated by 37 nucleotides EX28DRAFT_2755 and EX28DRAFT_2756 are separated by 495 nucleotides EX28DRAFT_2756 and EX28DRAFT_2757 are separated by 32 nucleotides
EX28DRAFT_2752: EX28DRAFT_2752 - Predicted transcriptional regulators, at 62,579 to 62,878
_2752
EX28DRAFT_2753: EX28DRAFT_2753 - Rhodanese-related sulfurtransferase, at 62,888 to 63,409
_2753
EX28DRAFT_2754: EX28DRAFT_2754 - hypothetical protein, at 63,489 to 63,668
_2754
EX28DRAFT_2755: EX28DRAFT_2755 - DNA-binding protein H-NS, at 63,706 to 64,110
_2755
EX28DRAFT_2756: EX28DRAFT_2756 - Protein of unknown function (DUF1144), at 64,606 to 65,055
_2756
EX28DRAFT_2757: EX28DRAFT_2757 - Protein of unknown function (DUF2002), at 65,088 to 65,432
_2757
Position (kb) 63
64
65 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 62.728 kb on - strand, within EX28DRAFT_2752 at 62.814 kb on - strand, within EX28DRAFT_2752 at 62.835 kb on + strand, within EX28DRAFT_2752 at 62.868 kb on - strand at 63.037 kb on + strand, within EX28DRAFT_2753 at 63.278 kb on + strand, within EX28DRAFT_2753 at 63.414 kb on + strand at 63.426 kb on + strand at 63.485 kb on + strand at 63.486 kb on - strand at 63.486 kb on - strand at 63.486 kb on - strand at 63.488 kb on + strand at 63.507 kb on + strand, within EX28DRAFT_2754 at 63.508 kb on - strand, within EX28DRAFT_2754 at 63.508 kb on - strand, within EX28DRAFT_2754 at 63.553 kb on + strand, within EX28DRAFT_2754 at 63.553 kb on + strand, within EX28DRAFT_2754 at 63.554 kb on - strand, within EX28DRAFT_2754 at 63.606 kb on + strand, within EX28DRAFT_2754 at 63.606 kb on + strand, within EX28DRAFT_2754 at 63.606 kb on + strand, within EX28DRAFT_2754 at 63.607 kb on - strand, within EX28DRAFT_2754 at 63.608 kb on + strand, within EX28DRAFT_2754 at 63.628 kb on + strand, within EX28DRAFT_2754 at 63.822 kb on - strand, within EX28DRAFT_2755 at 63.971 kb on - strand, within EX28DRAFT_2755 at 64.048 kb on + strand, within EX28DRAFT_2755 at 64.084 kb on - strand at 64.087 kb on - strand at 64.114 kb on - strand at 64.114 kb on - strand at 64.144 kb on - strand at 64.147 kb on + strand at 64.148 kb on - strand at 64.182 kb on + strand at 64.214 kb on + strand at 64.215 kb on - strand at 64.218 kb on + strand at 64.221 kb on - strand at 64.294 kb on - strand at 64.500 kb on - strand at 64.519 kb on - strand at 64.525 kb on + strand at 64.697 kb on + strand, within EX28DRAFT_2756 at 64.697 kb on + strand, within EX28DRAFT_2756 at 64.762 kb on - strand, within EX28DRAFT_2756 at 64.765 kb on - strand, within EX28DRAFT_2756 at 64.781 kb on - strand, within EX28DRAFT_2756 at 64.867 kb on + strand, within EX28DRAFT_2756 at 64.868 kb on - strand, within EX28DRAFT_2756 at 65.023 kb on - strand at 65.035 kb on + strand at 65.090 kb on - strand at 65.094 kb on + strand at 65.094 kb on + strand at 65.095 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 62,728 - EX28DRAFT_2752 0.50 -2.4 62,814 - EX28DRAFT_2752 0.78 +0.9 62,835 + EX28DRAFT_2752 0.85 -0.5 62,868 - +0.1 63,037 + EX28DRAFT_2753 0.29 +0.9 63,278 + EX28DRAFT_2753 0.75 -4.2 63,414 + +0.5 63,426 + -1.0 63,485 + -0.8 63,486 - -0.1 63,486 - +0.9 63,486 - +0.1 63,488 + -0.1 63,507 + EX28DRAFT_2754 0.10 +0.2 63,508 - EX28DRAFT_2754 0.11 -2.1 63,508 - EX28DRAFT_2754 0.11 -1.3 63,553 + EX28DRAFT_2754 0.36 +0.1 63,553 + EX28DRAFT_2754 0.36 +0.2 63,554 - EX28DRAFT_2754 0.36 +0.9 63,606 + EX28DRAFT_2754 0.65 -1.1 63,606 + EX28DRAFT_2754 0.65 -0.5 63,606 + EX28DRAFT_2754 0.65 +0.8 63,607 - EX28DRAFT_2754 0.66 +1.2 63,608 + EX28DRAFT_2754 0.66 +0.2 63,628 + EX28DRAFT_2754 0.77 +1.1 63,822 - EX28DRAFT_2755 0.29 +0.6 63,971 - EX28DRAFT_2755 0.65 -0.4 64,048 + EX28DRAFT_2755 0.84 +0.7 64,084 - -2.4 64,087 - +0.1 64,114 - -0.1 64,114 - -3.2 64,144 - -0.0 64,147 + +2.0 64,148 - +0.1 64,182 + +0.3 64,214 + -1.8 64,215 - +0.7 64,218 + +0.3 64,221 - -0.3 64,294 - +0.3 64,500 - +0.2 64,519 - -0.4 64,525 + +0.2 64,697 + EX28DRAFT_2756 0.20 -0.5 64,697 + EX28DRAFT_2756 0.20 +0.2 64,762 - EX28DRAFT_2756 0.35 -0.6 64,765 - EX28DRAFT_2756 0.35 +1.3 64,781 - EX28DRAFT_2756 0.39 +0.5 64,867 + EX28DRAFT_2756 0.58 -0.2 64,868 - EX28DRAFT_2756 0.58 +1.0 65,023 - -0.6 65,035 + -0.2 65,090 - +0.1 65,094 + -0.2 65,094 + -0.6 65,095 - +1.7
Or see this region's nucleotide sequence