Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2365 and EX28DRAFT_2366 are separated by 183 nucleotides EX28DRAFT_2366 and EX28DRAFT_2367 overlap by 29 nucleotides EX28DRAFT_2367 and EX28DRAFT_2368 overlap by 4 nucleotides EX28DRAFT_2368 and EX28DRAFT_2369 are separated by 96 nucleotides
EX28DRAFT_2365: EX28DRAFT_2365 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 2,386,377 to 2,387,120
_2365
EX28DRAFT_2366: EX28DRAFT_2366 - Cytotoxic translational repressor of toxin-antitoxin stability system, at 2,387,304 to 2,387,609
_2366
EX28DRAFT_2367: EX28DRAFT_2367 - Helix-turn-helix, at 2,387,581 to 2,387,985
_2367
EX28DRAFT_2368: EX28DRAFT_2368 - Uncharacterized small protein, at 2,387,982 to 2,388,179
_2368
EX28DRAFT_2369: EX28DRAFT_2369 - Carbon starvation protein, predicted membrane protein, at 2,388,276 to 2,390,381
_2369
Position (kb)
2387
2388 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2386.787 kb on + strand, within EX28DRAFT_2365 at 2386.824 kb on - strand, within EX28DRAFT_2365 at 2386.953 kb on - strand, within EX28DRAFT_2365 at 2387.219 kb on + strand at 2387.219 kb on + strand at 2387.252 kb on - strand at 2387.261 kb on - strand at 2387.263 kb on + strand at 2387.264 kb on - strand at 2387.288 kb on + strand at 2387.288 kb on + strand at 2387.300 kb on - strand at 2387.487 kb on + strand, within EX28DRAFT_2366 at 2387.489 kb on + strand, within EX28DRAFT_2366 at 2387.489 kb on + strand, within EX28DRAFT_2366 at 2387.490 kb on - strand, within EX28DRAFT_2366 at 2387.580 kb on + strand at 2387.581 kb on - strand at 2387.588 kb on + strand at 2387.588 kb on + strand at 2387.589 kb on - strand at 2387.595 kb on - strand at 2387.623 kb on + strand, within EX28DRAFT_2367 at 2387.623 kb on + strand, within EX28DRAFT_2367 at 2387.624 kb on - strand, within EX28DRAFT_2367 at 2387.624 kb on - strand, within EX28DRAFT_2367 at 2387.624 kb on - strand, within EX28DRAFT_2367 at 2387.655 kb on + strand, within EX28DRAFT_2367 at 2387.655 kb on + strand, within EX28DRAFT_2367 at 2387.663 kb on + strand, within EX28DRAFT_2367 at 2387.663 kb on + strand, within EX28DRAFT_2367 at 2387.727 kb on + strand, within EX28DRAFT_2367 at 2387.759 kb on + strand, within EX28DRAFT_2367 at 2387.759 kb on + strand, within EX28DRAFT_2367 at 2387.760 kb on - strand, within EX28DRAFT_2367 at 2387.760 kb on - strand, within EX28DRAFT_2367 at 2387.760 kb on - strand, within EX28DRAFT_2367 at 2387.837 kb on + strand, within EX28DRAFT_2367 at 2387.936 kb on - strand, within EX28DRAFT_2367 at 2388.049 kb on + strand, within EX28DRAFT_2368 at 2388.049 kb on + strand, within EX28DRAFT_2368 at 2388.086 kb on + strand, within EX28DRAFT_2368 at 2388.086 kb on + strand, within EX28DRAFT_2368 at 2388.087 kb on - strand, within EX28DRAFT_2368 at 2388.097 kb on + strand, within EX28DRAFT_2368 at 2388.097 kb on + strand, within EX28DRAFT_2368 at 2388.106 kb on + strand, within EX28DRAFT_2368 at 2388.149 kb on - strand, within EX28DRAFT_2368 at 2388.151 kb on + strand, within EX28DRAFT_2368 at 2388.152 kb on + strand, within EX28DRAFT_2368 at 2388.152 kb on + strand, within EX28DRAFT_2368 at 2388.153 kb on - strand, within EX28DRAFT_2368 at 2388.153 kb on - strand, within EX28DRAFT_2368 at 2388.153 kb on - strand, within EX28DRAFT_2368 at 2388.179 kb on + strand at 2388.397 kb on + strand at 2388.397 kb on + strand at 2388.397 kb on + strand at 2388.398 kb on - strand at 2388.425 kb on + strand at 2388.425 kb on + strand at 2388.426 kb on - strand at 2388.426 kb on - strand at 2388.434 kb on + strand at 2388.435 kb on - strand at 2388.435 kb on - strand at 2388.750 kb on - strand, within EX28DRAFT_2369 at 2388.894 kb on - strand, within EX28DRAFT_2369 at 2388.894 kb on - strand, within EX28DRAFT_2369 at 2388.894 kb on - strand, within EX28DRAFT_2369
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 2,386,787 + EX28DRAFT_2365 0.55 +0.1 2,386,824 - EX28DRAFT_2365 0.60 -0.5 2,386,953 - EX28DRAFT_2365 0.77 -0.2 2,387,219 + -0.8 2,387,219 + -1.9 2,387,252 - +0.6 2,387,261 - +0.0 2,387,263 + +1.0 2,387,264 - -1.2 2,387,288 + -1.2 2,387,288 + -0.0 2,387,300 - +0.1 2,387,487 + EX28DRAFT_2366 0.60 -1.5 2,387,489 + EX28DRAFT_2366 0.60 -0.1 2,387,489 + EX28DRAFT_2366 0.60 +0.3 2,387,490 - EX28DRAFT_2366 0.61 -0.9 2,387,580 + +1.4 2,387,581 - -1.3 2,387,588 + -0.7 2,387,588 + -1.9 2,387,589 - +0.6 2,387,595 - -0.3 2,387,623 + EX28DRAFT_2367 0.10 -0.0 2,387,623 + EX28DRAFT_2367 0.10 +0.1 2,387,624 - EX28DRAFT_2367 0.11 -0.7 2,387,624 - EX28DRAFT_2367 0.11 +0.4 2,387,624 - EX28DRAFT_2367 0.11 +1.2 2,387,655 + EX28DRAFT_2367 0.18 +0.0 2,387,655 + EX28DRAFT_2367 0.18 +0.5 2,387,663 + EX28DRAFT_2367 0.20 +0.8 2,387,663 + EX28DRAFT_2367 0.20 -0.8 2,387,727 + EX28DRAFT_2367 0.36 +1.6 2,387,759 + EX28DRAFT_2367 0.44 -0.9 2,387,759 + EX28DRAFT_2367 0.44 +0.6 2,387,760 - EX28DRAFT_2367 0.44 -0.1 2,387,760 - EX28DRAFT_2367 0.44 -0.3 2,387,760 - EX28DRAFT_2367 0.44 +0.2 2,387,837 + EX28DRAFT_2367 0.63 +0.9 2,387,936 - EX28DRAFT_2367 0.88 +0.5 2,388,049 + EX28DRAFT_2368 0.34 -2.5 2,388,049 + EX28DRAFT_2368 0.34 +0.4 2,388,086 + EX28DRAFT_2368 0.53 +0.6 2,388,086 + EX28DRAFT_2368 0.53 -2.5 2,388,087 - EX28DRAFT_2368 0.53 -0.1 2,388,097 + EX28DRAFT_2368 0.58 -3.8 2,388,097 + EX28DRAFT_2368 0.58 -1.2 2,388,106 + EX28DRAFT_2368 0.63 +0.3 2,388,149 - EX28DRAFT_2368 0.84 +0.6 2,388,151 + EX28DRAFT_2368 0.85 -0.5 2,388,152 + EX28DRAFT_2368 0.86 -2.2 2,388,152 + EX28DRAFT_2368 0.86 +0.9 2,388,153 - EX28DRAFT_2368 0.86 -0.2 2,388,153 - EX28DRAFT_2368 0.86 -0.5 2,388,153 - EX28DRAFT_2368 0.86 +0.1 2,388,179 + +0.0 2,388,397 + +0.3 2,388,397 + +0.8 2,388,397 + -1.4 2,388,398 - -4.2 2,388,425 + +0.1 2,388,425 + +0.0 2,388,426 - +1.1 2,388,426 - +0.1 2,388,434 + -0.3 2,388,435 - +0.5 2,388,435 - +0.4 2,388,750 - EX28DRAFT_2369 0.23 +0.2 2,388,894 - EX28DRAFT_2369 0.29 -0.6 2,388,894 - EX28DRAFT_2369 0.29 +0.6 2,388,894 - EX28DRAFT_2369 0.29 +0.1
Or see this region's nucleotide sequence