Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1714 and EX28DRAFT_1715 are separated by 294 nucleotides EX28DRAFT_1715 and EX28DRAFT_1716 are separated by 74 nucleotides EX28DRAFT_1716 and EX28DRAFT_1717 overlap by 1 nucleotides EX28DRAFT_1717 and EX28DRAFT_1718 are separated by 34 nucleotides
EX28DRAFT_1714: EX28DRAFT_1714 - FOG: EAL domain, at 1,730,968 to 1,731,684
_1714
EX28DRAFT_1715: EX28DRAFT_1715 - Cell wall-associated hydrolases (invasion-associated proteins), at 1,731,979 to 1,732,443
_1715
EX28DRAFT_1716: EX28DRAFT_1716 - ABC-type cobalamin transport system, ATPase component, at 1,732,518 to 1,733,273
_1716
EX28DRAFT_1717: EX28DRAFT_1717 - Glutathione peroxidase, at 1,733,273 to 1,733,824
_1717
EX28DRAFT_1718: EX28DRAFT_1718 - ABC-type cobalamin transport system, permease component, at 1,733,859 to 1,734,839
_1718
Position (kb)
1732
1733
1734 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1731.840 kb on + strand at 1731.938 kb on - strand at 1731.940 kb on + strand at 1731.944 kb on + strand at 1731.957 kb on + strand at 1731.958 kb on - strand at 1731.980 kb on + strand at 1731.981 kb on - strand at 1731.983 kb on - strand at 1731.983 kb on - strand at 1731.983 kb on - strand at 1731.983 kb on - strand at 1731.985 kb on - strand at 1731.985 kb on - strand at 1731.985 kb on - strand at 1731.985 kb on - strand at 1731.985 kb on - strand at 1732.181 kb on - strand, within EX28DRAFT_1715 at 1732.217 kb on - strand, within EX28DRAFT_1715 at 1732.217 kb on - strand, within EX28DRAFT_1715 at 1732.217 kb on - strand, within EX28DRAFT_1715 at 1732.227 kb on + strand, within EX28DRAFT_1715 at 1732.520 kb on - strand at 1732.533 kb on - strand at 1732.547 kb on + strand at 1732.547 kb on + strand at 1732.547 kb on + strand at 1732.547 kb on + strand at 1732.548 kb on - strand at 1732.558 kb on - strand at 1732.793 kb on + strand, within EX28DRAFT_1716 at 1732.793 kb on + strand, within EX28DRAFT_1716 at 1732.830 kb on - strand, within EX28DRAFT_1716 at 1732.830 kb on - strand, within EX28DRAFT_1716 at 1732.920 kb on - strand, within EX28DRAFT_1716 at 1733.045 kb on - strand, within EX28DRAFT_1716 at 1733.274 kb on + strand at 1733.566 kb on - strand, within EX28DRAFT_1717 at 1733.588 kb on - strand, within EX28DRAFT_1717 at 1733.738 kb on - strand, within EX28DRAFT_1717 at 1733.738 kb on - strand, within EX28DRAFT_1717 at 1733.875 kb on - strand at 1733.878 kb on - strand at 1733.878 kb on - strand at 1733.878 kb on - strand at 1733.888 kb on + strand at 1733.967 kb on - strand, within EX28DRAFT_1718 at 1734.126 kb on - strand, within EX28DRAFT_1718 at 1734.126 kb on - strand, within EX28DRAFT_1718 at 1734.129 kb on + strand, within EX28DRAFT_1718 at 1734.130 kb on - strand, within EX28DRAFT_1718 at 1734.130 kb on - strand, within EX28DRAFT_1718 at 1734.130 kb on - strand, within EX28DRAFT_1718 at 1734.130 kb on - strand, within EX28DRAFT_1718 at 1734.130 kb on - strand, within EX28DRAFT_1718 at 1734.130 kb on - strand, within EX28DRAFT_1718 at 1734.132 kb on + strand, within EX28DRAFT_1718 at 1734.132 kb on + strand, within EX28DRAFT_1718 at 1734.133 kb on - strand, within EX28DRAFT_1718 at 1734.203 kb on + strand, within EX28DRAFT_1718 at 1734.204 kb on - strand, within EX28DRAFT_1718 at 1734.204 kb on - strand, within EX28DRAFT_1718
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,731,840 + +0.6 1,731,938 - +0.2 1,731,940 + +0.4 1,731,944 + -1.3 1,731,957 + +0.2 1,731,958 - +0.4 1,731,980 + +2.1 1,731,981 - +1.5 1,731,983 - +0.3 1,731,983 - +0.8 1,731,983 - -0.1 1,731,983 - +0.4 1,731,985 - -0.1 1,731,985 - -0.0 1,731,985 - -0.2 1,731,985 - -1.3 1,731,985 - -0.8 1,732,181 - EX28DRAFT_1715 0.43 -1.5 1,732,217 - EX28DRAFT_1715 0.51 -1.9 1,732,217 - EX28DRAFT_1715 0.51 -0.5 1,732,217 - EX28DRAFT_1715 0.51 -0.0 1,732,227 + EX28DRAFT_1715 0.53 +0.2 1,732,520 - +0.2 1,732,533 - +0.1 1,732,547 + -0.7 1,732,547 + +0.1 1,732,547 + -1.1 1,732,547 + -0.4 1,732,548 - -0.0 1,732,558 - -0.2 1,732,793 + EX28DRAFT_1716 0.36 -1.3 1,732,793 + EX28DRAFT_1716 0.36 -1.1 1,732,830 - EX28DRAFT_1716 0.41 -0.6 1,732,830 - EX28DRAFT_1716 0.41 -0.6 1,732,920 - EX28DRAFT_1716 0.53 -2.3 1,733,045 - EX28DRAFT_1716 0.70 +0.4 1,733,274 + -0.9 1,733,566 - EX28DRAFT_1717 0.53 -0.7 1,733,588 - EX28DRAFT_1717 0.57 +0.1 1,733,738 - EX28DRAFT_1717 0.84 +0.4 1,733,738 - EX28DRAFT_1717 0.84 +0.1 1,733,875 - -3.1 1,733,878 - -0.1 1,733,878 - -0.3 1,733,878 - +1.7 1,733,888 + -0.5 1,733,967 - EX28DRAFT_1718 0.11 +0.5 1,734,126 - EX28DRAFT_1718 0.27 -4.1 1,734,126 - EX28DRAFT_1718 0.27 +0.4 1,734,129 + EX28DRAFT_1718 0.28 -0.3 1,734,130 - EX28DRAFT_1718 0.28 -0.6 1,734,130 - EX28DRAFT_1718 0.28 -1.9 1,734,130 - EX28DRAFT_1718 0.28 +2.4 1,734,130 - EX28DRAFT_1718 0.28 +1.7 1,734,130 - EX28DRAFT_1718 0.28 -0.4 1,734,130 - EX28DRAFT_1718 0.28 +1.6 1,734,132 + EX28DRAFT_1718 0.28 +0.1 1,734,132 + EX28DRAFT_1718 0.28 -0.8 1,734,133 - EX28DRAFT_1718 0.28 +1.9 1,734,203 + EX28DRAFT_1718 0.35 +1.3 1,734,204 - EX28DRAFT_1718 0.35 -2.3 1,734,204 - EX28DRAFT_1718 0.35 +0.9
Or see this region's nucleotide sequence