Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1660 and EX28DRAFT_1661 are separated by 36 nucleotides EX28DRAFT_1661 and EX28DRAFT_1662 are separated by 3 nucleotides EX28DRAFT_1662 and EX28DRAFT_1663 are separated by 4 nucleotides EX28DRAFT_1663 and EX28DRAFT_1664 are separated by 118 nucleotides EX28DRAFT_1664 and EX28DRAFT_1665 are separated by 28 nucleotides EX28DRAFT_1665 and EX28DRAFT_1666 are separated by 72 nucleotides
EX28DRAFT_1660: EX28DRAFT_1660 - putative efflux protein, MATE family, at 1,676,751 to 1,678,112
_1660
EX28DRAFT_1661: EX28DRAFT_1661 - hypothetical protein, at 1,678,149 to 1,678,262
_1661
EX28DRAFT_1662: EX28DRAFT_1662 - tRNA, at 1,678,266 to 1,678,342
_1662
EX28DRAFT_1663: EX28DRAFT_1663 - tRNA, at 1,678,347 to 1,678,423
_1663
EX28DRAFT_1664: EX28DRAFT_1664 - Uncharacterized protein conserved in bacteria, at 1,678,542 to 1,680,137
_1664
EX28DRAFT_1665: EX28DRAFT_1665 - Carbonic anhydrase, at 1,680,166 to 1,680,801
_1665
EX28DRAFT_1666: EX28DRAFT_1666 - hypothetical protein, at 1,680,874 to 1,681,656
_1666
Position (kb) 1678
1679
1680
1681 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 1677.868 kb on + strand, within EX28DRAFT_1660 at 1677.881 kb on + strand, within EX28DRAFT_1660 at 1677.906 kb on - strand, within EX28DRAFT_1660 at 1677.949 kb on - strand, within EX28DRAFT_1660 at 1678.041 kb on - strand at 1678.482 kb on - strand at 1678.584 kb on + strand at 1678.585 kb on - strand at 1678.587 kb on - strand at 1678.671 kb on + strand at 1678.671 kb on + strand at 1678.672 kb on - strand at 1678.685 kb on + strand at 1678.746 kb on + strand, within EX28DRAFT_1664 at 1678.746 kb on + strand, within EX28DRAFT_1664 at 1678.883 kb on - strand, within EX28DRAFT_1664 at 1679.201 kb on + strand, within EX28DRAFT_1664 at 1679.202 kb on - strand, within EX28DRAFT_1664 at 1679.577 kb on + strand, within EX28DRAFT_1664 at 1679.578 kb on - strand, within EX28DRAFT_1664 at 1679.579 kb on + strand, within EX28DRAFT_1664 at 1679.686 kb on - strand, within EX28DRAFT_1664 at 1679.686 kb on - strand, within EX28DRAFT_1664 at 1679.804 kb on - strand, within EX28DRAFT_1664 at 1679.804 kb on - strand, within EX28DRAFT_1664 at 1679.952 kb on + strand, within EX28DRAFT_1664 at 1679.952 kb on + strand, within EX28DRAFT_1664 at 1679.953 kb on - strand, within EX28DRAFT_1664 at 1679.960 kb on - strand, within EX28DRAFT_1664 at 1680.168 kb on - strand at 1680.429 kb on - strand, within EX28DRAFT_1665 at 1680.817 kb on - strand at 1680.817 kb on - strand at 1680.862 kb on - strand at 1680.875 kb on + strand at 1680.876 kb on - strand at 1680.876 kb on - strand at 1680.876 kb on - strand at 1680.891 kb on + strand at 1680.891 kb on + strand at 1680.892 kb on - strand at 1680.892 kb on - strand at 1680.893 kb on + strand at 1680.894 kb on - strand at 1680.894 kb on - strand at 1680.894 kb on - strand at 1681.043 kb on + strand, within EX28DRAFT_1666 at 1681.053 kb on + strand, within EX28DRAFT_1666 at 1681.054 kb on - strand, within EX28DRAFT_1666 at 1681.109 kb on - strand, within EX28DRAFT_1666
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,677,868 + EX28DRAFT_1660 0.82 -2.7 1,677,881 + EX28DRAFT_1660 0.83 -0.9 1,677,906 - EX28DRAFT_1660 0.85 -0.8 1,677,949 - EX28DRAFT_1660 0.88 +0.8 1,678,041 - +0.1 1,678,482 - -0.9 1,678,584 + +2.7 1,678,585 - +3.0 1,678,587 - -0.3 1,678,671 + +0.1 1,678,671 + +0.9 1,678,672 - -0.3 1,678,685 + +0.1 1,678,746 + EX28DRAFT_1664 0.13 +0.7 1,678,746 + EX28DRAFT_1664 0.13 -2.8 1,678,883 - EX28DRAFT_1664 0.21 +0.6 1,679,201 + EX28DRAFT_1664 0.41 -0.9 1,679,202 - EX28DRAFT_1664 0.41 -0.6 1,679,577 + EX28DRAFT_1664 0.65 +0.6 1,679,578 - EX28DRAFT_1664 0.65 +2.2 1,679,579 + EX28DRAFT_1664 0.65 +0.4 1,679,686 - EX28DRAFT_1664 0.72 +2.5 1,679,686 - EX28DRAFT_1664 0.72 -0.9 1,679,804 - EX28DRAFT_1664 0.79 +1.7 1,679,804 - EX28DRAFT_1664 0.79 +0.6 1,679,952 + EX28DRAFT_1664 0.88 +1.2 1,679,952 + EX28DRAFT_1664 0.88 +0.2 1,679,953 - EX28DRAFT_1664 0.88 +1.1 1,679,960 - EX28DRAFT_1664 0.89 +1.1 1,680,168 - -0.0 1,680,429 - EX28DRAFT_1665 0.41 -1.9 1,680,817 - -4.3 1,680,817 - +0.3 1,680,862 - -1.6 1,680,875 + -0.6 1,680,876 - -2.9 1,680,876 - -1.2 1,680,876 - -0.2 1,680,891 + +0.1 1,680,891 + +0.1 1,680,892 - +0.2 1,680,892 - -1.9 1,680,893 + +0.1 1,680,894 - +0.2 1,680,894 - +0.0 1,680,894 - -0.5 1,681,043 + EX28DRAFT_1666 0.22 +0.2 1,681,053 + EX28DRAFT_1666 0.23 -0.6 1,681,054 - EX28DRAFT_1666 0.23 +1.2 1,681,109 - EX28DRAFT_1666 0.30 +0.9
Or see this region's nucleotide sequence